Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2253 and Pf1N1B4_2254 are separated by 227 nucleotides Pf1N1B4_2254 and Pf1N1B4_2255 are separated by 374 nucleotides
Pf1N1B4_2253 - Transcriptional regulator, TetR family, at 2,568,719 to 2,569,351
_2253
Pf1N1B4_2254 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 2,569,579 to 2,570,859
_2254
Pf1N1B4_2255 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94), at 2,571,234 to 2,572,010
_2255
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2568.741 kb on + strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.742 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.754 kb on - strand at 2568.765 kb on + strand at 2568.765 kb on + strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.766 kb on - strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.840 kb on + strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2568.841 kb on - strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.035 kb on + strand, within Pf1N1B4_2253 at 2569.036 kb on - strand, within Pf1N1B4_2253 at 2569.233 kb on + strand, within Pf1N1B4_2253 at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.381 kb on + strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.382 kb on - strand at 2569.429 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.464 kb on + strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.465 kb on - strand at 2569.494 kb on - strand at 2569.524 kb on + strand at 2569.524 kb on + strand at 2569.545 kb on + strand at 2569.580 kb on + strand at 2569.595 kb on + strand at 2569.596 kb on - strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.604 kb on + strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.605 kb on - strand at 2569.654 kb on - strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.676 kb on + strand at 2569.677 kb on - strand at 2569.677 kb on - strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.691 kb on + strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.692 kb on - strand at 2569.772 kb on + strand, within Pf1N1B4_2254 at 2569.772 kb on + strand, within Pf1N1B4_2254 at 2569.773 kb on - strand, within Pf1N1B4_2254 at 2569.773 kb on - strand, within Pf1N1B4_2254 at 2569.786 kb on + strand, within Pf1N1B4_2254 at 2569.786 kb on + strand, within Pf1N1B4_2254 at 2569.787 kb on - strand, within Pf1N1B4_2254 at 2569.942 kb on - strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.979 kb on + strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2569.980 kb on - strand, within Pf1N1B4_2254 at 2570.003 kb on + strand, within Pf1N1B4_2254 at 2570.130 kb on + strand, within Pf1N1B4_2254 at 2570.220 kb on - strand, within Pf1N1B4_2254 at 2570.397 kb on - strand, within Pf1N1B4_2254 at 2570.397 kb