Experiment: Nalidixic 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3363 and Pf1N1B4_3364 are separated by 123 nucleotides Pf1N1B4_3364 and Pf1N1B4_3365 are separated by 147 nucleotides
Pf1N1B4_3363 - Siderophore synthetase superfamily, group A @ Siderophore synthetase large component, acetyltransferase, at 3,749,902 to 3,751,824
_3363
Pf1N1B4_3364 - Cyn operon transcriptional activator, at 3,751,948 to 3,752,865
_3364
Pf1N1B4_3365 - Sialic acid transporter (permease) NanT, at 3,753,013 to 3,754,296
_3365
Position (kb)
3751
3752
3753 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3750.983 kb on + strand, within Pf1N1B4_3363 at 3750.983 kb on + strand, within Pf1N1B4_3363 at 3751.106 kb on + strand, within Pf1N1B4_3363 at 3751.107 kb on - strand, within Pf1N1B4_3363 at 3751.205 kb on + strand, within Pf1N1B4_3363 at 3751.205 kb on + strand, within Pf1N1B4_3363 at 3751.205 kb on + strand, within Pf1N1B4_3363 at 3751.205 kb on + strand, within Pf1N1B4_3363 at 3751.205 kb on + strand, within Pf1N1B4_3363 at 3751.205 kb on + strand, within Pf1N1B4_3363 at 3751.206 kb on - strand, within Pf1N1B4_3363 at 3751.206 kb on - strand, within Pf1N1B4_3363 at 3751.206 kb on - strand at 3751.311 kb on - strand, within Pf1N1B4_3363 at 3751.420 kb on + strand, within Pf1N1B4_3363 at 3751.421 kb on - strand, within Pf1N1B4_3363 at 3751.535 kb on - strand at 3751.629 kb on - strand, within Pf1N1B4_3363 at 3751.966 kb on + strand at 3751.966 kb on + strand at 3751.967 kb on - strand at 3751.970 kb on + strand at 3751.970 kb on + strand at 3751.970 kb on + strand at 3751.971 kb on - strand at 3752.182 kb on + strand, within Pf1N1B4_3364 at 3752.183 kb on - strand, within Pf1N1B4_3364 at 3752.295 kb on + strand, within Pf1N1B4_3364 at 3752.295 kb on + strand, within Pf1N1B4_3364 at 3752.334 kb on - strand, within Pf1N1B4_3364 at 3752.334 kb on - strand, within Pf1N1B4_3364 at 3752.345 kb on + strand, within Pf1N1B4_3364 at 3752.882 kb on + strand at 3752.882 kb on + strand at 3752.904 kb on + strand at 3752.904 kb on + strand at 3752.984 kb on + strand at 3752.984 kb on + strand at 3752.984 kb on + strand at 3753.074 kb on - strand at 3753.102 kb on + strand at 3753.102 kb on + strand at 3753.364 kb on + strand, within Pf1N1B4_3365 at 3753.364 kb on + strand, within Pf1N1B4_3365 at 3753.364 kb on + strand at 3753.365 kb on - strand, within Pf1N1B4_3365 at 3753.365 kb on - strand, within Pf1N1B4_3365 at 3753.669 kb on + strand, within Pf1N1B4_3365 at 3753.669 kb on + strand, within Pf1N1B4_3365 at 3753.778 kb on + strand, within Pf1N1B4_3365 at 3753.778 kb on + strand, within Pf1N1B4_3365 at 3753.833 kb on + strand, within Pf1N1B4_3365 at 3753.834 kb on - strand, within Pf1N1B4_3365
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.004 mg/ml remove 3,750,983 + Pf1N1B4_3363 0.56 -1.4 3,750,983 + Pf1N1B4_3363 0.56 -1.3 3,751,106 + Pf1N1B4_3363 0.63 -1.7 3,751,107 - Pf1N1B4_3363 0.63 -0.6 3,751,205 + Pf1N1B4_3363 0.68 -1.7 3,751,205 + Pf1N1B4_3363 0.68 -2.1 3,751,205 + Pf1N1B4_3363 0.68 -1.7 3,751,205 + Pf1N1B4_3363 0.68 +1.9 3,751,205 + Pf1N1B4_3363 0.68 -0.8 3,751,205 + Pf1N1B4_3363 0.68 +0.9 3,751,206 - Pf1N1B4_3363 0.68 +1.2 3,751,206 - Pf1N1B4_3363 0.68 -1.2 3,751,206 - +1.3 3,751,311 - Pf1N1B4_3363 0.73 -1.8 3,751,420 + Pf1N1B4_3363 0.79 -0.5 3,751,421 - Pf1N1B4_3363 0.79 -0.9 3,751,535 - +2.5 3,751,629 - Pf1N1B4_3363 0.90 -0.5 3,751,966 + +0.4 3,751,966 + -2.3 3,751,967 - -0.7 3,751,970 + +2.5 3,751,970 + -0.1 3,751,970 + +1.9 3,751,971 - -0.1 3,752,182 + Pf1N1B4_3364 0.25 -1.9 3,752,183 - Pf1N1B4_3364 0.26 -2.9 3,752,295 + Pf1N1B4_3364 0.38 +0.6 3,752,295 + Pf1N1B4_3364 0.38 -1.7 3,752,334 - Pf1N1B4_3364 0.42 +0.9 3,752,334 - Pf1N1B4_3364 0.42 -1.2 3,752,345 + Pf1N1B4_3364 0.43 -1.4 3,752,882 + +0.6 3,752,882 + +1.2 3,752,904 + +0.6 3,752,904 + +1.5 3,752,984 + -0.1 3,752,984 + +0.1 3,752,984 + -0.5 3,753,074 - +0.4 3,753,102 + -0.3 3,753,102 + -0.1 3,753,364 + Pf1N1B4_3365 0.27 +0.1 3,753,364 + Pf1N1B4_3365 0.27 +0.5 3,753,364 + -0.7 3,753,365 - Pf1N1B4_3365 0.27 -0.4 3,753,365 - Pf1N1B4_3365 0.27 -0.1 3,753,669 + Pf1N1B4_3365 0.51 +1.4 3,753,669 + Pf1N1B4_3365 0.51 -0.6 3,753,778 + Pf1N1B4_3365 0.60 -2.3 3,753,778 + Pf1N1B4_3365 0.60 +1.5 3,753,833 + Pf1N1B4_3365 0.64 +0.6 3,753,834 - Pf1N1B4_3365 0.64 +0.9
Or see this region's nucleotide sequence