Experiment: 1-ethyl-3-methylimidazolium lysine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2553 and Pf1N1B4_2554 are separated by 184 nucleotides Pf1N1B4_2554 and Pf1N1B4_2555 are separated by 82 nucleotides Pf1N1B4_2555 and Pf1N1B4_2556 are separated by 50 nucleotides
Pf1N1B4_2553 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-), at 2,896,170 to 2,896,817
_2553
Pf1N1B4_2554 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 2,897,002 to 2,897,340
_2554
Pf1N1B4_2555 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,897,423 to 2,898,223
_2555
Pf1N1B4_2556 - FIG00954311: hypothetical protein, at 2,898,274 to 2,899,251
_2556
Position (kb)
2897
2898 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2896.051 kb on + strand at 2896.051 kb on + strand at 2896.051 kb on + strand at 2896.052 kb on - strand at 2896.052 kb on - strand at 2896.065 kb on - strand at 2896.079 kb on + strand at 2896.079 kb on + strand at 2896.079 kb on + strand at 2896.079 kb on + strand at 2896.080 kb on - strand at 2896.102 kb on - strand at 2896.102 kb on - strand at 2896.171 kb on + strand at 2896.171 kb on + strand at 2896.172 kb on - strand at 2896.172 kb on - strand at 2896.851 kb on + strand at 2896.852 kb on - strand at 2896.852 kb on - strand at 2896.955 kb on + strand at 2896.955 kb on + strand at 2897.046 kb on + strand, within Pf1N1B4_2554 at 2897.046 kb on + strand, within Pf1N1B4_2554 at 2897.046 kb on + strand, within Pf1N1B4_2554 at 2897.047 kb on - strand, within Pf1N1B4_2554 at 2897.047 kb on - strand, within Pf1N1B4_2554 at 2897.047 kb on - strand at 2897.057 kb on + strand, within Pf1N1B4_2554 at 2897.058 kb on - strand, within Pf1N1B4_2554 at 2897.058 kb on - strand at 2897.058 kb on - strand, within Pf1N1B4_2554 at 2897.154 kb on + strand, within Pf1N1B4_2554 at 2897.154 kb on + strand, within Pf1N1B4_2554 at 2897.155 kb on - strand, within Pf1N1B4_2554 at 2897.173 kb on - strand, within Pf1N1B4_2554 at 2897.222 kb on - strand, within Pf1N1B4_2554 at 2897.234 kb on - strand, within Pf1N1B4_2554 at 2897.269 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.317 kb on - strand at 2897.317 kb on - strand at 2897.334 kb on + strand at 2897.377 kb on - strand at 2897.387 kb on + strand at 2897.436 kb on + strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.533 kb on + strand, within Pf1N1B4_2555 at 2897.533 kb on + strand at 2897.534 kb on - strand, within Pf1N1B4_2555 at 2897.534 kb on - strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.740 kb on - strand, within Pf1N1B4_2555 at 2897.740 kb on - strand, within Pf1N1B4_2555 at 2897.755 kb on - strand at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2898.292 kb on + strand at 2898.292 kb on + strand at 2898.293 kb on - strand at 2898.