Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2464 and Psyr_2465 are separated by 406 nucleotides Psyr_2465 and Psyr_2466 are separated by 70 nucleotides Psyr_2466 and Psyr_2467 are separated by 96 nucleotides
Psyr_2464: Psyr_2464 - methionine synthase (B12-dependent), at 2,852,906 to 2,856,625
_2464
Psyr_2465: Psyr_2465 - Acyltransferase 3, at 2,857,032 to 2,858,054
_2465
Psyr_2466: Psyr_2466 - HesB/YadR/YfhF:Nitrogen-fixing NifU, C-terminal, at 2,858,125 to 2,858,718
_2466
Psyr_2467: Psyr_2467 - 3-methylcrotonoyl-CoA carboxylase, alpha subunit, at 2,858,815 to 2,860,764
_2467
Position (kb)
2857
2858
2859 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2856.688 kb on + strand at 2856.746 kb on + strand at 2856.746 kb on + strand at 2856.747 kb on - strand at 2856.747 kb on - strand at 2856.754 kb on + strand at 2856.754 kb on + strand at 2856.754 kb on + strand at 2856.754 kb on + strand at 2856.802 kb on - strand at 2856.924 kb on + strand at 2856.975 kb on + strand at 2856.975 kb on + strand at 2857.014 kb on + strand at 2857.014 kb on + strand at 2857.015 kb on - strand at 2857.278 kb on + strand, within Psyr_2465 at 2857.286 kb on - strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.287 kb on + strand, within Psyr_2465 at 2857.288 kb on - strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.449 kb on + strand, within Psyr_2465 at 2857.450 kb on - strand, within Psyr_2465 at 2857.450 kb on - strand, within Psyr_2465 at 2857.450 kb on - strand, within Psyr_2465 at 2857.464 kb on + strand, within Psyr_2465 at 2857.465 kb on - strand, within Psyr_2465 at 2857.465 kb on - strand, within Psyr_2465 at 2857.465 kb on - strand, within Psyr_2465 at 2857.483 kb on + strand, within Psyr_2465 at 2857.483 kb on + strand, within Psyr_2465 at 2857.484 kb on - strand, within Psyr_2465 at 2857.484 kb on - strand, within Psyr_2465 at 2857.599 kb on + strand, within Psyr_2465 at 2857.600 kb on - strand, within Psyr_2465 at 2857.600 kb on - strand, within Psyr_2465 at 2857.600 kb on - strand, within Psyr_2465 at 2857.699 kb on - strand, within Psyr_2465 at 2857.791 kb on + strand, within Psyr_2465 at 2857.836 kb on + strand, within Psyr_2465 at 2857.845 kb on + strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.912 kb on - strand, within Psyr_2465 at 2857.965 kb on + strand at 2857.965 kb on + strand at 2857.966 kb on - strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.713 kb on + strand at 2858.714 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 2,856,688 + +0.1 2,856,746 + +3.1 2,856,746 + +0.2 2,856,747 - -4.3 2,856,747 - -4.0 2,856,754 + +2.7 2,856,754 + -1.4 2,856,754 + -2.3 2,856,754 + +2.4 2,856,802 - -1.6 2,856,924 + +1.1 2,856,975 + -3.3 2,856,975 + -4.2 2,857,014 + -0.6 2,857,014 + -1.5 2,857,015 - -3.1 2,857,278 + Psyr_2465 0.24 +0.1 2,857,286 - Psyr_2465 0.25 +0.1 2,857,287 + Psyr_2465 0.25 -0.4 2,857,287 + Psyr_2465 0.25 +0.1 2,857,287 + Psyr_2465 0.25 +1.1 2,857,287 + Psyr_2465 0.25 +1.1 2,857,288 - Psyr_2465 0.25 +0.1 2,857,449 + Psyr_2465 0.41 -2.8 2,857,449 + Psyr_2465 0.41 -3.5 2,857,449 + Psyr_2465 0.41 -3.7 2,857,449 + Psyr_2465 0.41 +2.9 2,857,449 + Psyr_2465 0.41 +3.5 2,857,450 - Psyr_2465 0.41 +3.1 2,857,450 - Psyr_2465 0.41 +3.2 2,857,450 - Psyr_2465 0.41 -0.6 2,857,464 + Psyr_2465 0.42 -1.8 2,857,465 - Psyr_2465 0.42 +1.1 2,857,465 - Psyr_2465 0.42 -3.6 2,857,465 - Psyr_2465 0.42 +0.1 2,857,483 + Psyr_2465 0.44 +2.9 2,857,483 + Psyr_2465 0.44 -0.5 2,857,484 - Psyr_2465 0.44 +0.1 2,857,484 - Psyr_2465 0.44 -3.6 2,857,599 + Psyr_2465 0.55 -0.9 2,857,600 - Psyr_2465 0.56 +0.1 2,857,600 - Psyr_2465 0.56 -3.1 2,857,600 - Psyr_2465 0.56 -2.2 2,857,699 - Psyr_2465 0.65 -1.7 2,857,791 + Psyr_2465 0.74 +2.9 2,857,836 + Psyr_2465 0.79 +1.7 2,857,845 + Psyr_2465 0.79 +0.1 2,857,912 - Psyr_2465 0.86 -1.7 2,857,912 - Psyr_2465 0.86 +0.1 2,857,912 - Psyr_2465 0.86 -3.9 2,857,965 + -1.2 2,857,965 + -0.3 2,857,966 - -2.3 2,858,713 + -0.2 2,858,713 + +3.3 2,858,713 + +0.1 2,858,713 + -3.3 2,858,714 - -0.2
Or see this region's nucleotide sequence