Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5610 and Pf6N2E2_5611 are separated by 114 nucleotides Pf6N2E2_5611 and Pf6N2E2_5612 are separated by 4 nucleotides Pf6N2E2_5612 and Pf6N2E2_5613 are separated by 59 nucleotides
Pf6N2E2_5610 - two-component system sensor protein, at 6,424,936 to 6,426,213
_5610
Pf6N2E2_5611 - SAM-dependent methyltransferases, at 6,426,328 to 6,426,996
_5611
Pf6N2E2_5612 - InaA protein, at 6,427,001 to 6,427,711
_5612
Pf6N2E2_5613 - FIG00953674: hypothetical protein, at 6,427,771 to 6,427,941
_5613
Position (kb)
6426
6427 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 6425.379 kb on - strand, within Pf6N2E2_5610 at 6425.491 kb on + strand, within Pf6N2E2_5610 at 6425.581 kb on - strand, within Pf6N2E2_5610 at 6425.896 kb on + strand, within Pf6N2E2_5610 at 6425.897 kb on - strand, within Pf6N2E2_5610 at 6425.897 kb on - strand, within Pf6N2E2_5610 at 6425.898 kb on + strand, within Pf6N2E2_5610 at 6425.933 kb on + strand, within Pf6N2E2_5610 at 6425.953 kb on + strand, within Pf6N2E2_5610 at 6425.953 kb on + strand, within Pf6N2E2_5610 at 6425.954 kb on - strand, within Pf6N2E2_5610 at 6426.160 kb on - strand at 6426.160 kb on - strand at 6426.160 kb on - strand at 6426.211 kb on + strand at 6426.236 kb on + strand at 6426.263 kb on - strand at 6426.293 kb on + strand at 6426.293 kb on + strand at 6426.294 kb on - strand at 6426.294 kb on - strand at 6426.327 kb on - strand at 6426.396 kb on + strand, within Pf6N2E2_5611 at 6426.633 kb on - strand, within Pf6N2E2_5611 at 6426.863 kb on - strand, within Pf6N2E2_5611 at 6426.891 kb on + strand, within Pf6N2E2_5611 at 6426.891 kb on + strand, within Pf6N2E2_5611 at 6426.891 kb on + strand, within Pf6N2E2_5611 at 6426.891 kb on + strand, within Pf6N2E2_5611 at 6426.891 kb on + strand, within Pf6N2E2_5611 at 6426.891 kb on + strand, within Pf6N2E2_5611 at 6426.892 kb on - strand, within Pf6N2E2_5611 at 6426.892 kb on - strand, within Pf6N2E2_5611 at 6426.892 kb on - strand, within Pf6N2E2_5611 at 6426.892 kb on - strand, within Pf6N2E2_5611 at 6426.892 kb on - strand, within Pf6N2E2_5611 at 6426.895 kb on + strand, within Pf6N2E2_5611 at 6426.896 kb on + strand, within Pf6N2E2_5611 at 6426.896 kb on + strand, within Pf6N2E2_5611 at 6426.896 kb on + strand, within Pf6N2E2_5611 at 6426.896 kb on + strand, within Pf6N2E2_5611 at 6426.897 kb on - strand, within Pf6N2E2_5611 at 6426.897 kb on - strand, within Pf6N2E2_5611 at 6426.897 kb on - strand, within Pf6N2E2_5611 at 6426.959 kb on - strand at 6426.959 kb on - strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.976 