Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5650 and Pf1N1B4_5651 are separated by 184 nucleotides Pf1N1B4_5651 and Pf1N1B4_5652 are separated by 119 nucleotides Pf1N1B4_5652 and Pf1N1B4_5653 are separated by 149 nucleotides
Pf1N1B4_5650 - Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes, at 77,942 to 79,588
_5650
Pf1N1B4_5651 - ChlD component of cobalt chelatase involved in B12 biosynthesis, at 79,773 to 80,318
_5651
Pf1N1B4_5652 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 80,438 to 81,448
_5652
Pf1N1B4_5653 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 81,598 to 85,464
_5653
Position (kb) 80
81
82 Strain fitness (log2 ratio) -1
0
1 at 79.539 kb on + strand at 79.540 kb on - strand at 79.797 kb on - strand at 80.247 kb on + strand, within Pf1N1B4_5651 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.141 kb on + strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.142 kb on - strand, within Pf1N1B4_5652 at 81.171 kb on - strand, within Pf1N1B4_5652 at 81.614 kb on - strand at 81.614 kb on - strand at 81.768 kb on - strand at 81.768 kb on - strand at 81.870 kb on + strand at 81.870 kb on + strand at 81.871 kb on - strand at 81.871 kb on - strand at 82.058 kb on + strand, within Pf1N1B4_5653
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 79,539 + +0.0 79,540 - +1.6 79,797 - +0.6 80,247 + Pf1N1B4_5651 0.87 +0.6 81,141 + Pf1N1B4_5652 0.70 +1.2 81,141 + Pf1N1B4_5652 0.70 -0.2 81,142 - Pf1N1B4_5652 0.70 +1.0 81,142 - Pf1N1B4_5652 0.70 +0.2 81,142 - Pf1N1B4_5652 0.70 +0.5 81,142 - Pf1N1B4_5652 0.70 +1.2 81,142 - Pf1N1B4_5652 0.70 +0.5 81,171 - Pf1N1B4_5652 0.73 +0.3 81,614 - -0.7 81,614 - +0.1 81,768 - -0.0 81,768 - +1.5 81,870 + +1.3 81,870 + +0.6 81,871 - -0.1 81,871 - -0.2 82,058 + Pf1N1B4_5653 0.12 -0.1
Or see this region's nucleotide sequence