Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4062 and Pf1N1B4_4063 overlap by 1 nucleotides Pf1N1B4_4063 and Pf1N1B4_4064 are separated by 15 nucleotides
Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619
_4062
Pf1N1B4_4063 - CrcB protein, at 4,517,619 to 4,517,993
_4063
Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289
_4064
Position (kb)
4517
4518 Strain fitness (log2 ratio)
-1
0
1 at 4516.768 kb on + strand, within Pf1N1B4_4062 at 4516.769 kb on - strand, within Pf1N1B4_4062 at 4517.041 kb on + strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.065 kb on - strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.144 kb on - strand, within Pf1N1B4_4062 at 4517.287 kb on - strand, within Pf1N1B4_4062 at 4517.290 kb on + strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.323 kb on + strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.488 kb on + strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.594 kb on + strand at 4517.595 kb on - strand at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.941 kb on + strand, within Pf1N1B4_4063
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,516,768 + Pf1N1B4_4062 0.36 +0.7 4,516,769 - Pf1N1B4_4062 0.36 +0.8 4,517,041 + Pf1N1B4_4062 0.56 +0.1 4,517,042 - Pf1N1B4_4062 0.56 -1.7 4,517,042 - Pf1N1B4_4062 0.56 -0.2 4,517,065 - Pf1N1B4_4062 0.58 -0.5 4,517,143 + Pf1N1B4_4062 0.64 -0.3 4,517,143 + Pf1N1B4_4062 0.64 -0.3 4,517,144 - Pf1N1B4_4062 0.64 +0.2 4,517,287 - Pf1N1B4_4062 0.75 -1.1 4,517,290 + Pf1N1B4_4062 0.75 +0.1 4,517,291 - Pf1N1B4_4062 0.75 -0.3 4,517,291 - Pf1N1B4_4062 0.75 -1.3 4,517,323 + Pf1N1B4_4062 0.78 +0.7 4,517,324 - Pf1N1B4_4062 0.78 -0.6 4,517,324 - Pf1N1B4_4062 0.78 -0.3 4,517,488 + -0.7 4,517,489 - -1.1 4,517,489 - +1.7 4,517,489 - -0.5 4,517,594 + -0.2 4,517,595 - -1.7 4,517,721 + Pf1N1B4_4063 0.27 +0.1 4,517,721 + Pf1N1B4_4063 0.27 +0.4 4,517,773 + Pf1N1B4_4063 0.41 +1.1 4,517,773 + Pf1N1B4_4063 0.41 -0.0 4,517,773 + Pf1N1B4_4063 0.41 +0.9 4,517,773 + Pf1N1B4_4063 0.41 +1.1 4,517,773 + Pf1N1B4_4063 0.41 +0.1 4,517,773 + Pf1N1B4_4063 0.41 +0.3 4,517,941 + Pf1N1B4_4063 0.86 +0.1
Or see this region's nucleotide sequence