kb on + strand at 6426.977 kb on - strand at 6426.977 kb on - strand at 6426.977 kb on - strand at 6426.980 kb on + strand at 6426.980 kb on + strand at 6426.980 kb on + strand at 6426.995 kb on - strand at 6427.008 kb on + strand at 6427.008 kb on + strand at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.090 kb on + strand, within Pf6N2E2_5612 at 6427.091 kb on - strand, within Pf6N2E2_5612 at 6427.096 kb on - strand, within Pf6N2E2_5612 at 6427.154 kb on + strand, within Pf6N2E2_5612 at 6427.154 kb on + strand, within Pf6N2E2_5612 at 6427.155 kb on - strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.177 kb on + strand, within Pf6N2E2_5612 at 6427.178 kb on - strand, within Pf6N2E2_5612 at 6427.178 kb on - strand, within Pf6N2E2_5612 at 6427.181 kb on + strand, within Pf6N2E2_5612 at 6427.181 kb on + strand, within Pf6N2E2_5612 at 6427.373 kb on + strand, within Pf6N2E2_5612 at 6427.374 kb on - strand, within Pf6N2E2_5612 at 6427.483 kb on + strand, within Pf6N2E2_5612 at 6427.483 kb on + strand, within Pf6N2E2_5612 at 6427.483 kb on + strand, within Pf6N2E2_5612 at 6427.483 kb on + strand, within Pf6N2E2_5612 at 6427.483 kb on + strand, within Pf6N2E2_5612 at 6427.483 kb on + strand, within Pf6N2E2_5612 at 6427.487 kb on + strand, within Pf6N2E2_5612 at 6427.487 kb on + strand, within Pf6N2E2_5612 at 6427.487 kb on + strand, within Pf6N2E2_5612 at 6427.487 kb on + strand, within Pf6N2E2_5612 at 6427.488 kb on - strand, within Pf6N2E2_5612 at 6427.488 kb on - strand, within Pf6N2E2_5612 at 6427.488 kb on - strand, within Pf6N2E2_5612 at 6427.488 kb on - strand, within Pf6N2E2_5612 at 6427.746 kb on - strand at 6427.834 kb on + strand, within Pf6N2E2_5613 at 6427.834 kb on + strand, within Pf6N2E2_5613
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 6,425,379 - Pf6N2E2_5610 0.35 -3.6 6,425,491 + Pf6N2E2_5610 0.43 -1.9 6,425,581 - Pf6N2E2_5610 0.50 -4.0 6,425,896 + Pf6N2E2_5610 0.75 -2.6 6,425,897 - Pf6N2E2_5610 0.75 +0.0 6,425,897 - Pf6N2E2_5610 0.75 -1.2 6,425,898 + Pf6N2E2_5610 0.75 -1.8 6,425,933 + Pf6N2E2_5610 0.78 -2.6 6,425,953 + Pf6N2E2_5610 0.80 -2.6 6,425,953 + Pf6N2E2_5610 0.80 -3.2 6,425,954 - Pf6N2E2_5610 0.80 -3.2 6,426,160 - -1.0 6,426,160 - -1.6 6,426,160 - +0.6 6,426,211 + -0.6 6,426,236 + -1.0 6,426,263 - -0.6 6,426,293 + -1.7 6,426,293 + +0.0 6,426,294 - +0.0 6,426,294 - -0.3 6,426,327 - -4.2 6,426,396 + Pf6N2E2_5611 0.10 +0.0 6,426,633 - Pf6N2E2_5611 0.46 -0.4 6,426,863 - Pf6N2E2_5611 0.80 -1.3 6,426,891 + Pf6N2E2_5611 0.84 -2.2 6,426,891 + Pf6N2E2_5611 0.84 +0.3 6,426,891 + Pf6N2E2_5611 0.84 -3.0 6,426,891 + Pf6N2E2_5611 0.84 -1.9 6,426,891 + Pf6N2E2_5611 0.84 -2.4 6,426,891 + Pf6N2E2_5611 0.84 -2.3 6,426,892 - Pf6N2E2_5611 0.84 -0.8 6,426,892 - Pf6N2E2_5611 0.84 +1.4 6,426,892 - Pf6N2E2_5611 0.84 -1.2 6,426,892 - Pf6N2E2_5611 0.84 +0.9 6,426,892 - Pf6N2E2_5611 0.84 -0.2 6,426,895 + Pf6N2E2_5611 0.85 -1.7 6,426,896 + Pf6N2E2_5611 0.85 -0.6 6,426,896 + Pf6N2E2_5611 0.85 -0.5 6,426,896 + Pf6N2E2_5611 0.85 -0.9 6,426,896 + Pf6N2E2_5611 0.85 -1.1 6,426,897 - Pf6N2E2_5611 0.85 -1.2 6,426,897 - Pf6N2E2_5611 0.85 -1.2 6,426,897 - Pf6N2E2_5611 0.85 -0.3 6,426,959 - +0.4 6,426,959 - -0.2 6,426,976 + +0.0 6,426,976 + -0.0 6,426,976 + -0.1 6,426,976 + -2.7 6,426,976 + +2.1 6,426,976 + -1.2 6,426,976 + +0.2 6,426,976 + -1.3 6,426,976 + -0.2 6,426,977 - -0.4 6,426,977 - +1.3 6,426,977 - -0.2 6,426,980 + +0.3 6,426,980 + +0.6 6,426,980 + +0.0 6,426,995 - +0.3 6,427,008 + -2.9 6,427,008 + -0.7 6,427,090 + Pf6N2E2_5612 0.13 +0.4 6,427,090 + Pf6N2E2_5612 0.13 -0.4 6,427,090 + Pf6N2E2_5612 0.13 -0.1 6,427,090 + Pf6N2E2_5612 0.13 +1.1 6,427,090 + Pf6N2E2_5612 0.13 +0.0 6,427,090 + Pf6N2E2_5612 0.13 -0.3 6,427,090 + Pf6N2E2_5612 0.13 +0.8 6,427,090 + Pf6N2E2_5612 0.13 -1.7 6,427,090 + Pf6N2E2_5612 0.13 +0.5 6,427,090 + Pf6N2E2_5612 0.13 -0.7 6,427,091 - Pf6N2E2_5612 0.13 -0.3 6,427,096 - Pf6N2E2_5612 0.13 +0.1 6,427,154 + Pf6N2E2_5612 0.22 +0.0 6,427,154 + Pf6N2E2_5612 0.22 +1.5 6,427,155 - Pf6N2E2_5612 0.22 -1.7 6,427,177 + Pf6N2E2_5612 0.25 +0.7 6,427,177 + Pf6N2E2_5612 0.25 +3.6 6,427,177 + Pf6N2E2_5612 0.25 -0.2 6,427,177 + Pf6N2E2_5612 0.25 +0.7 6,427,177 + Pf6N2E2_5612 0.25 +0.3 6,427,177 + Pf6N2E2_5612 0.25 +0.1 6,427,177 + Pf6N2E2_5612 0.25 -1.7 6,427,177 + Pf6N2E2_5612 0.25 +1.0 6,427,177 + Pf6N2E2_5612 0.25 +1.1 6,427,177 + Pf6N2E2_5612 0.25 -0.4 6,427,178 - Pf6N2E2_5612 0.25 -1.7 6,427,178 - Pf6N2E2_5612 0.25 +0.1 6,427,181 + Pf6N2E2_5612 0.25 +1.5 6,427,181 + Pf6N2E2_5612 0.25 -1.6 6,427,373 + Pf6N2E2_5612 0.52 +0.6 6,427,374 - Pf6N2E2_5612 0.52 -2.1 6,427,483 + Pf6N2E2_5612 0.68 +0.9 6,427,483 + Pf6N2E2_5612 0.68 -0.7 6,427,483 + Pf6N2E2_5612 0.68 +1.0 6,427,483 + Pf6N2E2_5612 0.68 +0.9 6,427,483 + Pf6N2E2_5612 0.68 +0.5 6,427,483 + Pf6N2E2_5612 0.68 +0.4 6,427,487 + Pf6N2E2_5612 0.68 +0.8 6,427,487 + Pf6N2E2_5612 0.68 +0.7 6,427,487 + Pf6N2E2_5612 0.68 +1.0 6,427,487 + Pf6N2E2_5612 0.68 +0.5 6,427,488 - Pf6N2E2_5612 0.68 -1.5 6,427,488 - Pf6N2E2_5612 0.68 +0.4 6,427,488 - Pf6N2E2_5612 0.68 -0.2 6,427,488 - Pf6N2E2_5612 0.68 -3.5 6,427,746 - +0.0 6,427,834 + Pf6N2E2_5613 0.37 +1.6 6,427,834 + Pf6N2E2_5613 0.37 +0.1
Or see this region's nucleotide sequence