Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0449 and Psyr_0450 are separated by 1 nucleotides Psyr_0450 and Psyr_0451 are separated by 1 nucleotides Psyr_0451 and Psyr_0452 are separated by 122 nucleotides Psyr_0452 and Psyr_0453 are separated by 106 nucleotides
Psyr_0449: Psyr_0449 - Malonate transporter MadL subunit, at 485,743 to 486,159
_0449
Psyr_0450: Psyr_0450 - Malonate/sodium symporter MadM subunit, at 486,161 to 486,925
_0450
Psyr_0451: Psyr_0451 - regulatory protein, LysR:LysR, substrate-binding protein, at 486,927 to 487,853
_0451
Psyr_0452: Psyr_0452 - HylII, at 487,976 to 488,590
_0452
Psyr_0453: Psyr_0453 - 16S rRNA m(3)U-1498 methyltransferase, at 488,697 to 489,416
_0453
Position (kb) 486
487
488 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 485.953 kb on + strand, within Psyr_0449 at 485.953 kb on + strand, within Psyr_0449 at 485.953 kb on + strand, within Psyr_0449 at 485.953 kb on + strand, within Psyr_0449 at 485.953 kb on + strand, within Psyr_0449 at 485.953 kb on + strand, within Psyr_0449 at 485.954 kb on - strand, within Psyr_0449 at 485.954 kb on - strand, within Psyr_0449 at 485.954 kb on - strand, within Psyr_0449 at 485.954 kb on - strand, within Psyr_0449 at 486.160 kb on + strand at 486.160 kb on + strand at 486.160 kb on + strand at 486.160 kb on + strand at 486.161 kb on - strand at 486.161 kb on - strand at 486.161 kb on - strand at 486.499 kb on - strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.602 kb on + strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.603 kb on - strand, within Psyr_0450 at 486.651 kb on + strand, within Psyr_0450 at 486.651 kb on + strand, within Psyr_0450 at 486.652 kb on - strand, within Psyr_0450 at 486.652 kb on - strand, within Psyr_0450 at 486.652 kb on - strand, within Psyr_0450 at 486.652 kb on - strand, within Psyr_0450 at 486.652 kb on - strand, within Psyr_0450 at 486.652 kb on - strand, within Psyr_0450 at 486.896 kb on + strand at 486.896 kb on + strand at 486.896 kb on + strand at 486.896 kb on + strand at 486.897 kb on - strand at 486.897 kb on - strand at 486.897 kb on - strand at 486.897 kb on - strand at 486.924 kb on - strand at 486.958 kb on + strand at 486.958 kb on + strand at 486.958 kb on + strand at 486.958 kb on + strand at 486.959 kb on - strand at 486.959 kb on - strand at 486.959 kb on - strand at 486.959 kb on - strand at 486.959 kb on - strand at 487.435 kb on + strand, within Psyr_0451 at 487.435 kb on + strand, within Psyr_0451 at 487.435 kb on + strand, within Psyr_0451 at 487.436 kb on - strand, within Psyr_0451 at 487.436 kb on - strand, within Psyr_0451 at 487.436 kb on - strand, within Psyr_0451 at 487.541 kb on - strand, within Psyr_0451 at 487.541 kb on - strand, within Psyr_0451 at 487.651 kb on + strand, within Psyr_0451 at 487.651 kb on + strand, within Psyr_0451 at 487.651 kb on + strand, within Psyr_0451 at 487.651 kb on + strand, within Psyr_0451 at 487.652 kb on - strand, within Psyr_0451 at 487.652 kb on - strand, within Psyr_0451 at 487.652 kb on - strand, within Psyr_0451 at 487.652 kb on - strand, within Psyr_0451 at 487.652 kb on - strand, within Psyr_0451 at 487.652 kb on - strand, within Psyr_0451 at 487.752 kb on - strand, within Psyr_0451 at 487.752 kb on - strand, within Psyr_0451 at 487.813 