Experiment: D-Cycloserine 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4398 and Pf6N2E2_4399 are separated by 273 nucleotides Pf6N2E2_4399 and Pf6N2E2_4400 are separated by 48 nucleotides
Pf6N2E2_4398 - ATP-dependent DNA helicase Rep, at 5,126,346 to 5,128,355
_4398
Pf6N2E2_4399 - GGDEF domain/EAL domain protein, at 5,128,629 to 5,130,299
_4399
Pf6N2E2_4400 - Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog, at 5,130,348 to 5,131,748
_4400
Position (kb)
5128
5129
5130
5131 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5128.396 kb on + strand at 5128.468 kb on + strand at 5128.468 kb on + strand at 5128.468 kb on + strand at 5128.468 kb on + strand at 5128.468 kb on + strand at 5128.469 kb on - strand at 5128.586 kb on + strand at 5128.586 kb on + strand at 5128.586 kb on + strand at 5128.587 kb on - strand at 5128.587 kb on - strand at 5128.601 kb on - strand at 5128.617 kb on + strand at 5128.617 kb on + strand at 5128.664 kb on - strand at 5128.664 kb on - strand at 5128.694 kb on - strand at 5128.694 kb on - strand at 5128.781 kb on + strand at 5128.781 kb on + strand at 5128.782 kb on - strand at 5129.300 kb on + strand, within Pf6N2E2_4399 at 5129.300 kb on + strand, within Pf6N2E2_4399 at 5129.300 kb on + strand, within Pf6N2E2_4399 at 5129.300 kb on + strand, within Pf6N2E2_4399 at 5129.301 kb on - strand, within Pf6N2E2_4399 at 5129.325 kb on + strand, within Pf6N2E2_4399 at 5129.326 kb on - strand, within Pf6N2E2_4399 at 5129.468 kb on + strand, within Pf6N2E2_4399 at 5129.468 kb on + strand, within Pf6N2E2_4399 at 5129.468 kb on + strand, within Pf6N2E2_4399 at 5129.469 kb on - strand, within Pf6N2E2_4399 at 5129.469 kb on - strand, within Pf6N2E2_4399 at 5129.469 kb on - strand, within Pf6N2E2_4399 at 5129.469 kb on - strand, within Pf6N2E2_4399 at 5129.565 kb on + strand, within Pf6N2E2_4399 at 5129.620 kb on + strand, within Pf6N2E2_4399 at 5129.620 kb on + strand, within Pf6N2E2_4399 at 5129.710 kb on - strand, within Pf6N2E2_4399 at 5130.178 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.252 kb on + strand at 5130.253 kb on - strand at 5130.253 kb on - strand at 5130.253 kb on - strand at 5130.253 kb on - strand at 5130.253 kb on - strand at 5130.253 kb on - strand at 5130.544 kb on + strand, within Pf6N2E2_4400 at 5130.545 kb on - strand, within Pf6N2E2_4400 at 5130.836 kb on - strand, within Pf6N2E2_4400 at 5130.836 kb on - strand, within Pf6N2E2_4400 at 5130.865 kb on + strand, within Pf6N2E2_4400 at 5130.866 kb on - strand, within Pf6N2E2_4400 at 5130.997 kb on + strand, within Pf6N2E2_4400 at 5130.997 kb on + strand, within Pf6N2E2_4400 at 