Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3970 and Pf1N1B4_3971 are separated by 154 nucleotides Pf1N1B4_3971 and Pf1N1B4_3972 are separated by 8 nucleotides Pf1N1B4_3972 and Pf1N1B4_3973 are separated by 6 nucleotides Pf1N1B4_3973 and Pf1N1B4_3974 overlap by 4 nucleotides
Pf1N1B4_3970 - Ferredoxin reductase, at 4,411,553 to 4,412,908
_3970
Pf1N1B4_3971 - Opine oxidase subunit C, at 4,413,063 to 4,413,353
_3971
Pf1N1B4_3972 - ABC-type spermidine/putrescine transport system, permease component II, at 4,413,362 to 4,414,156
_3972
Pf1N1B4_3973 - ABC-type spermidine/putrescine transport system, permease component I, at 4,414,163 to 4,415,032
_3973
Pf1N1B4_3974 - ABC transporter, ATP-binding protein, at 4,415,029 to 4,416,054
_3974
Position (kb)
4413
4414
4415 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4412.517 kb on + strand, within Pf1N1B4_3970 at 4412.517 kb on + strand at 4412.517 kb on + strand at 4412.517 kb on + strand, within Pf1N1B4_3970 at 4412.517 kb on + strand, within Pf1N1B4_3970 at 4412.517 kb on + strand, within Pf1N1B4_3970 at 4412.518 kb on - strand, within Pf1N1B4_3970 at 4412.518 kb on - strand, within Pf1N1B4_3970 at 4412.518 kb on - strand, within Pf1N1B4_3970 at 4412.742 kb on + strand, within Pf1N1B4_3970 at 4412.742 kb on + strand, within Pf1N1B4_3970 at 4412.742 kb on + strand, within Pf1N1B4_3970 at 4412.742 kb on + strand, within Pf1N1B4_3970 at 4412.743 kb on - strand, within Pf1N1B4_3970 at 4412.787 kb on + strand at 4412.787 kb on + strand at 4412.908 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.919 kb on + strand at 4412.920 kb on - strand at 4412.920 kb on - strand at 4412.920 kb on - strand at 4412.920 kb on - strand at 4412.920 kb on - strand at 4412.920 kb on - strand at 4412.920 kb on - strand at 4412.982 kb on + strand at 4412.982 kb on + strand at 4412.982 kb on + strand at 4412.982 kb on + strand at 4412.983 kb on - strand at 4412.983 kb on - strand at 4413.066 kb on + strand at 4413.067 kb on - strand at 4413.067 kb on - strand at 4413.067 kb on - strand at 4413.067 kb on - strand at 4413.489 kb on + strand, within Pf1N1B4_3972 at 4413.490 kb on - strand, within Pf1N1B4_3972 at 4413.495 kb on + strand at 4413.495 kb on + strand, within Pf1N1B4_3972 at 4413.495 kb on + strand at 4413.496 kb on - strand, within Pf1N1B4_3972 at 4413.496 kb on - strand, within Pf1N1B4_3972 at 4413.727 kb on - strand, within Pf1N1B4_3972 at 4413.727 kb on - strand, within Pf1N1B4_3972 at 4414.079 kb on + strand at 4414.287 kb on + strand, within Pf1N1B4_3973 at 4414.287 kb on + strand, within Pf1N1B4_3973 at 4414.575 kb on + strand at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.828 kb on - strand, within Pf1N1B4_3973 at 4414.833 kb on + strand, within Pf1N1B4_3973 at 4414.834 kb on - strand, within Pf1N1B4_3973 at 4414.886 kb on - strand at 4414.886 kb on - strand at 4414.886 kb on - strand at 4414.963 kb on - strand at 4414.963 kb on - strand at 4414.963 kb on - strand at 4415.026 kb on + strand at 4415.026 kb on + strand at 4415.026 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 4,412,517 + Pf1N1B4_3970 0.71 -1.1 4,412,517 + -0.6 4,412,517 + +0.7 4,412,517 + Pf1N1B4_3970 0.71 -0.6 4,412,517 + Pf1N1B4_3970 0.71 +0.7 4,412,517 + Pf1N1B4_3970 0.71 +0.1 4,412,518 - Pf1N1B4_3970 0.71 +3.3 4,412,518 - Pf1N1B4_3970 0.71 +0.7 4,412,518 - Pf1N1B4_3970 0.71 +0.7 4,412,742 + Pf1N1B4_3970 0.88 -0.8 4,412,742 + Pf1N1B4_3970 0.88 -0.0 4,412,742 + Pf1N1B4_3970 0.88 +1.1 4,412,742 + Pf1N1B4_3970 0.88 -0.9 4,412,743 - Pf1N1B4_3970 0.88 +0.7 4,412,787 + +0.4 4,412,787 + -0.5 4,412,908 + +0.7 4,412,919 + -0.9 4,412,919 + -1.0 4,412,919 + +0.3 4,412,919 + -0.0 4,412,919 + +2.0 4,412,919 + +0.8 4,412,919 + -2.3 4,412,919 + -1.6 4,412,919 + +0.1 4,412,919 + +1.7 4,412,920 - +0.1 4,412,920 - +0.3 4,412,920 - -0.3 4,412,920 - +0.7 4,412,920 - -1.8 4,412,920 - -1.0 4,412,920 - -0.1 4,412,982 + +0.1 4,412,982 + -0.0 4,412,982 + -0.9 4,412,982 + +0.1 4,412,983 - -2.3 4,412,983 - +0.1 4,413,066 + -0.9 4,413,067 - -0.2 4,413,067 - -0.6 4,413,067 - -0.3 4,413,067 - +0.0 4,413,489 + Pf1N1B4_3972 0.16 -1.9 4,413,490 - Pf1N1B4_3972 0.16 -0.5 4,413,495 + +1.7 4,413,495 + Pf1N1B4_3972 0.17 -0.9 4,413,495 + -0.3 4,413,496 - Pf1N1B4_3972 0.17 -1.6 4,413,496 - Pf1N1B4_3972 0.17 -2.9 4,413,727 - Pf1N1B4_3972 0.46 -0.4 4,413,727 - Pf1N1B4_3972 0.46 +0.1 4,414,079 + +1.3 4,414,287 + Pf1N1B4_3973 0.14 +0.7 4,414,287 + Pf1N1B4_3973 0.14 -1.0 4,414,575 + +0.3 4,414,749 + Pf1N1B4_3973 0.67 +0.2 4,414,749 + Pf1N1B4_3973 0.67 -0.9 4,414,749 + Pf1N1B4_3973 0.67 -1.6 4,414,749 + Pf1N1B4_3973 0.67 +1.1 4,414,828 - Pf1N1B4_3973 0.76 +0.7 4,414,833 + Pf1N1B4_3973 0.77 -1.5 4,414,834 - Pf1N1B4_3973 0.77 +0.1 4,414,886 - -1.6 4,414,886 - +1.3 4,414,886 - +1.7 4,414,963 - -0.3 4,414,963 - +0.7 4,414,963 - -2.3 4,415,026 + -0.3 4,415,026 + -1.0 4,415,026 + -1.5
Or see this region's nucleotide sequence