Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_119 and Pf1N1B4_120 are separated by 92 nucleotides Pf1N1B4_120 and Pf1N1B4_121 are separated by 233 nucleotides
Pf1N1B4_119 - Putative haemolysin/cytolysin secreted via TPS pathway, at 125,607 to 130,118
_119
Pf1N1B4_120 - Channel-forming transporter/cytolysins activator of TpsB family, at 130,211 to 131,938
_120
Pf1N1B4_121 - Membrane fusion component of tripartite multidrug resistance system, at 132,172 to 133,293
_121
Position (kb)
130
131
132 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 129.286 kb on - strand, within Pf1N1B4_119 at 129.703 kb on + strand at 129.703 kb on + strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.704 kb on - strand at 129.892 kb on + strand at 129.893 kb on - strand at 129.893 kb on - strand at 130.134 kb on - strand at 130.291 kb on - strand at 130.456 kb on + strand at 130.569 kb on + strand, within Pf1N1B4_120 at 130.650 kb on + strand, within Pf1N1B4_120 at 130.734 kb on + strand, within Pf1N1B4_120 at 130.843 kb on - strand, within Pf1N1B4_120 at 131.020 kb on - strand, within Pf1N1B4_120 at 131.047 kb on - strand, within Pf1N1B4_120 at 131.050 kb on - strand, within Pf1N1B4_120 at 131.514 kb on + strand, within Pf1N1B4_120 at 131.514 kb on + strand, within Pf1N1B4_120 at 131.529 kb on + strand at 131.529 kb on + strand, within Pf1N1B4_120 at 131.529 kb on + strand, within Pf1N1B4_120 at 131.994 kb on - strand at 132.031 kb on - strand at 132.031 kb on - strand at 132.163 kb on + strand at 132.163 kb on + strand at 132.164 kb on - strand at 132.164 kb on - strand at 132.267 kb on + strand at 132.349 kb on + strand, within Pf1N1B4_121 at 132.349 kb on + strand, within Pf1N1B4_121 at 132.350 kb on - strand, within Pf1N1B4_121 at 132.524 kb on + strand, within Pf1N1B4_121 at 132.641 kb on - strand, within Pf1N1B4_121 at 132.856 kb on + strand, within Pf1N1B4_121
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 129,286 - Pf1N1B4_119 0.82 +0.2 129,703 + +0.1 129,703 + +0.8 129,704 - -0.5 129,704 - +0.4 129,704 - -2.2 129,704 - +0.2 129,704 - +0.8 129,892 + +0.1 129,893 - +0.4 129,893 - +0.0 130,134 - -0.8 130,291 - -1.8 130,456 + -0.2 130,569 + Pf1N1B4_120 0.21 -0.2 130,650 + Pf1N1B4_120 0.25 -0.5 130,734 + Pf1N1B4_120 0.30 -0.1 130,843 - Pf1N1B4_120 0.37 +1.8 131,020 - Pf1N1B4_120 0.47 +0.8 131,047 - Pf1N1B4_120 0.48 -1.0 131,050 - Pf1N1B4_120 0.49 +0.5 131,514 + Pf1N1B4_120 0.75 +0.6 131,514 + Pf1N1B4_120 0.75 -1.0 131,529 + -0.8 131,529 + Pf1N1B4_120 0.76 +1.1 131,529 + Pf1N1B4_120 0.76 +1.8 131,994 - +1.1 132,031 - +1.1 132,031 - +0.8 132,163 + -0.2 132,163 + +0.5 132,164 - -0.0 132,164 - -0.8 132,267 + +0.0 132,349 + Pf1N1B4_121 0.16 +1.2 132,349 + Pf1N1B4_121 0.16 +0.2 132,350 - Pf1N1B4_121 0.16 -0.4 132,524 + Pf1N1B4_121 0.31 +1.1 132,641 - Pf1N1B4_121 0.42 +0.5 132,856 + Pf1N1B4_121 0.61 +0.4
Or see this region's nucleotide sequence