kb on - strand at 487.813 kb on - strand at 487.865 kb on + strand at 487.872 kb on - strand at 487.872 kb on - strand at 487.894 kb on + strand at 487.894 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.904 kb on + strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.905 kb on - strand at 487.947 kb on + strand at 487.947 kb on + strand at 487.947 kb on + strand at 487.948 kb on - strand at 487.956 kb on + strand at 487.969 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.979 kb on + strand at 487.980 kb on - strand at 487.980 kb on - strand at 487.980 kb on - strand at 487.980 kb on - strand at 487.982 kb on + strand at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.108 kb on + strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.109 kb on - strand, within Psyr_0452 at 488.132 kb on + strand, within Psyr_0452 at 488.133 kb on - strand, within Psyr_0452 at 488.133 kb on - strand, within Psyr_0452 at 488.133 kb on - strand, within Psyr_0452 at 488.150 kb on + strand, within Psyr_0452 at 488.210 kb on + strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.211 kb on - strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.231 kb on + strand, within Psyr_0452 at 488.232 kb on - strand, within Psyr_0452 at 488.232 kb on - strand, within Psyr_0452 at 488.328 kb on + strand, within Psyr_0452 at 488.328 kb on + strand, within Psyr_0452 at 488.329 kb on - strand, within Psyr_0452 at 488.369 kb on + strand, within Psyr_0452 at 488.370 kb on - strand, within Psyr_0452 at 488.370 kb on - strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.468 kb on + strand, within Psyr_0452 at 488.469 kb on - strand, within Psyr_0452 at 488.579 kb on + strand at 488.579 kb on + strand at 488.579 kb on + strand at 488.580 kb on - strand at 488.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 485,953 + Psyr_0449 0.50 +2.2 485,953 + Psyr_0449 0.50 -0.3 485,953 + Psyr_0449 0.50 +0.9 485,953 + Psyr_0449 0.50 -3.0 485,953 + Psyr_0449 0.50 +1.5 485,953 + Psyr_0449 0.50 -0.3 485,954 - Psyr_0449 0.51 -0.4 485,954 - Psyr_0449 0.51 -0.5 485,954 - Psyr_0449 0.51 -0.8 485,954 - Psyr_0449 0.51 -1.5 486,160 + -0.9 486,160 + -0.9 486,160 + -1.8 486,160 + +3.5 486,161 - +2.3 486,161 - -1.9 486,161 - -3.5 486,499 - Psyr_0450 0.44 +0.3 486,602 + Psyr_0450 0.58 -1.3 486,602 + Psyr_0450 0.58 +3.1 486,602 + Psyr_0450 0.58 -0.3 486,602 + Psyr_0450 0.58 +2.2 486,602 + Psyr_0450 0.58 +2.5 486,602 + Psyr_0450 0.58 -3.1 486,602 + Psyr_0450 0.58 +0.3 486,602 + Psyr_0450 0.58 +1.2 486,602 + Psyr_0450 0.58 -0.6 486,602 + Psyr_0450 0.58 -0.4 486,602 + Psyr_0450 0.58 -1.5 486,602 + Psyr_0450 0.58 +4.1 486,602 + Psyr_0450 0.58 -0.7 486,602 + Psyr_0450 0.58 +0.6 486,603 - Psyr_0450 0.58 +0.1 486,603 - Psyr_0450 0.58 -1.2 486,603 - Psyr_0450 0.58 -0.1 486,603 - Psyr_0450 0.58 +0.9 486,603 - Psyr_0450 0.58 +1.5 486,603 - Psyr_0450 0.58 -0.9 486,603 - Psyr_0450 0.58 +2.5 486,603 - Psyr_0450 0.58 -0.5 486,651 + Psyr_0450 0.64 +1.5 486,651 + Psyr_0450 0.64 -1.9 486,652 - Psyr_0450 0.64 +1.2 486,652 - Psyr_0450 0.64 -1.2 486,652 - Psyr_0450 0.64 -0.1 486,652 - Psyr_0450 0.64 +1.3 486,652 - Psyr_0450 0.64 -1.7 486,652 - Psyr_0450 0.64 -3.1 486,896 + -2.0 486,896 + -1.6 486,896 + -2.3 486,896 + +0.6 486,897 - -2.7 486,897 - -1.7 486,897 - -2.5 486,897 - -0.7 