Experiment: deuterated glucose in D2O
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt otsA and otsB overlap by 26 nucleotides otsB and araH are separated by 166 nucleotides
b1896: otsA - trehalose-6-phosphate synthase (NCBI), at 1,978,212 to 1,979,636
otsA
b1897: otsB - trehalose-6-phosphate phosphatase, biosynthetic (NCBI), at 1,979,611 to 1,980,411
otsB
b4460: araH - fused L-arabinose transporter subunits of ABC superfamily: membrane components (RefSeq), at 1,980,578 to 1,981,564
araH
Position (kb)
1979
1980
1981 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1978.719 kb on + strand, within otsA at 1978.728 kb on - strand, within otsA at 1978.731 kb on + strand, within otsA at 1978.731 kb on + strand, within otsA at 1978.732 kb on - strand, within otsA at 1978.732 kb on - strand, within otsA at 1978.737 kb on - strand, within otsA at 1978.737 kb on - strand, within otsA at 1978.850 kb on - strand, within otsA at 1978.850 kb on - strand, within otsA at 1978.883 kb on - strand, within otsA at 1978.971 kb on - strand, within otsA at 1978.977 kb on + strand, within otsA at 1978.997 kb on - strand, within otsA at 1978.997 kb on - strand, within otsA at 1979.007 kb on - strand, within otsA at 1979.007 kb on - strand, within otsA at 1979.019 kb on - strand, within otsA at 1979.044 kb on - strand, within otsA at 1979.066 kb on - strand, within otsA at 1979.093 kb on + strand, within otsA at 1979.093 kb on + strand, within otsA at 1979.124 kb on + strand, within otsA at 1979.219 kb on - strand, within otsA at 1979.219 kb on - strand, within otsA at 1979.250 kb on + strand, within otsA at 1979.262 kb on - strand, within otsA at 1979.268 kb on + strand, within otsA at 1979.268 kb on - strand, within otsA at 1979.268 kb on - strand, within otsA at 1979.443 kb on - strand, within otsA at 1979.748 kb on - strand, within otsB at 1979.885 kb on + strand, within otsB at 1979.998 kb on - strand, within otsB at 1980.189 kb on - strand, within otsB at 1980.229 kb on - strand, within otsB at 1980.256 kb on - strand, within otsB at 1980.274 kb on + strand, within otsB at 1980.341 kb on + strand at 1980.827 kb on - strand at 1980.984 kb on + strand, within araH at 1981.002 kb on - strand, within araH at 1981.002 kb on - strand, within araH
Per-strain Table
Position Strand Gene LocusTag Fraction deuterated glucose in D2O remove 1,978,719 + otsA b1896 0.36 -1.2 1,978,728 - otsA b1896 0.36 +1.2 1,978,731 + otsA b1896 0.36 -0.4 1,978,731 + otsA b1896 0.36 -0.3 1,978,732 - otsA b1896 0.36 +1.1 1,978,732 - otsA b1896 0.36 +0.6 1,978,737 - otsA b1896 0.37 +1.0 1,978,737 - otsA b1896 0.37 -0.6 1,978,850 - otsA b1896 0.45 -0.2 1,978,850 - otsA b1896 0.45 +1.6 1,978,883 - otsA b1896 0.47 +1.4 1,978,971 - otsA b1896 0.53 +0.4 1,978,977 + otsA b1896 0.54 +0.5 1,978,997 - otsA b1896 0.55 +0.1 1,978,997 - otsA b1896 0.55 -1.2 1,979,007 - otsA b1896 0.56 +0.6 1,979,007 - otsA b1896 0.56 +3.4 1,979,019 - otsA b1896 0.57 -0.4 1,979,044 - otsA b1896 0.58 +0.3 1,979,066 - otsA b1896 0.60 +2.0 1,979,093 + otsA b1896 0.62 -0.8 1,979,093 + otsA b1896 0.62 +0.9 1,979,124 + otsA b1896 0.64 +0.6 1,979,219 - otsA b1896 0.71 -0.2 1,979,219 - otsA b1896 0.71 -1.0 1,979,250 + otsA b1896 0.73 +1.1 1,979,262 - otsA b1896 0.74 -0.2 1,979,268 + otsA b1896 0.74 -0.3 1,979,268 - otsA b1896 0.74 -1.2 1,979,268 - otsA b1896 0.74 -0.5 1,979,443 - otsA b1896 0.86 +0.4 1,979,748 - otsB b1897 0.17 -2.7 1,979,885 + otsB b1897 0.34 +0.1 1,979,998 - otsB b1897 0.48 -3.4 1,980,189 - otsB b1897 0.72 -1.6 1,980,229 - otsB b1897 0.77 -2.3 1,980,256 - otsB b1897 0.81 -1.2 1,980,274 + otsB b1897 0.83 -0.0 1,980,341 + +0.4 1,980,827 - +0.4 1,980,984 + araH b4460 0.41 -0.2 1,981,002 - araH b4460 0.43 -0.3 1,981,002 - araH b4460 0.43 -0.9
Or see this region's nucleotide sequence