Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2465

Experiment: KB with Mitomycin C 0.625 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2464 and Psyr_2465 are separated by 406 nucleotidesPsyr_2465 and Psyr_2466 are separated by 70 nucleotidesPsyr_2466 and Psyr_2467 are separated by 96 nucleotides Psyr_2464: Psyr_2464 - methionine synthase (B12-dependent), at 2,852,906 to 2,856,625 _2464 Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054 _2465 Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718 _2466 Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764 _2467 Position (kb) 2857 2858 2859Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2856.688 kb on + strandat 2856.746 kb on + strandat 2856.746 kb on + strandat 2856.747 kb on - strandat 2856.747 kb on - strandat 2856.754 kb on + strandat 2856.754 kb on + strandat 2856.754 kb on + strandat 2856.754 kb on + strandat 2856.802 kb on - strandat 2856.924 kb on + strandat 2856.975 kb on + strandat 2856.975 kb on + strandat 2857.014 kb on + strandat 2857.014 kb on + strandat 2857.015 kb on - strandat 2857.278 kb on + strand, within Psyr_2465at 2857.286 kb on - strand, within Psyr_2465at 2857.287 kb on + strand, within Psyr_2465at 2857.287 kb on + strand, within Psyr_2465at 2857.287 kb on + strand, within Psyr_2465at 2857.287 kb on + strand, within Psyr_2465at 2857.288 kb on - strand, within Psyr_2465at 2857.449 kb on + strand, within Psyr_2465at 2857.449 kb on + strand, within Psyr_2465at 2857.449 kb on + strand, within Psyr_2465at 2857.449 kb on + strand, within Psyr_2465at 2857.449 kb on + strand, within Psyr_2465at 2857.450 kb on - strand, within Psyr_2465at 2857.450 kb on - strand, within Psyr_2465at 2857.450 kb on - strand, within Psyr_2465at 2857.464 kb on + strand, within Psyr_2465at 2857.465 kb on - strand, within Psyr_2465at 2857.465 kb on - strand, within Psyr_2465at 2857.465 kb on - strand, within Psyr_2465at 2857.483 kb on + strand, within Psyr_2465at 2857.483 kb on + strand, within Psyr_2465at 2857.484 kb on - strand, within Psyr_2465at 2857.484 kb on - strand, within Psyr_2465at 2857.599 kb on + strand, within Psyr_2465at 2857.600 kb on - strand, within Psyr_2465at 2857.600 kb on - strand, within Psyr_2465at 2857.600 kb on - strand, within Psyr_2465at 2857.699 kb on - strand, within Psyr_2465at 2857.791 kb on + strand, within Psyr_2465at 2857.836 kb on + strand, within Psyr_2465at 2857.845 kb on + strand, within Psyr_2465at 2857.912 kb on - strand, within Psyr_2465at 2857.912 kb on - strand, within Psyr_2465at 2857.912 kb on - strand, within Psyr_2465at 2857.965 kb on + strandat 2857.965 kb on + strandat 2857.966 kb on - strandat 2858.713 kb on + strandat 2858.713 kb on + strandat 2858.713 kb on + strandat 2858.713 kb on + strandat 2858.714 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml
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2,856,688 + +0.1
2,856,746 + +3.1
2,856,746 + +0.2
2,856,747 - -4.3
2,856,747 - -4.0
2,856,754 + +2.7
2,856,754 + -1.4
2,856,754 + -2.3
2,856,754 + +2.4
2,856,802 - -1.6
2,856,924 + +1.1
2,856,975 + -3.3
2,856,975 + -4.2
2,857,014 + -0.6
2,857,014 + -1.5
2,857,015 - -3.1
2,857,278 + Psyr_2465 0.24 +0.1
2,857,286 - Psyr_2465 0.25 +0.1
2,857,287 + Psyr_2465 0.25 -0.4
2,857,287 + Psyr_2465 0.25 +0.1
2,857,287 + Psyr_2465 0.25 +1.1
2,857,287 + Psyr_2465 0.25 +1.1
2,857,288 - Psyr_2465 0.25 +0.1
2,857,449 + Psyr_2465 0.41 -2.8
2,857,449 + Psyr_2465 0.41 -3.5
2,857,449 + Psyr_2465 0.41 -3.7
2,857,449 + Psyr_2465 0.41 +2.9
2,857,449 + Psyr_2465 0.41 +3.5
2,857,450 - Psyr_2465 0.41 +3.1
2,857,450 - Psyr_2465 0.41 +3.2
2,857,450 - Psyr_2465 0.41 -0.6
2,857,464 + Psyr_2465 0.42 -1.8
2,857,465 - Psyr_2465 0.42 +1.1
2,857,465 - Psyr_2465 0.42 -3.6
2,857,465 - Psyr_2465 0.42 +0.1
2,857,483 + Psyr_2465 0.44 +2.9
2,857,483 + Psyr_2465 0.44 -0.5
2,857,484 - Psyr_2465 0.44 +0.1
2,857,484 - Psyr_2465 0.44 -3.6
2,857,599 + Psyr_2465 0.55 -0.9
2,857,600 - Psyr_2465 0.56 +0.1
2,857,600 - Psyr_2465 0.56 -3.1
2,857,600 - Psyr_2465 0.56 -2.2
2,857,699 - Psyr_2465 0.65 -1.7
2,857,791 + Psyr_2465 0.74 +2.9
2,857,836 + Psyr_2465 0.79 +1.7
2,857,845 + Psyr_2465 0.79 +0.1
2,857,912 - Psyr_2465 0.86 -1.7
2,857,912 - Psyr_2465 0.86 +0.1
2,857,912 - Psyr_2465 0.86 -3.9
2,857,965 + -1.2
2,857,965 + -0.3
2,857,966 - -2.3
2,858,713 + -0.2
2,858,713 + +3.3
2,858,713 + +0.1
2,858,713 + -3.3
2,858,714 - -0.2

Or see this region's nucleotide sequence