Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_163 and Pf1N1B4_164 overlap by 4 nucleotides Pf1N1B4_164 and Pf1N1B4_165 are separated by 124 nucleotides Pf1N1B4_165 and Pf1N1B4_166 are separated by 139 nucleotides
Pf1N1B4_163 - ADP-ribosylglycohydrolase, at 204,369 to 205,313
_163
Pf1N1B4_164 - ADP-ribosylglycohydrolase family protein, at 205,310 to 205,708
_164
Pf1N1B4_165 - FIG00638667: hypothetical protein, at 205,833 to 206,210
_165
Pf1N1B4_166 - Quinohemoprotein amine dehydrogenase beta subunit (EC 1.4.99.-), at 206,350 to 207,468
_166
Position (kb)
205
206
207 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 205.176 kb on + strand, within Pf1N1B4_163 at 205.315 kb on + strand at 205.353 kb on - strand, within Pf1N1B4_164 at 205.497 kb on - strand, within Pf1N1B4_164 at 205.497 kb on - strand, within Pf1N1B4_164 at 205.581 kb on + strand, within Pf1N1B4_164 at 205.582 kb on - strand, within Pf1N1B4_164 at 205.582 kb on - strand, within Pf1N1B4_164 at 205.670 kb on + strand at 205.670 kb on + strand at 205.706 kb on + strand at 205.706 kb on + strand at 205.706 kb on + strand at 205.706 kb on + strand at 205.707 kb on - strand at 205.707 kb on - strand at 206.094 kb on + strand, within Pf1N1B4_165 at 206.107 kb on - strand, within Pf1N1B4_165 at 206.160 kb on + strand at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand, within Pf1N1B4_165 at 206.160 kb on + strand at 206.160 kb on + strand, within Pf1N1B4_165 at 206.161 kb on - strand at 206.295 kb on + strand at 206.296 kb on - strand at 206.471 kb on + strand, within Pf1N1B4_166 at 206.507 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand at 206.510 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand, within Pf1N1B4_166 at 206.510 kb on + strand at 206.511 kb on - strand, within Pf1N1B4_166 at 206.511 kb on - strand, within Pf1N1B4_166 at 206.561 kb on + strand, within Pf1N1B4_166 at 206.562 kb on - strand, within Pf1N1B4_166 at 206.630 kb on + strand, within Pf1N1B4_166 at 206.630 kb on + strand, within Pf1N1B4_166 at 206.631 kb on - strand, within Pf1N1B4_166 at 206.759 kb on + strand, within Pf1N1B4_166 at 206.783 kb on + strand, within Pf1N1B4_166 at 206.784 kb on - strand at 206.804 kb on + strand, within Pf1N1B4_166 at 206.804 kb on + strand, within Pf1N1B4_166 at 206.804 kb on + strand at 206.805 kb on + strand, within Pf1N1B4_166 at 206.805 kb on - strand, within Pf1N1B4_166 at 206.805 kb on - strand, within Pf1N1B4_166 at 206.805 kb on - strand, within Pf1N1B4_166 at 206.826 kb on - strand, within Pf1N1B4_166 at 206.826 kb on - strand, within Pf1N1B4_166 at 206.895 kb on - strand, within Pf1N1B4_166 at 206.915 kb on + strand, within Pf1N1B4_166 at 206.915 kb on + strand, within Pf1N1B4_166 at 206.915 kb on + strand, within Pf1N1B4_166 at 206.916 kb on - strand, within Pf1N1B4_166 at 206.916 kb on - strand, within Pf1N1B4_166 at 207.012 kb on - strand, within Pf1N1B4_166 at 207.041 kb on + strand, within Pf1N1B4_166
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 205,176 + Pf1N1B4_163 0.85 -0.4 205,315 + +0.4 205,353 - Pf1N1B4_164 0.11 +0.5 205,497 - Pf1N1B4_164 0.47 +0.4 205,497 - Pf1N1B4_164 0.47 +0.0 205,581 + Pf1N1B4_164 0.68 -1.2 205,582 - Pf1N1B4_164 0.68 -1.5 205,582 - Pf1N1B4_164 0.68 -0.0 205,670 + -1.2 205,670 + -0.8 205,706 + +0.6 205,706 + +0.4 205,706 + +2.4 205,706 + +0.7 205,707 - +0.9 205,707 - -1.8 206,094 + Pf1N1B4_165 0.69 +0.2 206,107 - Pf1N1B4_165 0.72 +0.8 206,160 + +1.4 206,160 + Pf1N1B4_165 0.87 +0.6 206,160 + Pf1N1B4_165 0.87 +1.2 206,160 + Pf1N1B4_165 0.87 -1.0 206,160 + Pf1N1B4_165 0.87 +2.1 206,160 + -0.2 206,160 + Pf1N1B4_165 0.87 +1.2 206,161 - +0.2 206,295 + +0.8 206,296 - +2.4 206,471 + Pf1N1B4_166 0.11 -1.2 206,507 + Pf1N1B4_166 0.14 -0.2 206,510 + Pf1N1B4_166 0.14 +0.8 206,510 + +0.4 206,510 + Pf1N1B4_166 0.14 +0.4 206,510 + Pf1N1B4_166 0.14 +0.2 206,510 + -0.5 206,511 - Pf1N1B4_166 0.14 -1.3 206,511 - Pf1N1B4_166 0.14 -0.2 206,561 + Pf1N1B4_166 0.19 -0.1 206,562 - Pf1N1B4_166 0.19 +1.6 206,630 + Pf1N1B4_166 0.25 +0.1 206,630 + Pf1N1B4_166 0.25 +1.6 206,631 - Pf1N1B4_166 0.25 +0.0 206,759 + Pf1N1B4_166 0.37 +0.8 206,783 + Pf1N1B4_166 0.39 +0.1 206,784 - -0.5 206,804 + Pf1N1B4_166 0.41 -2.5 206,804 + Pf1N1B4_166 0.41 -1.7 206,804 + -1.2 206,805 + Pf1N1B4_166 0.41 -1.2 206,805 - Pf1N1B4_166 0.41 +0.0 206,805 - Pf1N1B4_166 0.41 +0.3 206,805 - Pf1N1B4_166 0.41 +0.4 206,826 - Pf1N1B4_166 0.43 -0.2 206,826 - Pf1N1B4_166 0.43 -1.4 206,895 - Pf1N1B4_166 0.49 -1.2 206,915 + Pf1N1B4_166 0.50 -1.5 206,915 + Pf1N1B4_166 0.50 -1.8 206,915 + Pf1N1B4_166 0.50 +0.8 206,916 - Pf1N1B4_166 0.51 +0.6 206,916 - Pf1N1B4_166 0.51 -2.0 207,012 - Pf1N1B4_166 0.59 -0.4 207,041 + Pf1N1B4_166 0.62 -1.4
Or see this region's nucleotide sequence