Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4741 and Pf1N1B4_4742 overlap by 4 nucleotides Pf1N1B4_4742 and Pf1N1B4_4743 are separated by 6 nucleotides Pf1N1B4_4743 and Pf1N1B4_4744 overlap by 4 nucleotides Pf1N1B4_4744 and Pf1N1B4_4745 are separated by 14 nucleotides Pf1N1B4_4745 and Pf1N1B4_4746 are separated by 132 nucleotides
Pf1N1B4_4741 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 5,238,743 to 5,239,573
_4741
Pf1N1B4_4742 - Cold-shock DNA-binding domain, at 5,239,570 to 5,240,001
_4742
Pf1N1B4_4743 - FIG00955061: hypothetical protein, at 5,240,008 to 5,240,367
_4743
Pf1N1B4_4744 - Transcriptional regulator, LysR family, at 5,240,364 to 5,240,957
_4744
Pf1N1B4_4745 - Transcriptional regulator, LysR family, at 5,240,972 to 5,241,271
_4745
Pf1N1B4_4746 - FMN-dependent NADH-azoreductase, at 5,241,404 to 5,242,015
_4746
Position (kb) 5240
5241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5239.459 kb on - strand, within Pf1N1B4_4741 at 5240.017 kb on + strand at 5240.017 kb on + strand at 5240.294 kb on + strand, within Pf1N1B4_4743 at 5240.295 kb on - strand, within Pf1N1B4_4743 at 5240.295 kb on - strand, within Pf1N1B4_4743 at 5240.499 kb on + strand, within Pf1N1B4_4744 at 5240.499 kb on + strand, within Pf1N1B4_4744 at 5240.500 kb on - strand, within Pf1N1B4_4744 at 5240.539 kb on + strand, within Pf1N1B4_4744 at 5240.539 kb on + strand, within Pf1N1B4_4744 at 5240.539 kb on + strand, within Pf1N1B4_4744 at 5240.558 kb on + strand, within Pf1N1B4_4744 at 5240.559 kb on - strand, within Pf1N1B4_4744 at 5240.559 kb on - strand, within Pf1N1B4_4744 at 5241.078 kb on + strand, within Pf1N1B4_4745 at 5241.317 kb on + strand at 5241.412 kb on - strand at 5241.412 kb on - strand at 5241.690 kb on - strand, within Pf1N1B4_4746 at 5241.761 kb on + strand at 5241.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 5,239,459 - Pf1N1B4_4741 0.86 -0.6 5,240,017 + -0.7 5,240,017 + +2.4 5,240,294 + Pf1N1B4_4743 0.79 -0.0 5,240,295 - Pf1N1B4_4743 0.80 -0.3 5,240,295 - Pf1N1B4_4743 0.80 +0.4 5,240,499 + Pf1N1B4_4744 0.23 +0.4 5,240,499 + Pf1N1B4_4744 0.23 -1.6 5,240,500 - Pf1N1B4_4744 0.23 -1.4 5,240,539 + Pf1N1B4_4744 0.29 -0.6 5,240,539 + Pf1N1B4_4744 0.29 -1.2 5,240,539 + Pf1N1B4_4744 0.29 +1.0 5,240,558 + Pf1N1B4_4744 0.33 -0.6 5,240,559 - Pf1N1B4_4744 0.33 -2.8 5,240,559 - Pf1N1B4_4744 0.33 -2.9 5,241,078 + Pf1N1B4_4745 0.35 +0.4 5,241,317 + +1.7 5,241,412 - +0.4 5,241,412 - +0.4 5,241,690 - Pf1N1B4_4746 0.47 +0.4 5,241,761 + +0.4 5,241,870 - -0.4
Or see this region's nucleotide sequence