Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_41

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_40 and Pf1N1B4_41 are separated by 367 nucleotidesPf1N1B4_41 and Pf1N1B4_42 overlap by 4 nucleotides Pf1N1B4_40 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 46,683 to 48,254 _40 Pf1N1B4_41 - hypothetical protein, at 48,622 to 49,104 _41 Pf1N1B4_42 - hypothetical protein, at 49,101 to 50,606 _42 Position (kb) 48 49 50Strain fitness (log2 ratio) -2 -1 0 1 2 3at 47.659 kb on + strand, within Pf1N1B4_40at 47.660 kb on - strand, within Pf1N1B4_40at 47.660 kb on - strand, within Pf1N1B4_40at 47.902 kb on - strand, within Pf1N1B4_40at 48.134 kb on - strandat 48.249 kb on - strandat 48.273 kb on + strandat 48.302 kb on - strandat 48.316 kb on + strandat 48.316 kb on + strandat 48.338 kb on + strandat 48.338 kb on + strandat 48.353 kb on + strandat 48.382 kb on + strandat 48.382 kb on + strandat 48.424 kb on + strandat 48.424 kb on + strandat 48.425 kb on - strandat 48.425 kb on - strandat 48.429 kb on - strandat 48.458 kb on - strandat 48.507 kb on + strandat 48.507 kb on + strandat 48.589 kb on + strandat 48.589 kb on + strandat 48.590 kb on - strandat 48.627 kb on + strandat 48.627 kb on + strandat 48.627 kb on + strandat 48.628 kb on - strandat 48.727 kb on + strand, within Pf1N1B4_41at 48.727 kb on + strand, within Pf1N1B4_41at 48.727 kb on + strand, within Pf1N1B4_41at 48.728 kb on - strand, within Pf1N1B4_41at 48.736 kb on + strand, within Pf1N1B4_41at 48.737 kb on - strand, within Pf1N1B4_41at 48.737 kb on - strand, within Pf1N1B4_41at 49.213 kb on + strandat 49.214 kb on - strandat 49.214 kb on - strandat 49.412 kb on + strand, within Pf1N1B4_42at 49.477 kb on + strandat 49.478 kb on - strand, within Pf1N1B4_42at 49.553 kb on + strand, within Pf1N1B4_42at 49.726 kb on + strand, within Pf1N1B4_42at 49.726 kb on + strand, within Pf1N1B4_42at 49.726 kb on + strand, within Pf1N1B4_42at 49.726 kb on + strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.727 kb on - strand, within Pf1N1B4_42at 49.814 kb on - strand, within Pf1N1B4_42at 49.829 kb on - strand, within Pf1N1B4_42at 49.870 kb on + strand, within Pf1N1B4_42at 49.870 kb on + strand, within Pf1N1B4_42at 49.984 kb on + strand, within Pf1N1B4_42at 49.984 kb on + strand, within Pf1N1B4_42at 49.985 kb on - strand, within Pf1N1B4_42at 49.999 kb on + strand, within Pf1N1B4_42at 49.999 kb on + strand, within Pf1N1B4_42at 50.000 kb on - strand, within Pf1N1B4_42at 50.000 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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47,659 + Pf1N1B4_40 0.62 +1.4
47,660 - Pf1N1B4_40 0.62 -0.9
47,660 - Pf1N1B4_40 0.62 -1.8
47,902 - Pf1N1B4_40 0.78 +0.0
48,134 - -0.2
48,249 - +0.5
48,273 + -0.2
48,302 - -0.5
48,316 + -0.8
48,316 + +0.8
48,338 + -0.1
48,338 + +1.1
48,353 + +0.2
48,382 + +0.8
48,382 + +0.5
48,424 + -0.8
48,424 + -1.2
48,425 - +1.0
48,425 - +0.8
48,429 - +1.6
48,458 - +0.1
48,507 + +2.8
48,507 + -1.7
48,589 + -0.2
48,589 + -1.8
48,590 - +2.4
48,627 + -0.2
48,627 + -0.2
48,627 + -0.4
48,628 - -0.8
48,727 + Pf1N1B4_41 0.22 +2.2
48,727 + Pf1N1B4_41 0.22 +0.5
48,727 + Pf1N1B4_41 0.22 +0.8
48,728 - Pf1N1B4_41 0.22 -1.4
48,736 + Pf1N1B4_41 0.24 +0.2
48,737 - Pf1N1B4_41 0.24 -2.0
48,737 - Pf1N1B4_41 0.24 -0.2
49,213 + -1.3
49,214 - +1.4
49,214 - +1.8
49,412 + Pf1N1B4_42 0.21 +0.0
49,477 + +2.1
49,478 - Pf1N1B4_42 0.25 +2.1
49,553 + Pf1N1B4_42 0.30 +1.2
49,726 + Pf1N1B4_42 0.42 +1.6
49,726 + Pf1N1B4_42 0.42 -0.6
49,726 + Pf1N1B4_42 0.42 +1.4
49,726 + Pf1N1B4_42 0.42 +1.8
49,727 - Pf1N1B4_42 0.42 -0.5
49,727 - Pf1N1B4_42 0.42 -0.6
49,727 - Pf1N1B4_42 0.42 -0.2
49,727 - Pf1N1B4_42 0.42 +1.0
49,727 - Pf1N1B4_42 0.42 -1.2
49,727 - Pf1N1B4_42 0.42 -2.7
49,727 - Pf1N1B4_42 0.42 +1.4
49,727 - Pf1N1B4_42 0.42 -0.2
49,727 - Pf1N1B4_42 0.42 -1.7
49,727 - Pf1N1B4_42 0.42 +0.5
49,727 - Pf1N1B4_42 0.42 +0.8
49,814 - Pf1N1B4_42 0.47 +0.1
49,829 - Pf1N1B4_42 0.48 +0.4
49,870 + Pf1N1B4_42 0.51 +0.2
49,870 + Pf1N1B4_42 0.51 +0.1
49,984 + Pf1N1B4_42 0.59 +2.1
49,984 + Pf1N1B4_42 0.59 +0.0
49,985 - Pf1N1B4_42 0.59 +1.2
49,999 + Pf1N1B4_42 0.60 -0.2
49,999 + Pf1N1B4_42 0.60 -0.2
50,000 - Pf1N1B4_42 0.60 +0.8
50,000 - +0.8

Or see this region's nucleotide sequence