Experiment: KB with Mitomycin C 0.625 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0012 and Psyr_0013 are separated by 20 nucleotides Psyr_0013 and Psyr_0014 are separated by 38 nucleotides
Psyr_0012: Psyr_0012 - glycyl-tRNA synthetase alpha chain, at 13,967 to 14,974
_0012
Psyr_0013: Psyr_0013 - DNA-3-methyladenine glycosylase I, at 14,995 to 15,546
_0013
Psyr_0014: Psyr_0014 - lipid A biosynthesis acyltransferase, at 15,585 to 16,472
_0014
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 14.956 kb on - strand at 14.956 kb on - strand at 14.994 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.031 kb on + strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.032 kb on - strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.040 kb on + strand at 15.041 kb on - strand at 15.041 kb on - strand at 15.170 kb on + strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.171 kb on - strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.475 kb on + strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.476 kb on - strand, within Psyr_0013 at 15.591 kb on - strand at 15.975 kb on + strand, within Psyr_0014 at 15.975 kb on + strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.335 kb on + strand, within Psyr_0014 at 16.335 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.389 kb on + strand at 16.389 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.414 kb on - strand at 16.414 kb on - strand at 16.467 kb on + strand at 16.467 kb on + strand at 16.470 kb on + strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.625 ug/ml remove 14,956 - -4.2 14,956 - +2.2 14,994 + -1.6 15,031 + +0.9 15,031 + +0.5 15,031 + -1.8 15,032 - -0.9 15,032 - -1.0 15,032 - -2.5 15,032 - +4.8 15,032 - +1.5 15,032 - +2.2 15,032 - +2.5 15,032 - -4.3 15,032 - +1.9 15,040 + -1.4 15,040 + +1.6 15,040 + -0.4 15,040 + +1.3 15,041 - -2.7 15,041 - -2.5 15,170 + Psyr_0013 0.32 -0.4 15,171 - Psyr_0013 0.32 +0.9 15,171 - Psyr_0013 0.32 -2.9 15,171 - Psyr_0013 0.32 +2.2 15,475 + Psyr_0013 0.87 -0.5 15,475 + Psyr_0013 0.87 -1.8 15,475 + Psyr_0013 0.87 +0.7 15,475 + Psyr_0013 0.87 -0.3 15,475 + Psyr_0013 0.87 -0.3 15,475 + Psyr_0013 0.87 -1.1 15,475 + Psyr_0013 0.87 -0.5 15,476 - Psyr_0013 0.87 -2.3 15,476 - Psyr_0013 0.87 +2.7 15,591 - -4.5 15,975 + Psyr_0014 0.44 -0.1 15,975 + Psyr_0014 0.44 -4.7 16,302 - Psyr_0014 0.81 -5.2 16,302 - Psyr_0014 0.81 -1.0 16,302 - Psyr_0014 0.81 -6.0 16,335 + Psyr_0014 0.84 -2.9 16,335 - Psyr_0014 0.84 -0.1 16,336 - Psyr_0014 0.85 -0.1 16,336 - Psyr_0014 0.85 -0.1 16,336 - Psyr_0014 0.85 -4.9 16,336 - Psyr_0014 0.85 -0.1 16,336 - Psyr_0014 0.85 -2.7 16,336 - Psyr_0014 0.85 -4.5 16,389 + -3.5 16,389 + -3.5 16,413 + -2.7 16,413 + -0.1 16,413 + -3.9 16,413 + -0.1 16,414 - -3.4 16,414 - -5.3 16,467 + -0.7 16,467 + -1.0 16,470 + -4.2 16,471 - +2.5 16,471 - -2.0 16,471 - -3.6
Or see this region's nucleotide sequence