Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5597

Experiment: D-Cycloserine 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5595 and Pf6N2E2_5596 are separated by 37 nucleotidesPf6N2E2_5596 and Pf6N2E2_5597 overlap by 4 nucleotidesPf6N2E2_5597 and Pf6N2E2_5598 overlap by 14 nucleotides Pf6N2E2_5595 - Electron transport complex protein RnfE, at 6,411,344 to 6,411,832 _5595 Pf6N2E2_5596 - Electron transport complex protein RnfG, at 6,411,870 to 6,412,481 _5596 Pf6N2E2_5597 - Electron transport complex protein RnfD, at 6,412,478 to 6,413,464 _5597 Pf6N2E2_5598 - Electron transport complex protein RnfB, at 6,413,451 to 6,414,470 _5598 Position (kb) 6412 6413 6414Strain fitness (log2 ratio) -3 -2 -1 0 1at 6411.612 kb on + strand, within Pf6N2E2_5595at 6411.613 kb on - strand, within Pf6N2E2_5595at 6411.613 kb on - strand, within Pf6N2E2_5595at 6411.631 kb on - strand, within Pf6N2E2_5595at 6411.631 kb on - strand, within Pf6N2E2_5595at 6411.631 kb on - strand, within Pf6N2E2_5595at 6411.697 kb on + strand, within Pf6N2E2_5595at 6411.698 kb on - strand, within Pf6N2E2_5595at 6411.792 kb on - strandat 6411.871 kb on + strandat 6411.871 kb on + strandat 6411.871 kb on + strandat 6411.871 kb on + strandat 6411.871 kb on + strandat 6411.872 kb on - strandat 6411.872 kb on - strandat 6411.872 kb on - strandat 6411.872 kb on - strandat 6411.872 kb on - strandat 6411.872 kb on - strandat 6411.872 kb on - strandat 6411.919 kb on + strandat 6411.920 kb on - strandat 6412.046 kb on + strand, within Pf6N2E2_5596at 6412.268 kb on + strand, within Pf6N2E2_5596at 6412.319 kb on + strand, within Pf6N2E2_5596at 6412.324 kb on + strand, within Pf6N2E2_5596at 6412.324 kb on + strand, within Pf6N2E2_5596at 6412.324 kb on + strand, within Pf6N2E2_5596at 6412.324 kb on + strand, within Pf6N2E2_5596at 6412.324 kb on + strand, within Pf6N2E2_5596at 6412.324 kb on + strand, within Pf6N2E2_5596at 6412.418 kb on - strand, within Pf6N2E2_5596at 6412.418 kb on - strand, within Pf6N2E2_5596at 6412.592 kb on + strand, within Pf6N2E2_5597at 6412.593 kb on - strand, within Pf6N2E2_5597at 6412.599 kb on + strand, within Pf6N2E2_5597at 6412.600 kb on - strand, within Pf6N2E2_5597at 6412.781 kb on + strand, within Pf6N2E2_5597at 6413.012 kb on + strand, within Pf6N2E2_5597at 6413.091 kb on - strand, within Pf6N2E2_5597at 6413.091 kb on - strand, within Pf6N2E2_5597at 6413.208 kb on + strand, within Pf6N2E2_5597at 6413.208 kb on + strand, within Pf6N2E2_5597at 6413.209 kb on - strand, within Pf6N2E2_5597at 6413.535 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml
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6,411,612 + Pf6N2E2_5595 0.55 -2.7
6,411,613 - Pf6N2E2_5595 0.55 +0.8
6,411,613 - Pf6N2E2_5595 0.55 -0.5
6,411,631 - Pf6N2E2_5595 0.59 +0.8
6,411,631 - Pf6N2E2_5595 0.59 +1.2
6,411,631 - Pf6N2E2_5595 0.59 +0.7
6,411,697 + Pf6N2E2_5595 0.72 -0.1
6,411,698 - Pf6N2E2_5595 0.72 +0.1
6,411,792 - +0.8
6,411,871 + -2.9
6,411,871 + +0.1
6,411,871 + -2.8
6,411,871 + -2.8
6,411,871 + -0.6
6,411,872 - -0.4
6,411,872 - +0.1
6,411,872 - +0.4
6,411,872 - +0.8
6,411,872 - +0.8
6,411,872 - -0.4
6,411,872 - -0.7
6,411,919 + -1.6
6,411,920 - +0.1
6,412,046 + Pf6N2E2_5596 0.29 -0.9
6,412,268 + Pf6N2E2_5596 0.65 -0.8
6,412,319 + Pf6N2E2_5596 0.73 -0.8
6,412,324 + Pf6N2E2_5596 0.74 -0.2
6,412,324 + Pf6N2E2_5596 0.74 -0.1
6,412,324 + Pf6N2E2_5596 0.74 -0.9
6,412,324 + Pf6N2E2_5596 0.74 -0.3
6,412,324 + Pf6N2E2_5596 0.74 -0.9
6,412,324 + Pf6N2E2_5596 0.74 -0.7
6,412,418 - Pf6N2E2_5596 0.90 +0.9
6,412,418 - Pf6N2E2_5596 0.90 +0.3
6,412,592 + Pf6N2E2_5597 0.12 +0.1
6,412,593 - Pf6N2E2_5597 0.12 -0.1
6,412,599 + Pf6N2E2_5597 0.12 -2.4
6,412,600 - Pf6N2E2_5597 0.12 -0.3
6,412,781 + Pf6N2E2_5597 0.31 -1.9
6,413,012 + Pf6N2E2_5597 0.54 -0.6
6,413,091 - Pf6N2E2_5597 0.62 +0.8
6,413,091 - Pf6N2E2_5597 0.62 +0.1
6,413,208 + Pf6N2E2_5597 0.74 -1.1
6,413,208 + Pf6N2E2_5597 0.74 -2.6
6,413,209 - Pf6N2E2_5597 0.74 -0.0
6,413,535 - +0.5

Or see this region's nucleotide sequence