Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3986 and GFF4036 are separated by 88 nucleotides GFF4036 and GFF3987 are separated by 302 nucleotides GFF3987 and GFF3988 are separated by 306 nucleotides GFF3988 and GFF3989 are separated by 2 nucleotides
GFF3986 - FIG01220476: hypothetical protein, at 261,705 to 262,502
GFF3986
GFF4036 - tRNA-Ser-CGA, at 262,591 to 262,680
GFF4036
GFF3987 - FIG01045245: hypothetical protein, at 262,983 to 263,144
GFF3987
GFF3988 - Error-prone repair protein UmuD, at 263,451 to 263,690
GFF3988
GFF3989 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 263,693 to 264,961
GFF3989
Position (kb) 263
264 Strain fitness (log2 ratio) -2
-1
0
1 at 262.568 kb on + strand at 262.637 kb on + strand, within GFF4036 at 262.842 kb on + strand at 262.842 kb on + strand at 262.978 kb on + strand at 262.978 kb on + strand at 262.979 kb on - strand at 262.979 kb on - strand at 263.004 kb on - strand, within GFF3987 at 263.004 kb on - strand, within GFF3987 at 263.044 kb on + strand, within GFF3987 at 263.044 kb on + strand, within GFF3987 at 263.198 kb on - strand at 263.496 kb on - strand, within GFF3988 at 263.496 kb on - strand, within GFF3988 at 263.528 kb on - strand, within GFF3988 at 263.590 kb on + strand, within GFF3988 at 263.621 kb on - strand, within GFF3988 at 263.670 kb on - strand at 263.670 kb on - strand at 263.713 kb on + strand at 263.812 kb on + strand at 263.813 kb on - strand at 263.856 kb on - strand, within GFF3989 at 263.913 kb on - strand, within GFF3989 at 264.102 kb on + strand, within GFF3989 at 264.268 kb on + strand, within GFF3989 at 264.268 kb on + strand, within GFF3989 at 264.323 kb on - strand, within GFF3989 at 264.471 kb on - strand, within GFF3989 at 264.495 kb on - strand, within GFF3989 at 264.659 kb on - strand, within GFF3989
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 262,568 + +0.5 262,637 + GFF4036 0.51 -0.4 262,842 + -0.5 262,842 + -0.3 262,978 + +0.2 262,978 + -0.0 262,979 - -0.2 262,979 - +0.0 263,004 - GFF3987 0.13 -0.8 263,004 - GFF3987 0.13 -2.7 263,044 + GFF3987 0.38 -0.0 263,044 + GFF3987 0.38 -0.2 263,198 - -0.8 263,496 - GFF3988 0.19 -0.6 263,496 - GFF3988 0.19 -0.7 263,528 - GFF3988 0.32 -1.7 263,590 + GFF3988 0.58 -0.1 263,621 - GFF3988 0.71 -1.2 263,670 - -0.1 263,670 - +0.0 263,713 + -0.3 263,812 + -0.8 263,813 - -0.1 263,856 - GFF3989 0.13 -0.4 263,913 - GFF3989 0.17 -0.3 264,102 + GFF3989 0.32 -0.9 264,268 + GFF3989 0.45 +0.1 264,268 + GFF3989 0.45 -0.3 264,323 - GFF3989 0.50 +0.7 264,471 - GFF3989 0.61 +1.1 264,495 - GFF3989 0.63 +0.1 264,659 - GFF3989 0.76 -1.9
Or see this region's nucleotide sequence