Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3634 and GFF3635 are separated by 93 nucleotides GFF3635 and GFF3636 are separated by 58 nucleotides GFF3636 and GFF3637 are separated by 126 nucleotides
GFF3634 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 383,160 to 384,281
GFF3634
GFF3635 - Membrane-bound lysozyme inhibitor of c-type lysozyme, at 384,375 to 384,698
GFF3635
GFF3636 - 'Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)' transl_table=11, at 384,757 to 385,413
GFF3636
GFF3637 - Tyrosyl-tRNA synthetase (EC 6.1.1.1), at 385,540 to 386,814
GFF3637
Position (kb) 384
385 Strain fitness (log2 ratio) -2
-1
0
1 at 383.493 kb on - strand, within GFF3634 at 383.513 kb on + strand, within GFF3634 at 383.648 kb on + strand, within GFF3634 at 383.648 kb on + strand, within GFF3634 at 383.735 kb on + strand, within GFF3634 at 383.736 kb on - strand, within GFF3634 at 383.736 kb on - strand, within GFF3634 at 383.901 kb on + strand, within GFF3634 at 383.901 kb on + strand, within GFF3634 at 383.902 kb on - strand, within GFF3634 at 383.902 kb on - strand, within GFF3634 at 383.911 kb on + strand, within GFF3634 at 383.956 kb on + strand, within GFF3634 at 384.098 kb on + strand, within GFF3634 at 384.119 kb on + strand, within GFF3634 at 384.120 kb on - strand, within GFF3634 at 384.143 kb on + strand, within GFF3634 at 384.314 kb on + strand at 384.462 kb on - strand, within GFF3635 at 384.591 kb on + strand, within GFF3635 at 384.594 kb on - strand, within GFF3635 at 384.666 kb on - strand at 384.668 kb on + strand at 385.468 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 383,493 - GFF3634 0.30 +0.2 383,513 + GFF3634 0.31 +0.0 383,648 + GFF3634 0.43 +0.3 383,648 + GFF3634 0.43 -0.4 383,735 + GFF3634 0.51 +0.0 383,736 - GFF3634 0.51 -0.1 383,736 - GFF3634 0.51 -1.2 383,901 + GFF3634 0.66 -0.5 383,901 + GFF3634 0.66 -1.3 383,902 - GFF3634 0.66 +0.5 383,902 - GFF3634 0.66 -0.5 383,911 + GFF3634 0.67 -1.0 383,956 + GFF3634 0.71 -0.8 384,098 + GFF3634 0.84 -0.3 384,119 + GFF3634 0.85 -0.4 384,120 - GFF3634 0.86 -0.7 384,143 + GFF3634 0.88 -0.7 384,314 + +0.4 384,462 - GFF3635 0.27 -1.2 384,591 + GFF3635 0.67 -2.4 384,594 - GFF3635 0.68 +0.3 384,666 - -0.2 384,668 + -0.2 385,468 + +1.1
Or see this region's nucleotide sequence