Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF772 and GFF773 are separated by 229 nucleotides GFF773 and GFF774 are separated by 95 nucleotides GFF774 and GFF775 are separated by 54 nucleotides GFF775 and GFF776 are separated by 7 nucleotides GFF776 and GFF777 are separated by 205 nucleotides
GFF772 - Arginine ABC transporter, ATP-binding protein ArtP, at 128,107 to 128,835
GFF772
GFF773 - probable lipoprotein, at 129,065 to 129,580
GFF773
GFF774 - putative integrase, at 129,676 to 130,974
GFF774
GFF775 - putative excisionase, at 131,029 to 131,271
GFF775
GFF776 - Phage protein, at 131,279 to 131,431
GFF776
GFF777 - COG0419: ATPase involved in DNA repair, at 131,637 to 131,945
GFF777
Position (kb) 129
130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 128.845 kb on + strand at 128.846 kb on - strand at 128.865 kb on + strand at 128.865 kb on + strand at 128.866 kb on - strand at 128.866 kb on - strand at 128.880 kb on + strand at 128.955 kb on + strand at 128.981 kb on - strand at 129.169 kb on + strand, within GFF773 at 129.569 kb on - strand at 129.569 kb on - strand at 129.593 kb on - strand at 129.594 kb on + strand at 129.597 kb on - strand at 129.619 kb on - strand at 129.724 kb on - strand at 129.746 kb on - strand at 129.751 kb on + strand at 129.752 kb on - strand at 129.752 kb on - strand at 129.752 kb on - strand at 129.790 kb on + strand at 129.795 kb on + strand at 129.796 kb on - strand at 129.796 kb on - strand at 129.796 kb on - strand at 129.878 kb on + strand, within GFF774 at 129.969 kb on - strand, within GFF774 at 130.042 kb on - strand, within GFF774 at 130.050 kb on - strand, within GFF774 at 130.063 kb on + strand, within GFF774 at 130.144 kb on + strand, within GFF774 at 130.145 kb on - strand, within GFF774 at 130.265 kb on - strand, within GFF774 at 130.282 kb on - strand, within GFF774 at 130.402 kb on - strand, within GFF774 at 130.402 kb on - strand, within GFF774 at 130.402 kb on - strand, within GFF774 at 130.542 kb on + strand, within GFF774 at 130.543 kb on - strand, within GFF774 at 130.800 kb on + strand, within GFF774 at 130.801 kb on - strand, within GFF774 at 130.801 kb on - strand, within GFF774 at 130.801 kb on - strand, within GFF774 at 130.920 kb on + strand at 130.974 kb on + strand at 131.055 kb on + strand, within GFF775 at 131.090 kb on + strand, within GFF775 at 131.091 kb on - strand, within GFF775 at 131.139 kb on + strand, within GFF775 at 131.139 kb on + strand, within GFF775 at 131.140 kb on - strand, within GFF775 at 131.140 kb on - strand, within GFF775 at 131.249 kb on - strand at 131.255 kb on + strand at 131.260 kb on - strand at 131.467 kb on + strand at 131.469 kb on + strand at 131.470 kb on - strand at 131.483 kb on + strand at 131.584 kb on - strand at 131.639 kb on - strand at 131.639 kb on - strand at 131.708 kb on - strand, within GFF777 at 131.839 kb on + strand, within GFF777 at 131.910 kb on + strand, within GFF777 at 131.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 128,845 + +0.7 128,846 - +0.5 128,865 + -0.2 128,865 + -0.6 128,866 - +1.5 128,866 - -2.8 128,880 + -1.4 128,955 + +0.7 128,981 - +0.4 129,169 + GFF773 0.20 -0.1 129,569 - -0.8 129,569 - +0.7 129,593 - +0.5 129,594 + +0.5 129,597 - +0.9 129,619 - -3.0 129,724 - +2.2 129,746 - +0.3 129,751 + -0.9 129,752 - +0.4 129,752 - +0.4 129,752 - +1.0 129,790 + +1.0 129,795 + +0.6 129,796 - -0.1 129,796 - +0.6 129,796 - +0.4 129,878 + GFF774 0.16 +1.1 129,969 - GFF774 0.23 -0.7 130,042 - GFF774 0.28 +0.9 130,050 - GFF774 0.29 -0.5 130,063 + GFF774 0.30 -0.4 130,144 + GFF774 0.36 -0.7 130,145 - GFF774 0.36 +2.4 130,265 - GFF774 0.45 +0.7 130,282 - GFF774 0.47 -1.2 130,402 - GFF774 0.56 +0.4 130,402 - GFF774 0.56 +0.2 130,402 - GFF774 0.56 +0.4 130,542 + GFF774 0.67 +1.1 130,543 - GFF774 0.67 +0.3 130,800 + GFF774 0.87 +0.6 130,801 - GFF774 0.87 +1.2 130,801 - GFF774 0.87 +1.3 130,801 - GFF774 0.87 +1.8 130,920 + +0.2 130,974 + -1.1 131,055 + GFF775 0.11 -1.6 131,090 + GFF775 0.25 +0.9 131,091 - GFF775 0.26 -0.6 131,139 + GFF775 0.45 -0.1 131,139 + GFF775 0.45 -2.1 131,140 - GFF775 0.46 +0.5 131,140 - GFF775 0.46 -0.9 131,249 - -1.1 131,255 + +0.7 131,260 - -1.6 131,467 + -0.5 131,469 + +0.0 131,470 - -0.4 131,483 + +0.0 131,584 - -0.1 131,639 - +0.4 131,639 - -0.7 131,708 - GFF777 0.23 +1.5 131,839 + GFF777 0.65 -0.9 131,910 + GFF777 0.88 -1.8 131,922 - +0.2
Or see this region's nucleotide sequence