5130.997 kb on + strand, within Pf6N2E2_4400 at 5130.998 kb on - strand, within Pf6N2E2_4400 at 5130.998 kb on - strand, within Pf6N2E2_4400 at 5131.091 kb on - strand, within Pf6N2E2_4400 at 5131.109 kb on + strand, within Pf6N2E2_4400 at 5131.110 kb on - strand, within Pf6N2E2_4400 at 5131.110 kb on - strand, within Pf6N2E2_4400 at 5131.216 kb on + strand, within Pf6N2E2_4400 at 5131.216 kb on + strand, within Pf6N2E2_4400 at 5131.216 kb on + strand, within Pf6N2E2_4400 at 5131.216 kb on + strand, within Pf6N2E2_4400 at 5131.217 kb on - strand, within Pf6N2E2_4400
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.12 mg/ml remove 5,128,396 + -0.6 5,128,468 + +0.5 5,128,468 + +0.8 5,128,468 + -0.4 5,128,468 + -1.0 5,128,468 + -0.2 5,128,469 - +1.3 5,128,586 + +0.2 5,128,586 + +0.2 5,128,586 + -1.4 5,128,587 - -0.7 5,128,587 - -0.0 5,128,601 - -0.7 5,128,617 + +0.8 5,128,617 + -0.8 5,128,664 - -0.3 5,128,664 - +1.5 5,128,694 - -0.5 5,128,694 - +0.5 5,128,781 + -1.1 5,128,781 + +0.2 5,128,782 - +0.6 5,129,300 + Pf6N2E2_4399 0.40 -1.1 5,129,300 + Pf6N2E2_4399 0.40 -0.8 5,129,300 + Pf6N2E2_4399 0.40 -0.1 5,129,300 + Pf6N2E2_4399 0.40 +1.1 5,129,301 - Pf6N2E2_4399 0.40 -1.6 5,129,325 + Pf6N2E2_4399 0.42 +1.1 5,129,326 - Pf6N2E2_4399 0.42 +1.2 5,129,468 + Pf6N2E2_4399 0.50 +0.2 5,129,468 + Pf6N2E2_4399 0.50 +2.1 5,129,468 + Pf6N2E2_4399 0.50 -0.5 5,129,469 - Pf6N2E2_4399 0.50 +1.0 5,129,469 - Pf6N2E2_4399 0.50 -2.9 5,129,469 - Pf6N2E2_4399 0.50 -1.2 5,129,469 - Pf6N2E2_4399 0.50 +0.7 5,129,565 + Pf6N2E2_4399 0.56 -0.5 5,129,620 + Pf6N2E2_4399 0.59 +0.3 5,129,620 + Pf6N2E2_4399 0.59 -1.1 5,129,710 - Pf6N2E2_4399 0.65 +1.8 5,130,178 + -2.8 5,130,252 + +0.7 5,130,252 + +0.8 5,130,252 + +0.1 5,130,252 + +0.2 5,130,252 + -1.1 5,130,252 + +0.4 5,130,252 + +0.5 5,130,252 + -2.5 5,130,253 - +0.4 5,130,253 - +0.8 5,130,253 - -0.5 5,130,253 - -0.3 5,130,253 - -0.4 5,130,253 - -0.1 5,130,544 + Pf6N2E2_4400 0.14 +0.6 5,130,545 - Pf6N2E2_4400 0.14 -0.6 5,130,836 - Pf6N2E2_4400 0.35 +0.6 5,130,836 - Pf6N2E2_4400 0.35 +0.6 5,130,865 + Pf6N2E2_4400 0.37 +0.4 5,130,866 - Pf6N2E2_4400 0.37 -0.1 5,130,997 + Pf6N2E2_4400 0.46 -0.3 5,130,997 + Pf6N2E2_4400 0.46 +0.3 5,130,997 + Pf6N2E2_4400 0.46 +0.1 5,130,998 - Pf6N2E2_4400 0.46 -1.0 5,130,998 - Pf6N2E2_4400 0.46 +0.8 5,131,091 - Pf6N2E2_4400 0.53 +0.7 5,131,109 + Pf6N2E2_4400 0.54 -0.9 5,131,110 - Pf6N2E2_4400 0.54 -0.1 5,131,110 - Pf6N2E2_4400 0.54 -0.1 5,131,216 + Pf6N2E2_4400 0.62 +0.8 5,131,216 + Pf6N2E2_4400 0.62 +1.5 5,131,216 + Pf6N2E2_4400 0.62 -0.4 5,131,216 + Pf6N2E2_4400 0.62 +0.7 5,131,217 - Pf6N2E2_4400 0.62 +1.1
Or see this region's nucleotide sequence