Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2328 and Pf1N1B4_2329 are separated by 74 nucleotides Pf1N1B4_2329 and Pf1N1B4_2330 are separated by 72 nucleotides Pf1N1B4_2330 and Pf1N1B4_2331 are separated by 60 nucleotides
Pf1N1B4_2328 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 2,648,347 to 2,649,069
_2328
Pf1N1B4_2329 - COG2363, at 2,649,144 to 2,649,515
_2329
Pf1N1B4_2330 - Sulfur carrier protein ThiS, at 2,649,588 to 2,649,806
_2330
Pf1N1B4_2331 - Thiazole biosynthesis protein ThiG, at 2,649,867 to 2,650,661
_2331
Position (kb)
2649
2650 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2648.286 kb on - strand at 2648.324 kb on - strand at 2648.324 kb on - strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.393 kb on + strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.394 kb on - strand at 2648.397 kb on + strand at 2648.397 kb on + strand at 2648.398 kb on - strand at 2648.407 kb on + strand at 2648.407 kb on + strand at 2648.407 kb on + strand at 2648.408 kb on - strand at 2648.408 kb on - strand at 2648.408 kb on - strand at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.453 kb on + strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.454 kb on - strand, within Pf1N1B4_2328 at 2648.510 kb on - strand, within Pf1N1B4_2328 at 2648.615 kb on + strand, within Pf1N1B4_2328 at 2648.615 kb on + strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2648.616 kb on - strand, within Pf1N1B4_2328 at 2649.007 kb on + strand at 2649.007 kb on + strand at 2649.058 kb on + strand at 2649.069 kb on + strand at 2649.069 kb on + strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.105 kb on + strand at 2649.105 kb on + strand at 2649.106 kb on - strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.276 kb on + strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.290 kb on + strand, within Pf1N1B4_2329 at 2649.310 kb on + strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.784 kb on + strand, within Pf1N1B4_2330 at 2649.852 kb on + strand at 2649.881 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.912 kb on - strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.916 kb on - strand at 2649.934 kb on + strand at 2649.934 kb on + strand at 2649.935 kb on - strand at 2649.939 kb on - strand at 2650.083 kb on + strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.098 kb on - strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,648,286 - +0.6 2,648,324 - -1.0 2,648,324 - -0.1 2,648,393 + +0.1 2,648,393 + +2.5 2,648,393 + +1.3 2,648,393 + +0.9 2,648,393 + +0.6 2,648,393 + +1.9 2,648,394 - -0.7 2,648,394 - +1.0 2,648,394 - +2.6 2,648,394 - -0.9 2,648,394 - -0.2 2,648,394 - -0.1 2,648,394 - +2.3 2,648,394 - -0.6 2,648,397 + +0.4 2,648,397 + +0.5 2,648,398 - -0.3 2,648,407 + -0.2 2,648,407 + +1.3 2,648,407 + +0.1 2,648,408 - +0.3 2,648,408 - -0.7 2,648,408 - -1.0 2,648,453 + Pf1N1B4_2328 0.15 -1.0 2,648,453 + Pf1N1B4_2328 0.15 -0.7 2,648,453 + Pf1N1B4_2328 0.15 -1.9 2,648,453 + Pf1N1B4_2328 0.15 -0.4 2,648,453 + Pf1N1B4_2328 0.15 -0.1 2,648,453 + Pf1N1B4_2328 0.15 -0.2 2,648,453 + Pf1N1B4_2328 0.15 -1.0 2,648,454 - Pf1N1B4_2328 0.15 -0.0 2,648,454 - Pf1N1B4_2328 0.15 +0.7 2,648,454 - Pf1N1B4_2328 0.15 -1.7 2,648,454 - Pf1N1B4_2328 0.15 +0.9 2,648,454 - Pf1N1B4_2328 0.15 -1.2 2,648,454 - Pf1N1B4_2328 0.15 +0.2 2,648,454 - Pf1N1B4_2328 0.15 -0.8 2,648,454 - Pf1N1B4_2328 0.15 -1.3 2,648,454 - Pf1N1B4_2328 0.15 +1.6 2,648,454 - Pf1N1B4_2328 0.15 -0.4 2,648,510 - Pf1N1B4_2328 0.23 -1.0 2,648,615 + Pf1N1B4_2328 0.37 -1.7 2,648,615 + Pf1N1B4_2328 0.37 +0.3 2,648,616 - Pf1N1B4_2328 0.37 +3.3 2,648,616 - Pf1N1B4_2328 0.37 +0.3 2,648,616 - Pf1N1B4_2328 0.37 +0.2 2,648,616 - Pf1N1B4_2328 0.37 -1.5 2,648,616 - Pf1N1B4_2328 0.37 +2.3 2,648,616 - Pf1N1B4_2328 0.37 +0.1 2,649,007 + +0.0 2,649,007 + -1.5 2,649,058 + -0.9 2,649,069 + -1.3 2,649,069 + -1.7 2,649,070 - -2.2 2,649,070 - -1.7 2,649,070 - +2.3 2,649,105 + +0.2 2,649,105 + -0.0 2,649,106 - +0.5 2,649,107 + -0.7 2,649,107 + +1.5 2,649,107 + -0.8 2,649,107 + +0.6 2,649,107 + +0.6 2,649,107 + -0.5 2,649,107 + +0.3 2,649,107 + -0.7 2,649,107 + -1.0 2,649,107 + +1.0 2,649,107 + +0.7 2,649,107 + +1.0 2,649,108 - -1.2 2,649,108 - +0.1 2,649,108 - +0.3 2,649,108 - +1.3 2,649,108 - -0.7 2,649,108 - -0.1 2,649,108 - -0.8 2,649,108 - +1.3 2,649,108 - -0.7 2,649,108 - -0.8 2,649,108 - -1.3 2,649,108 - -2.7 2,649,108 - -1.3 2,649,108 - +1.7 2,649,108 - +0.7 2,649,108 - +0.7 2,649,276 + Pf1N1B4_2329 0.35 +1.0 2,649,277 - Pf1N1B4_2329 0.36 -1.3 2,649,277 - Pf1N1B4_2329 0.36 +0.9 2,649,277 - Pf1N1B4_2329 0.36 +0.8 2,649,277 - Pf1N1B4_2329 0.36 +1.8 2,649,277 - Pf1N1B4_2329 0.36 +0.9 2,649,277 - Pf1N1B4_2329 0.36 +0.6 2,649,277 - Pf1N1B4_2329 0.36 +1.7 2,649,290 + Pf1N1B4_2329 0.39 +1.5 2,649,310 + Pf1N1B4_2329 0.45 -1.0 2,649,311 - Pf1N1B4_2329 0.45 +0.6 2,649,311 - Pf1N1B4_2329 0.45 -0.4 2,649,402 + Pf1N1B4_2329 0.69 +0.2 2,649,402 + Pf1N1B4_2329 0.69 +0.9 2,649,402 + Pf1N1B4_2329 0.69 -1.3 2,649,784 + Pf1N1B4_2330 0.89 -0.6 2,649,852 + -0.2 2,649,881 + +0.3 2,649,911 + -1.5 2,649,911 + -1.1 2,649,911 + -2.3 2,649,912 - -0.0 2,649,915 + -0.5 2,649,915 + -0.7 2,649,915 + -1.0 2,649,915 + -1.0 2,649,916 - -0.3 2,649,934 + +0.1 2,649,934 + +0.3 2,649,935 - +1.3 2,649,939 - +1.3 2,650,083 + Pf1N1B4_2331 0.27 +1.3 2,650,084 - Pf1N1B4_2331 0.27 -1.0 2,650,084 - Pf1N1B4_2331 0.27 +1.9 2,650,098 - Pf1N1B4_2331 0.29 +0.1 2,650,284 + Pf1N1B4_2331 0.52 -1.2 2,650,284 + Pf1N1B4_2331 0.52 +1.0 2,650,284 + Pf1N1B4_2331 0.52 +0.4 2,650,284 + Pf1N1B4_2331 0.52 -0.8 2,650,284 + Pf1N1B4_2331 0.52 -0.7 2,650,285 - Pf1N1B4_2331 0.53 -1.7 2,650,285 - Pf1N1B4_2331 0.53 +2.6
Or see this region's nucleotide sequence