486,924 - -2.7 486,958 + +3.2 486,958 + -2.1 486,958 + -3.1 486,958 + +0.4 486,959 - -0.8 486,959 - -0.8 486,959 - +3.6 486,959 - -1.2 486,959 - -1.0 487,435 + Psyr_0451 0.55 +3.2 487,435 + Psyr_0451 0.55 -1.4 487,435 + Psyr_0451 0.55 +0.7 487,436 - Psyr_0451 0.55 -3.0 487,436 - Psyr_0451 0.55 -0.1 487,436 - Psyr_0451 0.55 -3.1 487,541 - Psyr_0451 0.66 -2.5 487,541 - Psyr_0451 0.66 -0.8 487,651 + Psyr_0451 0.78 -1.4 487,651 + Psyr_0451 0.78 -1.3 487,651 + Psyr_0451 0.78 -0.5 487,651 + Psyr_0451 0.78 -2.5 487,652 - Psyr_0451 0.78 +1.5 487,652 - Psyr_0451 0.78 +2.5 487,652 - Psyr_0451 0.78 +2.9 487,652 - Psyr_0451 0.78 +4.1 487,652 - Psyr_0451 0.78 +0.9 487,652 - Psyr_0451 0.78 -2.9 487,752 - Psyr_0451 0.89 -1.7 487,752 - Psyr_0451 0.89 +2.5 487,813 - -2.6 487,813 - -1.7 487,865 + +0.7 487,872 - +0.9 487,872 - +3.4 487,894 + -1.2 487,894 + +4.2 487,904 + +2.5 487,904 + -1.7 487,904 + +0.6 487,904 + +2.2 487,904 + -0.1 487,904 + +3.6 487,904 + +3.8 487,904 + -2.5 487,904 + +2.2 487,905 - -4.0 487,905 - -1.2 487,905 - -1.2 487,905 - +3.4 487,905 - -1.2 487,905 - -0.9 487,905 - -4.4 487,905 - -0.4 487,905 - -2.8 487,905 - +3.2 487,905 - -1.6 487,905 - +4.0 487,905 - +2.2 487,905 - -3.8 487,947 + +0.6 487,947 + +1.8 487,947 + -1.7 487,948 - -4.5 487,956 + +3.9 487,969 + -0.3 487,979 + +4.3 487,979 + -0.4 487,979 + -0.7 487,979 + +0.6 487,979 + +2.7 487,979 + +0.1 487,979 + +2.7 487,980 - -0.1 487,980 - -3.5 487,980 - -0.3 487,980 - -2.1 487,982 + +4.2 488,108 + Psyr_0452 0.21 -2.2 488,108 + Psyr_0452 0.21 -4.7 488,108 + Psyr_0452 0.21 -0.8 488,108 + Psyr_0452 0.21 +0.1 488,108 + Psyr_0452 0.21 -5.0 488,108 + Psyr_0452 0.21 -0.1 488,108 + Psyr_0452 0.21 +3.1 488,108 + Psyr_0452 0.21 +2.4 488,108 + Psyr_0452 0.21 -2.9 488,108 + Psyr_0452 0.21 +3.9 488,108 + Psyr_0452 0.21 +3.6 488,108 + Psyr_0452 0.21 +2.2 488,109 - Psyr_0452 0.22 -0.1 488,109 - Psyr_0452 0.22 -1.6 488,109 - Psyr_0452 0.22 -0.4 488,109 - Psyr_0452 0.22 -2.3 488,109 - Psyr_0452 0.22 -0.7 488,109 - Psyr_0452 0.22 -2.2 488,109 - Psyr_0452 0.22 -0.7 488,109 - Psyr_0452 0.22 +1.9 488,109 - Psyr_0452 0.22 -0.9 488,109 - Psyr_0452 0.22 -0.3 488,109 - Psyr_0452 0.22 +0.9 488,109 - Psyr_0452 0.22 -3.7 488,109 - Psyr_0452 0.22 -1.8 488,109 - Psyr_0452 0.22 -3.4 488,109 - Psyr_0452 0.22 +0.9 488,109 - Psyr_0452 0.22 -0.1 488,109 - Psyr_0452 0.22 -0.1 488,132 + Psyr_0452 0.25 -0.1 488,133 - Psyr_0452 0.26 -1.4 488,133 - Psyr_0452 0.26 +0.9 488,133 - Psyr_0452 0.26 -1.8 488,150 + Psyr_0452 0.28 +4.2 488,210 + Psyr_0452 0.38 +1.9 488,211 - Psyr_0452 0.38 -0.1 488,211 - Psyr_0452 0.38 -2.3 488,211 - Psyr_0452 0.38 -2.2 488,211 - Psyr_0452 0.38 -1.6 488,211 - Psyr_0452 0.38 -5.2 488,231 + Psyr_0452 0.41 -2.1 488,231 + Psyr_0452 0.41 -3.4 488,231 + Psyr_0452 0.41 +2.9 488,231 + Psyr_0452 0.41 +3.4 488,232 - Psyr_0452 0.42 +2.2 488,232 - Psyr_0452 0.42 -0.1 488,328 + Psyr_0452 0.57 -2.7 488,328 + Psyr_0452 0.57 -2.8 488,329 - Psyr_0452 0.57 -4.1 488,369 + Psyr_0452 0.64 -1.0 488,370 - Psyr_0452 0.64 +2.2 488,370 - Psyr_0452 0.64 -2.5 488,468 + Psyr_0452 0.80 +4.1 488,468 + Psyr_0452 0.80 -0.1 488,468 + Psyr_0452 0.80 -0.9 488,468 + Psyr_0452 0.80 -3.2 488,469 - Psyr_0452 0.80 -0.1 488,579 + +2.4 488,579 + -1.5 488,579 + +1.5 488,580 - +1.9 488,580 - +1.5
Or see this region's nucleotide sequence