Experiment: protonated control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hscB and iscA are separated by 95 nucleotides iscA and iscU are separated by 16 nucleotides iscU and iscS are separated by 27 nucleotides iscS and iscR are separated by 111 nucleotides
b2527: hscB - co-chaperone HscB (NCBI), at 2,656,974 to 2,657,489
hscB
b2528: iscA - iron-sulfur cluster assembly protein (NCBI), at 2,657,585 to 2,657,908
iscA
b2529: iscU - scaffold protein (NCBI), at 2,657,925 to 2,658,311
iscU
b2530: iscS - cysteine desulfurase (NCBI), at 2,658,339 to 2,659,553
iscS
b2531: iscR - DNA-binding transcriptional repressor (NCBI), at 2,659,665 to 2,660,153
iscR
Position (kb)
2658
2659
2660 Strain fitness (log2 ratio)
-2
-1
0
1 at 2657.633 kb on - strand, within iscA at 2657.795 kb on - strand, within iscA at 2657.873 kb on - strand, within iscA at 2657.886 kb on - strand at 2658.370 kb on + strand at 2659.636 kb on - strand at 2659.636 kb on - strand at 2659.684 kb on - strand at 2659.834 kb on - strand, within iscR at 2659.875 kb on - strand, within iscR at 2659.897 kb on - strand, within iscR at 2660.133 kb on - strand at 2660.376 kb on + strand at 2660.376 kb on + strand at 2660.376 kb on + strand at 2660.376 kb on + strand at 2660.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction protonated control remove 2,657,633 - iscA b2528 0.15 -1.9 2,657,795 - iscA b2528 0.65 +0.1 2,657,873 - iscA b2528 0.89 +0.3 2,657,886 - +0.8 2,658,370 + +1.2 2,659,636 - -0.4 2,659,636 - +0.8 2,659,684 - -1.9 2,659,834 - iscR b2531 0.35 -0.4 2,659,875 - iscR b2531 0.43 -0.4 2,659,897 - iscR b2531 0.47 +0.3 2,660,133 - -0.2 2,660,376 + -0.2 2,660,376 + +0.0 2,660,376 + +1.2 2,660,376 + +0.1 2,660,475 - -0.7
Or see this region's nucleotide sequence