Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4563

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4562 and Pf1N1B4_4563 are separated by 206 nucleotidesPf1N1B4_4563 and Pf1N1B4_4564 are separated by 52 nucleotides Pf1N1B4_4562 - Serine transporter, at 5,032,762 to 5,034,042 _4562 Pf1N1B4_4563 - Sulfate transporter, at 5,034,249 to 5,035,925 _4563 Pf1N1B4_4564 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 5,035,978 to 5,038,578 _4564 Position (kb) 5034 5035 5036Strain fitness (log2 ratio) -2 -1 0 1 2at 5033.700 kb on + strand, within Pf1N1B4_4562at 5033.700 kb on + strand, within Pf1N1B4_4562at 5033.725 kb on + strand, within Pf1N1B4_4562at 5034.025 kb on + strandat 5034.026 kb on - strandat 5034.048 kb on - strandat 5034.139 kb on + strandat 5034.293 kb on - strandat 5034.313 kb on + strandat 5034.314 kb on - strandat 5034.314 kb on - strandat 5034.857 kb on - strandat 5034.988 kb on + strand, within Pf1N1B4_4563at 5035.029 kb on + strand, within Pf1N1B4_4563at 5035.269 kb on + strand, within Pf1N1B4_4563at 5035.269 kb on + strand, within Pf1N1B4_4563at 5035.356 kb on + strand, within Pf1N1B4_4563at 5035.530 kb on + strand, within Pf1N1B4_4563at 5035.530 kb on + strand, within Pf1N1B4_4563at 5035.923 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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5,033,700 + Pf1N1B4_4562 0.73 -1.4
5,033,700 + Pf1N1B4_4562 0.73 -2.5
5,033,725 + Pf1N1B4_4562 0.75 -1.5
5,034,025 + +0.5
5,034,026 - +1.5
5,034,048 - -0.1
5,034,139 + -1.4
5,034,293 - +0.0
5,034,313 + +1.0
5,034,314 - +0.3
5,034,314 - -0.4
5,034,857 - -0.1
5,034,988 + Pf1N1B4_4563 0.44 -0.5
5,035,029 + Pf1N1B4_4563 0.47 +1.8
5,035,269 + Pf1N1B4_4563 0.61 +0.5
5,035,269 + Pf1N1B4_4563 0.61 +0.1
5,035,356 + Pf1N1B4_4563 0.66 -0.7
5,035,530 + Pf1N1B4_4563 0.76 +1.5
5,035,530 + Pf1N1B4_4563 0.76 +1.5
5,035,923 + -0.5

Or see this region's nucleotide sequence