Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5307 and Pf1N1B4_5308 are separated by 847 nucleotides Pf1N1B4_5308 and Pf1N1B4_5309 are separated by 301 nucleotides Pf1N1B4_5309 and Pf1N1B4_5310 are separated by 48 nucleotides
Pf1N1B4_5307 - hypothetical protein, at 58,801 to 59,055
_5307
Pf1N1B4_5308 - hypothetical protein, at 59,903 to 60,145
_5308
Pf1N1B4_5309 - hypothetical protein, at 60,447 to 60,770
_5309
Pf1N1B4_5310 - hypothetical protein, at 60,819 to 61,040
_5310
Position (kb)
59
60
61 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 58.966 kb on + strand, within Pf1N1B4_5307 at 58.966 kb on + strand, within Pf1N1B4_5307 at 58.967 kb on - strand at 58.967 kb on - strand, within Pf1N1B4_5307 at 59.002 kb on + strand, within Pf1N1B4_5307 at 59.002 kb on + strand, within Pf1N1B4_5307 at 59.003 kb on - strand, within Pf1N1B4_5307 at 59.063 kb on + strand at 59.274 kb on + strand at 59.274 kb on + strand at 59.274 kb on + strand at 59.275 kb on - strand at 59.275 kb on - strand at 59.501 kb on + strand at 59.530 kb on + strand at 59.530 kb on + strand at 59.530 kb on + strand at 59.530 kb on + strand at 59.530 kb on + strand at 59.531 kb on - strand at 59.534 kb on + strand at 59.534 kb on + strand at 59.535 kb on - strand at 59.581 kb on - strand at 59.659 kb on + strand at 59.659 kb on + strand at 59.659 kb on + strand at 59.660 kb on - strand at 59.660 kb on - strand at 59.660 kb on - strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.663 kb on + strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.664 kb on - strand at 59.669 kb on + strand at 59.827 kb on - strand at 59.841 kb on + strand at 59.845 kb on + strand at 59.845 kb on + strand at 59.845 kb on + strand at 59.845 kb on + strand at 59.845 kb on + strand at 59.846 kb on - strand at 59.846 kb on - strand at 59.846 kb on - strand at 60.007 kb on + strand, within Pf1N1B4_5308 at 60.007 kb on + strand, within Pf1N1B4_5308 at 60.007 kb on + strand, within Pf1N1B4_5308 at 60.008 kb on - strand, within Pf1N1B4_5308 at 60.008 kb on - strand, within Pf1N1B4_5308 at 60.008 kb on - strand, within Pf1N1B4_5308 at 60.008 kb on - strand at 60.008 kb on - strand, within Pf1N1B4_5308 at 60.029 kb on + strand at 60.029 kb on + strand at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand, within Pf1N1B4_5308 at 60.030 kb on - strand at 60.243 kb on + strand at 60.274 kb on + strand at 60.275 kb on - strand at 60.349 kb on + strand at 60.350 kb on - strand at 60.350 kb on - strand at 60.447 kb on - strand at 60.460 kb on + strand at 60.559 kb on - strand, within Pf1N1B4_5309 at 60.559 kb on - strand, within Pf1N1B4_5309 at 60.559 kb on - strand, within Pf1N1B4_5309 at 60.559 kb on - strand, within Pf1N1B4_5309 at 60.869 kb on + strand, within Pf1N1B4_5310 at 60.869 kb on + strand, within Pf1N1B4_5310 at 60.869 kb on + strand at 60.869 kb on + strand, within Pf1N1B4_5310 at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.870 kb on - strand at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.870 kb on - strand, within Pf1N1B4_5310 at 60.885 kb on + strand, within Pf1N1B4_5310 at 61.074 kb on + strand at 61.075 kb on - strand at 61.