Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4270 and Psyr_4271 are separated by 104 nucleotides Psyr_4271 and Psyr_4272 are separated by 95 nucleotides Psyr_4272 and Psyr_4273 are separated by 27 nucleotides
Psyr_4270: Psyr_4270 - serine hydroxymethyltransferase, at 5,094,122 to 5,095,375
_4270
Psyr_4271: Psyr_4271 - Cobalamin synthesis protein/P47K:Cobalamin synthesis protein/P47K, at 5,095,480 to 5,096,457
_4271
Psyr_4272: Psyr_4272 - Protein of unknown function DUF466, at 5,096,553 to 5,096,750
_4272
Psyr_4273: Psyr_4273 - Carbon starvation protein CstA, at 5,096,778 to 5,098,853
_4273
Position (kb)
5095
5096
5097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5094.539 kb on + strand, within Psyr_4270 at 5094.539 kb on + strand, within Psyr_4270 at 5094.539 kb on + strand, within Psyr_4270 at 5094.540 kb on - strand, within Psyr_4270 at 5094.540 kb on - strand, within Psyr_4270 at 5094.540 kb on - strand, within Psyr_4270 at 5094.650 kb on + strand, within Psyr_4270 at 5094.707 kb on + strand, within Psyr_4270 at 5094.708 kb on - strand, within Psyr_4270 at 5094.977 kb on + strand, within Psyr_4270 at 5094.977 kb on + strand, within Psyr_4270 at 5094.977 kb on + strand, within Psyr_4270 at 5094.978 kb on - strand, within Psyr_4270 at 5095.364 kb on + strand at 5095.365 kb on - strand at 5095.448 kb on + strand at 5095.489 kb on + strand at 5095.489 kb on + strand at 5095.489 kb on + strand at 5095.489 kb on + strand at 5095.511 kb on - strand at 5095.602 kb on - strand, within Psyr_4271 at 5095.602 kb on - strand, within Psyr_4271 at 5095.602 kb on - strand, within Psyr_4271 at 5095.667 kb on + strand, within Psyr_4271 at 5095.668 kb on - strand, within Psyr_4271 at 5096.087 kb on + strand, within Psyr_4271 at 5096.087 kb on + strand, within Psyr_4271 at 5096.087 kb on + strand, within Psyr_4271 at 5096.087 kb on + strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.088 kb on - strand, within Psyr_4271 at 5096.199 kb on + strand, within Psyr_4271 at 5096.199 kb on + strand, within Psyr_4271 at 5096.200 kb on - strand, within Psyr_4271 at 5096.216 kb on + strand, within Psyr_4271 at 5096.216 kb on + strand, within Psyr_4271 at 5096.216 kb on + strand, within Psyr_4271 at 5096.216 kb on + strand, within Psyr_4271 at 5096.217 kb on - strand, within Psyr_4271 at 5096.284 kb on + strand, within Psyr_4271 at 5096.285 kb on - strand, within Psyr_4271 at 5096.457 kb on + strand at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.620 kb on + strand, within Psyr_4272 at 5096.621 kb on - strand, within Psyr_4272 at 5096.621 kb on - strand, within Psyr_4272 at 5096.621 kb on - strand, within Psyr_4272 at 5096.621 kb on - strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.668 kb on + strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.669 kb on - strand, within Psyr_4272 at 5096.677 kb on + strand, within Psyr_4272 at 5096.677 kb on + strand, within Psyr_4272 at 5096.677 kb on + strand, within Psyr_4272 at 5096.719 kb on + strand, within Psyr_4272 at 5096.720 kb on - strand, within Psyr_4272 at 5096.720 kb on - strand, within Psyr_4272 at 5096.720 kb on - strand, within Psyr_4272 at 5096.864 kb on + strand at 5096.864 kb on + strand at 5096.872 kb on + strand at 5096.873 kb on - strand at 5096.930 kb on - strand at 5097.010 kb on + strand, within Psyr_4273 at 5097.011 kb on - strand, within Psyr_4273 at 5097.011 kb on - strand, within Psyr_4273 at 5097.036 kb on + strand, within Psyr_4273 at 5097.127 kb on + strand, within Psyr_4273 at 5097.127 kb on + strand, within Psyr_4273 at 5097.128 kb on - strand, within Psyr_4273 at 5097.128 kb on - strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.224 kb on + strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.225 kb on - strand, within Psyr_4273 at 5097.260 kb on - strand, within Psyr_4273 at 5097.268 kb on + strand, within Psyr_4273 at 5097.446 kb on - strand, within Psyr_4273