on - strand, within Pf1N1B4_2254 at 2570.397 kb on - strand, within Pf1N1B4_2254 at 2570.420 kb on + strand, within Pf1N1B4_2254 at 2570.421 kb on - strand, within Pf1N1B4_2254 at 2570.507 kb on + strand, within Pf1N1B4_2254 at 2570.508 kb on - strand, within Pf1N1B4_2254 at 2570.508 kb on - strand, within Pf1N1B4_2254 at 2570.632 kb on + strand, within Pf1N1B4_2254 at 2570.633 kb on - strand, within Pf1N1B4_2254 at 2570.761 kb on - strand at 2570.773 kb on + strand at 2570.773 kb on + strand at 2570.773 kb on + strand at 2570.774 kb on - strand at 2570.774 kb on - strand at 2570.774 kb on - strand at 2570.805 kb on - strand at 2570.805 kb on - strand at 2570.859 kb on + strand at 2570.882 kb on + strand at 2570.883 kb on - strand at 2570.883 kb on - strand at 2570.982 kb on - strand at 2571.201 kb on - strand at 2571.217 kb on + strand at 2571.217 kb on + strand at 2571.218 kb on - strand at 2571.218 kb on - strand at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.315 kb on + strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.316 kb on - strand, within Pf1N1B4_2255 at 2571.319 kb on + strand, within Pf1N1B4_2255 at 2571.320 kb on - strand, within Pf1N1B4_2255 at 2571.379 kb on + strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.380 kb on - strand, within Pf1N1B4_2255 at 2571.440 kb on + strand, within Pf1N1B4_2255 at 2571.440 kb on + strand, within Pf1N1B4_2255 at 2571.440 kb on + strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.441 kb on - strand, within Pf1N1B4_2255 at 2571.443 kb on + strand at 2571.443 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.445 kb on + strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.446 kb on - strand, within Pf1N1B4_2255 at 2571.459 kb on + strand, within Pf1N1B4_2255 at 2571.459 kb on + strand, within Pf1N1B4_2255 at 2571.461 kb on + strand, within Pf1N1B4_2255 at 2571.485 kb on + strand, within Pf1N1B4_2255 at 2571.486 kb on - strand, within Pf1N1B4_2255 at 2571.486 kb on - strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.727 kb on + strand, within Pf1N1B4_2255 at 2571.728 kb on - strand, within Pf1N1B4_2255
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,568,741 + -0.4 2,568,742 - +0.4 2,568,742 - -0.2 2,568,742 - -1.4 2,568,754 - -0.2 2,568,754 - +0.0 2,568,754 - +0.1 2,568,765 + +0.0 2,568,765 + -0.0 2,568,766 - -1.2 2,568,766 - -1.2 2,568,766 - -1.2 2,568,840 + Pf1N1B4_2253 0.19 -0.9 2,568,840 + Pf1N1B4_2253 0.19 +0.8 2,568,840 + Pf1N1B4_2253 0.19 -0.4 2,568,840 + Pf1N1B4_2253 0.19 -0.2 2,568,840 + Pf1N1B4_2253 0.19 -2.2 2,568,840 + Pf1N1B4_2253 0.19 +1.4 2,568,840 + -0.6 2,568,840 + Pf1N1B4_2253 0.19 -0.9 2,568,840 + Pf1N1B4_2253 0.19 +0.7 2,568,840 + Pf1N1B4_2253 0.19 +0.4 2,568,840 + Pf1N1B4_2253 0.19 +1.0 2,568,840 + Pf1N1B4_2253 0.19 -0.3 2,568,840 + Pf1N1B4_2253 0.19 -0.0 2,568,840 + Pf1N1B4_2253 0.19 +0.8 2,568,841 - Pf1N1B4_2253 0.19 -0.9 2,568,841 - Pf1N1B4_2253 0.19 +0.7 2,568,841 - Pf1N1B4_2253 0.19 -0.2 2,568,841 - Pf1N1B4_2253 0.19 -0.2 2,568,841 - Pf1N1B4_2253 0.19 -0.9 2,568,841 - +1.4 2,568,841 - Pf1N1B4_2253 0.19 +1.1 2,568,841 - Pf1N1B4_2253 0.19 +2.4 2,568,841 - Pf1N1B4_2253 0.19 -0.8 2,569,035 + Pf1N1B4_2253 0.50 -0.8 2,569,035 + Pf1N1B4_2253 0.50 +1.8 2,569,035 + Pf1N1B4_2253 0.50 -0.2 2,569,036 - Pf1N1B4_2253 0.50 +0.0 2,569,233 + Pf1N1B4_2253 0.81 +1.4 2,569,381 + -0.2 2,569,381 + -2.7 2,569,381 + -2.8 2,569,382 - +0.7 2,569,382 - -1.9 2,569,382 - +0.7 2,569,429 + -1.9 2,569,464 + +0.1 2,569,464 + +2.4 2,569,464 + +1.0 2,569,464 + -0.2 2,569,464 + +0.9 2,569,464 + -0.0 2,569,464 + -0.3 2,569,464 + +0.8 2,569,464 + +1.0 2,569,464 + -0.4 2,569,464 + -1.4 2,569,464 + +0.1 2,569,464 + +1.2 2,569,465 - +2.0 2,569,465 - +1.4 2,569,465 - +1.3 2,569,465 - -1.9 2,569,465 - -0.8 2,569,465 - -0.7 2,569,465 - -1.4 2,569,465 - +1.4 2,569,465 - -0.4 2,569,465 - -0.6 2,569,465 - -0.2 2,569,465 - -2.0 2,569,465 - -1.7 2,569,465 - -0.6 2,569,465 - -0.6 2,569,494 - +0.0 2,569,524 + +0.4 2,569,524 + -0.0 2,569,545 + +0.7 2,569,580 + +1.0 2,569,595 + -0.2 2,569,596 - -0.4 2,569,604 + -0.9 2,569,604 + -0.5 2,569,604 + -0.6 2,569,604 + +3.0 2,569,605 - -0.1 2,569,605 - -1.4 2,569,605 - +0.4 2,569,605 - -0.5 2,569,654 - -1.6 2,569,676 + -0.6 2,569,676 + -0.8 2,569,676 + -1.0 2,569,676 + -0.6 2,569,676 + +0.5 2,569,677 - -0.7 2,569,677 - -1.4 2,569,691 + +1.4 2,569,691 + +0.2 2,569,691 + +1.2 2,569,691 + -0.6 2,569,691 + +0.8 2,569,692 - +0.6 2,569,692 - -1.2 2,569,692 - +3.0 2,569,692 - +1.0 2,569,692 - -1.2 2,569,692 - +2.0 2,569,772 + Pf1N1B4_2254 0.15 +0.4 2,569,772 + Pf1N1B4_2254 0.15 +0.8 2,569,773 - Pf1N1B4_2254 0.15 -0.2 2,569,773 - Pf1N1B4_2254 0.15 -0.7 2,569,786 + Pf1N1B4_2254 0.16 -0.2 2,569,786 + Pf1N1B4_2254 0.16 +0.8 2,569,787 - Pf1N1B4_2254 0.16 -2.5 2,569,942 - Pf1N1B4_2254 0.28 +0.1 2,569,979 + Pf1N1B4_2254 0.31 +0.6 2,569,979 + Pf1N1B4_2254 0.31 +0.6 2,569,979 + Pf1N1B4_2254 0.31 +0.8 2,569,979 + Pf1N1B4_2254 0.31 +0.4 2,569,979 + Pf1N1B4_2254 0.31 -0.1 2,569,979 + Pf1N1B4_2254 0.31 +1.8 2,569,979 + Pf1N1B4_2254 0.31 +0.4 2,569,979 + Pf1N1B4_2254 0.31 -1.8 2,569,979 + Pf1N1B4_2254 0.31 +0.2 2,569,979 + Pf1N1B4_2254 0.31 +1.8 2,569,980 - Pf1N1B4_2254 0.31 +0.5 2,569,980 - Pf1N1B4_2254 0.31 -0.6 2,569,980 - Pf1N1B4_2254 0.31 +0.7 2,569,980 - Pf1N1B4_2254 0.31 +0.2 2,569,980 - Pf1N1B4_2254 0.31 -0.2 2,569,980 - Pf1N1B4_2254 0.31 -0.4 2,570,003 + Pf1N1B4_2254 0.33 +1.4 2,570,130 + Pf1N1B4_2254 0.43 +1.1 2,570,220 - Pf1N1B4_2254 0.50 +0.3 2,570,397 - Pf1N1B4_2254 0.64 -0.4 2,570,397 - Pf1N1B4_2254 0.64 -1.8 