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 58,966 + Pf1N1B4_5307 0.65 -0.2 58,966 + Pf1N1B4_5307 0.65 +0.6 58,967 - -0.4 58,967 - Pf1N1B4_5307 0.65 +0.2 59,002 + Pf1N1B4_5307 0.79 -0.1 59,002 + Pf1N1B4_5307 0.79 +0.2 59,003 - Pf1N1B4_5307 0.79 -1.0 59,063 + -0.2 59,274 + -1.2 59,274 + -1.1 59,274 + -0.0 59,275 - +0.6 59,275 - +0.2 59,501 + -2.2 59,530 + +0.2 59,530 + +2.4 59,530 + +0.8 59,530 + +1.9 59,530 + +0.4 59,531 - +0.2 59,534 + +0.2 59,534 + -1.1 59,535 - +1.2 59,581 - -0.1 59,659 + -0.1 59,659 + +0.2 59,659 + +1.5 59,660 - +2.2 59,660 - -0.4 59,660 - +1.0 59,663 + -0.0 59,663 + -0.4 59,663 + +1.4 59,663 + -0.1 59,663 + -0.4 59,663 + -0.0 59,663 + -1.5 59,663 + +1.0 59,663 + -0.1 59,663 + +3.2 59,663 + -0.4 59,663 + +0.6 59,663 + +0.0 59,663 + -1.8 59,663 + +0.5 59,663 + -1.4 59,663 + -1.4 59,663 + +0.2 59,664 - +1.9 59,664 - -0.8 59,664 - -0.4 59,664 - -0.7 59,664 - +0.1 59,664 - -0.7 59,664 - -0.1 59,664 - -0.8 59,669 + -1.0 59,827 - -0.5 59,841 + +0.4 59,845 + +0.2 59,845 + +1.6 59,845 + +0.2 59,845 + +2.8 59,845 + -0.5 59,846 - -1.0 59,846 - -0.4 59,846 - +0.2 60,007 + Pf1N1B4_5308 0.43 -2.6 60,007 + Pf1N1B4_5308 0.43 +1.2 60,007 + Pf1N1B4_5308 0.43 -2.0 60,008 - Pf1N1B4_5308 0.43 -1.1 60,008 - Pf1N1B4_5308 0.43 -0.8 60,008 - Pf1N1B4_5308 0.43 +1.2 60,008 - +0.5 60,008 - Pf1N1B4_5308 0.43 -0.8 60,029 + -1.1 60,029 + -0.4 60,030 - Pf1N1B4_5308 0.52 -0.8 60,030 - Pf1N1B4_5308 0.52 -1.1 60,030 - Pf1N1B4_5308 0.52 -0.2 60,030 - Pf1N1B4_5308 0.52 -0.8 60,030 - Pf1N1B4_5308 0.52 -0.4 60,030 - Pf1N1B4_5308 0.52 -0.4 60,030 - Pf1N1B4_5308 0.52 -0.4 60,030 - Pf1N1B4_5308 0.52 -0.4 60,030 - Pf1N1B4_5308 0.52 -0.7 60,030 - +1.2 60,243 + -0.2 60,274 + +1.6 60,275 - +1.2 60,349 + -1.0 60,350 - +0.8 60,350 - -0.8 60,447 - -2.2 60,460 + -0.5 60,559 - Pf1N1B4_5309 0.35 +0.2 60,559 - Pf1N1B4_5309 0.35 -0.8 60,559 - Pf1N1B4_5309 0.35 +0.5 60,559 - Pf1N1B4_5309 0.35 -0.5 60,869 + Pf1N1B4_5310 0.23 +2.9 60,869 + Pf1N1B4_5310 0.23 +0.5 60,869 + +1.2 60,869 + Pf1N1B4_5310 0.23 +0.6 60,870 - Pf1N1B4_5310 0.23 +0.4 60,870 - Pf1N1B4_5310 0.23 -1.0 60,870 - Pf1N1B4_5310 0.23 -0.3 60,870 - Pf1N1B4_5310 0.23 +0.2 60,870 - +2.8 60,870 - Pf1N1B4_5310 0.23 -0.9 60,870 - Pf1N1B4_5310 0.23 -1.8 60,870 - Pf1N1B4_5310 0.23 +1.3 60,885 + Pf1N1B4_5310 0.30 +1.2 61,074 + -0.1 61,075 - +1.5 61,098 - -1.1
Or see this region's nucleotide sequence