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,094,539 + Psyr_4270 0.33 +3.5 5,094,539 + Psyr_4270 0.33 +1.7 5,094,539 + Psyr_4270 0.33 +3.7 5,094,540 - Psyr_4270 0.33 -1.0 5,094,540 - Psyr_4270 0.33 -0.7 5,094,540 - Psyr_4270 0.33 -0.8 5,094,650 + Psyr_4270 0.42 +4.6 5,094,707 + Psyr_4270 0.47 +0.6 5,094,708 - Psyr_4270 0.47 -0.5 5,094,977 + Psyr_4270 0.68 -0.6 5,094,977 + Psyr_4270 0.68 -1.2 5,094,977 + Psyr_4270 0.68 +1.2 5,094,978 - Psyr_4270 0.68 +4.9 5,095,364 + -1.4 5,095,365 - -1.1 5,095,448 + +2.7 5,095,489 + +4.2 5,095,489 + -1.6 5,095,489 + +1.7 5,095,489 + +0.7 5,095,511 - +1.2 5,095,602 - Psyr_4271 0.12 +0.7 5,095,602 - Psyr_4271 0.12 +1.8 5,095,602 - Psyr_4271 0.12 +1.2 5,095,667 + Psyr_4271 0.19 -3.3 5,095,668 - Psyr_4271 0.19 +2.5 5,096,087 + Psyr_4271 0.62 -1.6 5,096,087 + Psyr_4271 0.62 +1.0 5,096,087 + Psyr_4271 0.62 +3.2 5,096,087 + Psyr_4271 0.62 -2.0 5,096,088 - Psyr_4271 0.62 +1.0 5,096,088 - Psyr_4271 0.62 +1.2 5,096,199 + Psyr_4271 0.74 +3.3 5,096,199 + Psyr_4271 0.74 -0.9 5,096,200 - Psyr_4271 0.74 +2.0 5,096,216 + Psyr_4271 0.75 -0.4 5,096,216 + Psyr_4271 0.75 +0.8 5,096,216 + Psyr_4271 0.75 +1.7 5,096,216 + Psyr_4271 0.75 -0.7 5,096,217 - Psyr_4271 0.75 +0.2 5,096,284 + Psyr_4271 0.82 +3.6 5,096,285 - Psyr_4271 0.82 +3.2 5,096,457 + -1.0 5,096,620 + Psyr_4272 0.34 +3.9 5,096,620 + Psyr_4272 0.34 -1.8 5,096,620 + Psyr_4272 0.34 +1.2 5,096,620 + Psyr_4272 0.34 +2.6 5,096,620 + Psyr_4272 0.34 -0.5 5,096,621 - Psyr_4272 0.34 +0.6 5,096,621 - Psyr_4272 0.34 +0.2 5,096,621 - Psyr_4272 0.34 +0.7 5,096,621 - Psyr_4272 0.34 +0.4 5,096,668 + Psyr_4272 0.58 -2.1 5,096,668 + Psyr_4272 0.58 -1.7 5,096,668 + Psyr_4272 0.58 -1.2 5,096,668 + Psyr_4272 0.58 -0.0 5,096,668 + Psyr_4272 0.58 +0.3 5,096,668 + Psyr_4272 0.58 -0.9 5,096,668 + Psyr_4272 0.58 -0.7 5,096,668 + Psyr_4272 0.58 +2.5 5,096,668 + Psyr_4272 0.58 +2.5 5,096,669 - Psyr_4272 0.59 -1.8 5,096,669 - Psyr_4272 0.59 +3.5 5,096,669 - Psyr_4272 0.59 +0.1 5,096,669 - Psyr_4272 0.59 +1.7 5,096,669 - Psyr_4272 0.59 -0.5 5,096,669 - Psyr_4272 0.59 -0.3 5,096,677 + Psyr_4272 0.63 +3.0 5,096,677 + Psyr_4272 0.63 +0.6 5,096,677 + Psyr_4272 0.63 +2.1 5,096,719 + Psyr_4272 0.84 +0.8 5,096,720 - Psyr_4272 0.84 -0.3 5,096,720 - Psyr_4272 0.84 +3.2 5,096,720 - Psyr_4272 0.84 -0.3 5,096,864 + -0.9 5,096,864 + +3.5 5,096,872 + -0.5 5,096,873 - +3.6 5,096,930 - +2.5 5,097,010 + Psyr_4273 0.11 -0.7 5,097,011 - Psyr_4273 0.11 -2.2 5,097,011 - Psyr_4273 0.11 +2.2 5,097,036 + Psyr_4273 0.12 +0.2 5,097,127 + Psyr_4273 0.17 +3.5 5,097,127 + Psyr_4273 0.17 +0.6 5,097,128 - Psyr_4273 0.17 -2.0 5,097,128 - Psyr_4273 0.17 -0.0 5,097,224 + Psyr_4273 0.21 -0.6 5,097,224 + Psyr_4273 0.21 -1.5 5,097,224 + Psyr_4273 0.21 -1.5 5,097,225 - Psyr_4273 0.22 +1.2 5,097,225 - Psyr_4273 0.22 -0.1 5,097,225 - Psyr_4273 0.22 -2.8 5,097,225 - Psyr_4273 0.22 +2.2 5,097,260 - Psyr_4273 0.23 -0.6 5,097,268 + Psyr_4273 0.24 -1.2 5,097,446 - Psyr_4273 0.32 -1.7
Or see this region's nucleotide sequence