Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5975 and Pf1N1B4_5976 are separated by 115 nucleotides Pf1N1B4_5976 and Pf1N1B4_5977 are separated by 71 nucleotides
Pf1N1B4_5975 - 2-methylaconitate racemase, at 422,673 to 423,701
_5975
Pf1N1B4_5976 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 423,817 to 424,734
_5976
Pf1N1B4_5977 - Transporter, MFS superfamily, at 424,806 to 426,203
_5977
Position (kb) 423
424
425 Strain fitness (log2 ratio) -2
-1
0
1
2 at 423.335 kb on + strand at 423.335 kb on + strand, within Pf1N1B4_5975 at 423.336 kb on - strand, within Pf1N1B4_5975 at 423.373 kb on + strand, within Pf1N1B4_5975 at 423.374 kb on - strand, within Pf1N1B4_5975 at 423.589 kb on - strand, within Pf1N1B4_5975 at 423.589 kb on - strand, within Pf1N1B4_5975 at 423.739 kb on - strand at 423.739 kb on - strand at 423.831 kb on + strand at 423.832 kb on - strand at 424.192 kb on + strand, within Pf1N1B4_5976 at 424.228 kb on + strand at 424.228 kb on + strand, within Pf1N1B4_5976 at 424.246 kb on + strand, within Pf1N1B4_5976 at 424.528 kb on + strand, within Pf1N1B4_5976 at 424.529 kb on - strand, within Pf1N1B4_5976 at 424.820 kb on - strand at 424.862 kb on - strand at 425.598 kb on + strand, within Pf1N1B4_5977 at 425.599 kb on - strand at 425.599 kb on - strand, within Pf1N1B4_5977 at 425.625 kb on + strand, within Pf1N1B4_5977 at 425.721 kb on + strand, within Pf1N1B4_5977
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 423,335 + -0.9 423,335 + Pf1N1B4_5975 0.64 -0.1 423,336 - Pf1N1B4_5975 0.64 -1.9 423,373 + Pf1N1B4_5975 0.68 +0.4 423,374 - Pf1N1B4_5975 0.68 +1.4 423,589 - Pf1N1B4_5975 0.89 -0.5 423,589 - Pf1N1B4_5975 0.89 +0.9 423,739 - +0.4 423,739 - +1.0 423,831 + -0.4 423,832 - -0.9 424,192 + Pf1N1B4_5976 0.41 +1.8 424,228 + -1.7 424,228 + Pf1N1B4_5976 0.45 +0.3 424,246 + Pf1N1B4_5976 0.47 -2.3 424,528 + Pf1N1B4_5976 0.77 +0.0 424,529 - Pf1N1B4_5976 0.78 +0.7 424,820 - -0.3 424,862 - -0.3 425,598 + Pf1N1B4_5977 0.57 -0.9 425,599 - +0.1 425,599 - Pf1N1B4_5977 0.57 +0.1 425,625 + Pf1N1B4_5977 0.59 +0.7 425,721 + Pf1N1B4_5977 0.65 -0.0
Or see this region's nucleotide sequence