Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3612 and Pf1N1B4_3613 are separated by 47 nucleotides Pf1N1B4_3613 and Pf1N1B4_3614 are separated by 60 nucleotides Pf1N1B4_3614 and Pf1N1B4_3615 are separated by 9 nucleotides
Pf1N1B4_3612 - Flagellar hook-length control protein FliK, at 4,022,604 to 4,023,965
_3612
Pf1N1B4_3613 - hypothetical protein, at 4,024,013 to 4,024,129
_3613
Pf1N1B4_3614 - Flagellar biosynthesis protein FliL, at 4,024,190 to 4,024,693
_3614
Pf1N1B4_3615 - Flagellar motor switch protein FliM, at 4,024,703 to 4,025,671
_3615
Position (kb)
4024
4025 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.564 kb on + strand at 4023.564 kb on + strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.565 kb on - strand, within Pf1N1B4_3612 at 4023.954 kb on + strand at 4023.954 kb on + strand at 4023.958 kb on - strand at 4023.958 kb on - strand at 4023.958 kb on - strand at 4024.015 kb on - strand at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.029 kb on - strand, within Pf1N1B4_3613 at 4024.129 kb on + strand at 4024.184 kb on - strand at 4024.184 kb on - strand at 4024.195 kb on + strand at 4024.196 kb on - strand at 4024.418 kb on + strand, within Pf1N1B4_3614 at 4024.418 kb on + strand, within Pf1N1B4_3614 at 4024.418 kb on + strand, within Pf1N1B4_3614 at 4024.419 kb on - strand, within Pf1N1B4_3614 at 4024.419 kb on - strand, within Pf1N1B4_3614 at 4024.419 kb on - strand, within Pf1N1B4_3614 at 4024.442 kb on + strand, within Pf1N1B4_3614 at 4024.443 kb on - strand, within Pf1N1B4_3614 at 4024.443 kb on - strand, within Pf1N1B4_3614 at 4024.443 kb on - strand, within Pf1N1B4_3614 at 4024.691 kb on + strand at 4024.692 kb on - strand at 4024.814 kb on + strand, within Pf1N1B4_3615 at 4024.815 kb on - strand, within Pf1N1B4_3615 at 4024.815 kb on - strand, within Pf1N1B4_3615 at 4024.815 kb on - strand, within Pf1N1B4_3615 at 4024.977 kb on - strand, within Pf1N1B4_3615 at 4024.977 kb on - strand, within Pf1N1B4_3615 at 4024.991 kb on + strand, within Pf1N1B4_3615 at 4024.992 kb on - strand, within Pf1N1B4_3615
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,023,564 + Pf1N1B4_3612 0.70 -0.5 4,023,564 + Pf1N1B4_3612 0.70 -0.7 4,023,564 + Pf1N1B4_3612 0.70 +0.4 4,023,564 + Pf1N1B4_3612 0.70 -0.5 4,023,564 + Pf1N1B4_3612 0.70 -2.0 4,023,564 + Pf1N1B4_3612 0.70 +0.8 4,023,564 + +3.2 4,023,564 + Pf1N1B4_3612 0.70 +0.8 4,023,565 - Pf1N1B4_3612 0.71 +1.6 4,023,565 - Pf1N1B4_3612 0.71 +1.4 4,023,565 - Pf1N1B4_3612 0.71 +2.1 4,023,565 - Pf1N1B4_3612 0.71 -1.2 4,023,565 - Pf1N1B4_3612 0.71 -0.7 4,023,954 + +0.8 4,023,954 + -0.7 4,023,958 - +0.3 4,023,958 - -0.5 4,023,958 - +0.8 4,024,015 - +0.1 4,024,029 - Pf1N1B4_3613 0.14 +1.8 4,024,029 - Pf1N1B4_3613 0.14 +0.6 4,024,029 - +0.3 4,024,029 - Pf1N1B4_3613 0.14 -0.2 4,024,029 - Pf1N1B4_3613 0.14 +1.8 4,024,029 - Pf1N1B4_3613 0.14 -1.0 4,024,129 + +0.3 4,024,184 - +0.3 4,024,184 - -2.5 4,024,195 + +1.4 4,024,196 - +0.8 4,024,418 + Pf1N1B4_3614 0.45 +0.4 4,024,418 + Pf1N1B4_3614 0.45 -2.3 4,024,418 + Pf1N1B4_3614 0.45 +0.6 4,024,419 - Pf1N1B4_3614 0.45 -2.0 4,024,419 - Pf1N1B4_3614 0.45 -0.0 4,024,419 - Pf1N1B4_3614 0.45 +0.5 4,024,442 + Pf1N1B4_3614 0.50 -0.3 4,024,443 - Pf1N1B4_3614 0.50 -0.7 4,024,443 - Pf1N1B4_3614 0.50 +0.2 4,024,443 - Pf1N1B4_3614 0.50 +0.6 4,024,691 + +1.8 4,024,692 - -1.3 4,024,814 + Pf1N1B4_3615 0.11 -2.2 4,024,815 - Pf1N1B4_3615 0.12 -1.0 4,024,815 - Pf1N1B4_3615 0.12 +2.0 4,024,815 - Pf1N1B4_3615 0.12 +1.6 4,024,977 - Pf1N1B4_3615 0.28 +0.8 4,024,977 - Pf1N1B4_3615 0.28 +1.2 4,024,991 + Pf1N1B4_3615 0.30 -0.7 4,024,992 - Pf1N1B4_3615 0.30 -0.9
Or see this region's nucleotide sequence