2,570,397 - Pf1N1B4_2254 0.64 -0.0 2,570,420 + Pf1N1B4_2254 0.66 -1.3 2,570,421 - Pf1N1B4_2254 0.66 +0.5 2,570,507 + Pf1N1B4_2254 0.72 +1.0 2,570,508 - Pf1N1B4_2254 0.73 -0.4 2,570,508 - Pf1N1B4_2254 0.73 +2.2 2,570,632 + Pf1N1B4_2254 0.82 +1.1 2,570,633 - Pf1N1B4_2254 0.82 +1.1 2,570,761 - -0.2 2,570,773 + -1.8 2,570,773 + -0.4 2,570,773 + -1.7 2,570,774 - -0.2 2,570,774 - +1.4 2,570,774 - +0.2 2,570,805 - -0.2 2,570,805 - -0.2 2,570,859 + -0.6 2,570,882 + +1.8 2,570,883 - -1.2 2,570,883 - +0.6 2,570,982 - -1.5 2,571,201 - +0.7 2,571,217 + +0.8 2,571,217 + -0.2 2,571,218 - +0.1 2,571,218 - -0.2 2,571,315 + Pf1N1B4_2255 0.10 +0.4 2,571,315 + Pf1N1B4_2255 0.10 +0.9 2,571,315 + Pf1N1B4_2255 0.10 -0.2 2,571,315 + Pf1N1B4_2255 0.10 +1.5 2,571,315 + Pf1N1B4_2255 0.10 +0.5 2,571,315 + -0.6 2,571,315 + Pf1N1B4_2255 0.10 +1.2 2,571,315 + Pf1N1B4_2255 0.10 -1.0 2,571,315 + Pf1N1B4_2255 0.10 -1.9 2,571,316 - Pf1N1B4_2255 0.11 +1.9 2,571,316 - Pf1N1B4_2255 0.11 -2.0 2,571,316 - Pf1N1B4_2255 0.11 +0.2 2,571,316 - Pf1N1B4_2255 0.11 +0.1 2,571,316 - Pf1N1B4_2255 0.11 +0.8 2,571,316 - Pf1N1B4_2255 0.11 +0.2 2,571,319 + Pf1N1B4_2255 0.11 +1.0 2,571,320 - Pf1N1B4_2255 0.11 -1.0 2,571,379 + Pf1N1B4_2255 0.19 -1.4 2,571,380 - Pf1N1B4_2255 0.19 -1.2 2,571,380 - Pf1N1B4_2255 0.19 +0.0 2,571,380 - Pf1N1B4_2255 0.19 +1.3 2,571,380 - Pf1N1B4_2255 0.19 -0.6 2,571,440 + Pf1N1B4_2255 0.27 +1.1 2,571,440 + Pf1N1B4_2255 0.27 -1.2 2,571,440 + Pf1N1B4_2255 0.27 -1.4 2,571,441 - Pf1N1B4_2255 0.27 -0.7 2,571,441 - Pf1N1B4_2255 0.27 -0.6 2,571,441 - Pf1N1B4_2255 0.27 +0.2 2,571,441 - Pf1N1B4_2255 0.27 +1.4 2,571,441 - Pf1N1B4_2255 0.27 -0.6 2,571,443 + +2.4 2,571,443 + Pf1N1B4_2255 0.27 +2.0 2,571,445 + Pf1N1B4_2255 0.27 +1.4 2,571,445 + Pf1N1B4_2255 0.27 +0.2 2,571,445 + Pf1N1B4_2255 0.27 -1.6 2,571,445 + Pf1N1B4_2255 0.27 +1.6 2,571,445 + Pf1N1B4_2255 0.27 +0.4 2,571,445 + Pf1N1B4_2255 0.27 +0.3 2,571,445 + Pf1N1B4_2255 0.27 -0.2 2,571,445 + Pf1N1B4_2255 0.27 -0.2 2,571,446 - Pf1N1B4_2255 0.27 -1.5 2,571,446 - Pf1N1B4_2255 0.27 +2.4 2,571,446 - Pf1N1B4_2255 0.27 +1.1 2,571,446 - Pf1N1B4_2255 0.27 -1.6 2,571,446 - Pf1N1B4_2255 0.27 +0.3 2,571,446 - Pf1N1B4_2255 0.27 -1.2 2,571,446 - +0.8 2,571,446 - Pf1N1B4_2255 0.27 -0.9 2,571,446 - Pf1N1B4_2255 0.27 -0.6 2,571,446 - Pf1N1B4_2255 0.27 -0.9 2,571,459 + Pf1N1B4_2255 0.29 +1.4 2,571,459 + Pf1N1B4_2255 0.29 -0.1 2,571,461 + Pf1N1B4_2255 0.29 +0.8 2,571,485 + Pf1N1B4_2255 0.32 -0.2 2,571,486 - Pf1N1B4_2255 0.32 +1.2 2,571,486 - Pf1N1B4_2255 0.32 -0.3 2,571,727 + Pf1N1B4_2255 0.63 -2.3 2,571,727 + Pf1N1B4_2255 0.63 -0.2 2,571,727 + Pf1N1B4_2255 0.63 +0.2 2,571,727 + Pf1N1B4_2255 0.63 +0.4 2,571,727 + Pf1N1B4_2255 0.63 +1.7 2,571,728 - Pf1N1B4_2255 0.64 +0.8
Or see this region's nucleotide sequence