Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_613 and Pf1N1B4_614 are separated by 446 nucleotides Pf1N1B4_614 and Pf1N1B4_615 are separated by 35 nucleotides
Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306
_613
Pf1N1B4_614 - Arginine/ornithine antiporter ArcD, at 709,753 to 711,180
_614
Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472
_615
Position (kb) 709
710
711
712 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand at 709.083 kb on + strand at 709.083 kb on + strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand at 709.084 kb on - strand, within Pf1N1B4_613 at 709.335 kb on - strand at 709.374 kb on - strand at 709.385 kb on - strand at 709.422 kb on - strand at 709.425 kb on + strand at 709.426 kb on - strand at 709.473 kb on + strand at 709.473 kb on + strand at 709.474 kb on - strand at 709.529 kb on + strand at 709.540 kb on + strand at 709.541 kb on - strand at 709.541 kb on - strand at 709.643 kb on + strand at 709.643 kb on + strand at 709.652 kb on - strand at 709.882 kb on - strand at 709.976 kb on - strand, within Pf1N1B4_614 at 710.005 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand at 710.816 kb on - strand, within Pf1N1B4_614 at 710.830 kb on + strand, within Pf1N1B4_614 at 710.861 kb on - strand, within Pf1N1B4_614 at 710.911 kb on + strand, within Pf1N1B4_614 at 710.912 kb on - strand at 710.912 kb on - strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.990 kb on - strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.123 kb on - strand at 711.123 kb on - strand at 711.214 kb on + strand at 711.237 kb on + strand at 711.756 kb on + strand, within Pf1N1B4_615 at 711.757 kb on - strand, within Pf1N1B4_615
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 709,054 - Pf1N1B4_613 0.82 -1.7 709,054 - Pf1N1B4_613 0.82 -0.7 709,054 - Pf1N1B4_613 0.82 -1.7 709,054 - Pf1N1B4_613 0.82 -0.9 709,083 + Pf1N1B4_613 0.84 +0.3 709,083 + Pf1N1B4_613 0.84 +0.5 709,083 + -0.4 709,083 + -0.4 709,083 + Pf1N1B4_613 0.84 -0.9 709,084 - Pf1N1B4_613 0.84 +0.9 709,084 - Pf1N1B4_613 0.84 +0.3 709,084 - Pf1N1B4_613 0.84 +0.2 709,084 - Pf1N1B4_613 0.84 -2.4 709,084 - Pf1N1B4_613 0.84 -0.2 709,084 - +2.5 709,084 - Pf1N1B4_613 0.84 -1.4 709,335 - -0.7 709,374 - +1.2 709,385 - +0.8 709,422 - +0.2 709,425 + -1.1 709,426 - -0.1 709,473 + +0.9 709,473 + -0.9 709,474 - -0.1 709,529 + +0.9 709,540 + -1.4 709,541 - -0.1 709,541 - +1.1 709,643 + -0.4 709,643 + +0.9 709,652 - +0.0 709,882 - -1.7 709,976 - Pf1N1B4_614 0.16 -0.7 710,005 + Pf1N1B4_614 0.18 +0.2 710,065 + Pf1N1B4_614 0.22 +0.0 710,065 + +0.9 710,816 - Pf1N1B4_614 0.74 +1.9 710,830 + Pf1N1B4_614 0.75 +0.3 710,861 - Pf1N1B4_614 0.78 +0.3 710,911 + Pf1N1B4_614 0.81 +0.7 710,912 - +2.9 710,912 - Pf1N1B4_614 0.81 -0.7 710,971 + Pf1N1B4_614 0.85 +1.6 710,971 + Pf1N1B4_614 0.85 +0.9 710,990 - Pf1N1B4_614 0.87 +0.9 711,031 + Pf1N1B4_614 0.89 +0.9 711,031 + Pf1N1B4_614 0.89 +1.2 711,123 - -1.1 711,123 - +1.2 711,214 + -0.7 711,237 + -1.1 711,756 + Pf1N1B4_615 0.43 -1.1 711,757 - Pf1N1B4_615 0.43 +1.3
Or see this region's nucleotide sequence