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 5 mM remove 2,896,051 + -0.4 2,896,051 + +1.3 2,896,051 + +0.6 2,896,052 - +1.0 2,896,052 - -0.0 2,896,065 - -0.3 2,896,079 + +1.9 2,896,079 + +0.3 2,896,079 + -1.0 2,896,079 + +0.5 2,896,080 - -2.0 2,896,102 - -1.0 2,896,102 - -0.0 2,896,171 + -0.0 2,896,171 + -1.9 2,896,172 - +1.1 2,896,172 - -1.3 2,896,851 + -1.0 2,896,852 - -0.9 2,896,852 - -1.3 2,896,955 + +0.0 2,896,955 + +0.4 2,897,046 + Pf1N1B4_2554 0.13 +0.3 2,897,046 + Pf1N1B4_2554 0.13 +0.9 2,897,046 + Pf1N1B4_2554 0.13 -0.9 2,897,047 - Pf1N1B4_2554 0.13 -2.3 2,897,047 - Pf1N1B4_2554 0.13 -0.3 2,897,047 - +0.7 2,897,057 + Pf1N1B4_2554 0.16 -0.1 2,897,058 - Pf1N1B4_2554 0.17 -0.3 2,897,058 - -0.3 2,897,058 - Pf1N1B4_2554 0.17 +1.3 2,897,154 + Pf1N1B4_2554 0.45 +0.2 2,897,154 + Pf1N1B4_2554 0.45 +1.3 2,897,155 - Pf1N1B4_2554 0.45 +0.1 2,897,173 - Pf1N1B4_2554 0.50 +0.9 2,897,222 - Pf1N1B4_2554 0.65 -1.5 2,897,234 - Pf1N1B4_2554 0.68 +0.8 2,897,269 + Pf1N1B4_2554 0.79 -0.1 2,897,285 + Pf1N1B4_2554 0.83 -1.9 2,897,285 + Pf1N1B4_2554 0.83 +0.0 2,897,285 + Pf1N1B4_2554 0.83 -1.3 2,897,285 + Pf1N1B4_2554 0.83 +1.3 2,897,285 + Pf1N1B4_2554 0.83 -0.7 2,897,285 + Pf1N1B4_2554 0.83 -0.0 2,897,285 + Pf1N1B4_2554 0.83 -0.5 2,897,286 - Pf1N1B4_2554 0.84 -0.4 2,897,286 - Pf1N1B4_2554 0.84 +1.9 2,897,286 - Pf1N1B4_2554 0.84 -1.3 2,897,286 - Pf1N1B4_2554 0.84 +0.6 2,897,317 - +1.3 2,897,317 - +0.3 2,897,334 + -0.1 2,897,377 - -2.2 2,897,387 + -1.0 2,897,436 + -0.8 2,897,437 - -0.9 2,897,437 - -1.7 2,897,437 - -0.0 2,897,437 - -0.5 2,897,533 + Pf1N1B4_2555 0.14 +0.0 2,897,533 + -1.0 2,897,534 - Pf1N1B4_2555 0.14 +1.3 2,897,534 - Pf1N1B4_2555 0.14 +1.3 2,897,568 + Pf1N1B4_2555 0.18 -0.3 2,897,568 + Pf1N1B4_2555 0.18 +0.3 2,897,568 + Pf1N1B4_2555 0.18 +0.2 2,897,568 + Pf1N1B4_2555 0.18 -0.3 2,897,568 + Pf1N1B4_2555 0.18 -0.4 2,897,569 - Pf1N1B4_2555 0.18 -0.9 2,897,569 - Pf1N1B4_2555 0.18 +0.7 2,897,569 - Pf1N1B4_2555 0.18 -0.9 2,897,569 - Pf1N1B4_2555 0.18 -1.5 2,897,569 - Pf1N1B4_2555 0.18 +1.3 2,897,739 + Pf1N1B4_2555 0.39 -0.0 2,897,739 + Pf1N1B4_2555 0.39 -0.0 2,897,739 + Pf1N1B4_2555 0.39 -1.3 2,897,739 + Pf1N1B4_2555 0.39 -0.6 2,897,740 - Pf1N1B4_2555 0.40 +0.6 2,897,740 - Pf1N1B4_2555 0.40 -0.0 2,897,755 - -0.7 2,897,757 + Pf1N1B4_2555 0.42 -0.2 2,897,757 + Pf1N1B4_2555 0.42 +0.9 2,897,757 + Pf1N1B4_2555 0.42 +0.1 2,897,757 + Pf1N1B4_2555 0.42 -0.5 2,897,757 + Pf1N1B4_2555 0.42 -0.9 2,897,757 + Pf1N1B4_2555 0.42 -0.0 2,897,758 - Pf1N1B4_2555 0.42 -0.7 2,897,758 - Pf1N1B4_2555 0.42 +1.8 2,897,758 - Pf1N1B4_2555 0.42 +0.3 2,897,758 - Pf1N1B4_2555 0.42 -2.0 2,897,758 - Pf1N1B4_2555 0.42 +1.3 2,897,758 - Pf1N1B4_2555 0.42 +1.3 2,897,758 - Pf1N1B4_2555 0.42 +0.3 2,897,758 - Pf1N1B4_2555 0.42 -0.8 2,897,758 - Pf1N1B4_2555 0.42 +0.1 2,897,758 - Pf1N1B4_2555 0.42 -0.5 2,897,758 - Pf1N1B4_2555 0.42 -1.2 2,898,292 + -0.7 2,898,292 + -2.8 2,898,293 - +0.3 2,898,293 - -1.4
Or see this region's nucleotide sequence