Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3769 and Psyr_3770 are separated by 302 nucleotides Psyr_3770 and Psyr_3771 are separated by 93 nucleotides Psyr_3771 and Psyr_3772 are separated by 55 nucleotides Psyr_3772 and Psyr_3773 are separated by 249 nucleotides
Psyr_3769: Psyr_3769 - TonB-dependent siderophore receptor, at 4,487,159 to 4,489,333
_3769
Psyr_3770: Psyr_3770 - Propeptide, PepSY amd peptidase M4, at 4,489,636 to 4,489,944
_3770
Psyr_3771: Psyr_3771 - conserved hypothetical protein, at 4,490,038 to 4,491,201
_3771
Psyr_3772: Psyr_3772 - conserved hypothetical protein, at 4,491,257 to 4,491,577
_3772
Psyr_3773: Psyr_3773 - GAF:ATP-binding region, ATPase-like:Histidine kinase A, N-terminal, at 4,491,827 to 4,493,011
_3773
Position (kb) 4490
4491
4492 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4489.181 kb on - strand at 4489.182 kb on + strand at 4489.402 kb on + strand at 4489.402 kb on + strand at 4489.402 kb on + strand at 4489.402 kb on + strand at 4489.403 kb on - strand at 4489.403 kb on - strand at 4489.403 kb on - strand at 4489.403 kb on - strand at 4489.431 kb on + strand at 4489.431 kb on + strand at 4489.431 kb on + strand at 4489.450 kb on - strand at 4489.478 kb on - strand at 4489.478 kb on - strand at 4489.478 kb on - strand at 4489.526 kb on + strand at 4489.527 kb on - strand at 4489.527 kb on - strand at 4489.527 kb on - strand at 4489.530 kb on + strand at 4489.541 kb on + strand at 4489.542 kb on - strand at 4489.546 kb on - strand at 4489.732 kb on + strand, within Psyr_3770 at 4489.732 kb on + strand, within Psyr_3770 at 4489.733 kb on - strand, within Psyr_3770 at 4489.733 kb on - strand, within Psyr_3770 at 4489.849 kb on + strand, within Psyr_3770 at 4489.849 kb on + strand, within Psyr_3770 at 4489.849 kb on + strand, within Psyr_3770 at 4489.850 kb on - strand, within Psyr_3770 at 4489.891 kb on + strand, within Psyr_3770 at 4489.891 kb on + strand, within Psyr_3770 at 4489.891 kb on + strand, within Psyr_3770 at 4489.892 kb on - strand, within Psyr_3770 at 4489.892 kb on - strand, within Psyr_3770 at 4489.922 kb on + strand at 4489.922 kb on + strand at 4489.922 kb on + strand at 4489.923 kb on - strand at 4489.923 kb on - strand at 4489.923 kb on - strand at 4489.999 kb on + strand at 4490.000 kb on - strand at 4490.000 kb on - strand at 4490.000 kb on - strand at 4490.053 kb on + strand at 4490.093 kb on + strand at 4490.093 kb on + strand at 4490.093 kb on + strand at 4490.093 kb on + strand at 4490.094 kb on - strand at 4490.094 kb on - strand at 4490.100 kb on - strand at 4490.128 kb on + strand at 4490.129 kb on - strand at 4490.129 kb on - strand at 4490.129 kb on - strand at 4490.230 kb on + strand, within Psyr_3771 at 4490.230 kb on + strand, within Psyr_3771 at 4490.230 kb on + strand, within Psyr_3771 at 4490.230 kb on + strand, within Psyr_3771 at 4490.230 kb on + strand, within Psyr_3771 at 4490.231 kb on - strand, within Psyr_3771 at 4490.231 kb on - strand, within Psyr_3771 at 4490.231 kb on - strand, within Psyr_3771 at 4490.231 kb on - strand, within Psyr_3771 at 4490.231 kb on - strand, within Psyr_3771 at 4490.267 kb on + strand, within Psyr_3771 at 4490.267 kb on + strand, within Psyr_3771 at 4490.267 kb on + strand, within Psyr_3771 at 4490.267 kb on + strand, within Psyr_3771 at 4490.267 kb on + strand, within Psyr_3771 at 4490.268 kb on - strand, within Psyr_3771 at 4490.268 kb on - strand, within Psyr_3771 at 4490.268 kb on - strand, within Psyr_3771 at 4490.268 kb on - strand, within Psyr_3771 at 4490.268 kb on - strand, within Psyr_3771 at 4490.372 kb on + strand, within Psyr_3771 at 4490.405 kb on + strand, within Psyr_3771 at 4490.428 kb on - strand, within Psyr_3771 at 4490.523 kb on - strand, within Psyr_3771 at 4490.523 kb on - strand at 4490.523 kb on - strand, within Psyr_3771 at 4490.530 kb on + strand, within Psyr_3771 at 4490.530 kb on + strand, within Psyr_3771 at 4490.530 kb on + strand, within Psyr_3771 at 4490.530 kb on + strand, within Psyr_3771 at 4490.530 kb on + strand, within Psyr_3771 at 4490.530 kb on + strand, within Psyr_3771 at 4490.531 kb on - strand, within Psyr_3771 at 4490.531 kb on - strand, within Psyr_3771 at 4490.859 kb on - strand, within Psyr_3771 at 4490.859 kb on - strand, within Psyr_3771 at 4490.868 kb on - strand, within Psyr_3771 at 4490.868 kb on - strand, within Psyr_3771 at 4490.868 kb on - strand, within Psyr_3771 at 4490.868 kb on - strand, within Psyr_3771 at 4490.868 kb on - strand, within Psyr_3771 at 4491.116 kb on + strand at 4491.168 kb on + strand at 4491.169 kb on - strand at 4491.169 kb on - strand at 4491.169 kb on - strand at 4491.196 kb on - strand at 4491.213 kb on + strand at 4491.214 kb on - strand at 4491.289 kb on + strand, within Psyr_3772 at 4491.289 kb on + strand, within Psyr_3772 at 4491.289 kb on + strand, within Psyr_3772 at 4491.289 kb on + strand, within Psyr_3772 at 4491.290 kb on - strand, within Psyr_3772 at 4491.290 kb on - strand, within Psyr_3772 at 4491.290 kb on - strand, within Psyr_3772 at 4491.317 kb on - strand, within Psyr_3772 at 4491.317 kb on - strand, within Psyr_3772 at 4491.454 kb on + strand, within Psyr_3772 at 4491.455 kb on - strand, within Psyr_3772 at 4491.455 kb on - strand, within Psyr_3772 at 4491.456 kb on + strand, within Psyr_3772 at 4491.457 kb on - strand, within Psyr_3772 at 4491.457 kb on - strand, within Psyr_3772 at 4491.559 kb on + strand at 4491.559 kb on + strand at 4491.581 kb on + strand at 4491.828 kb on + strand at 4491.828 kb on + strand at 4491.847 kb on + strand at 4491.847 kb on + strand at 4491.856 kb on + strand at 4491.857 kb on - strand at 4491.898 kb on + strand at 4491.898 kb on + strand at 4491.899 kb on - strand at 4491.933 kb on + strand at 4491.934 kb on - strand at 4491.934 kb on - strand at 4491.934 kb on - strand at 4491.934 kb on - strand at 4491.934 kb on - strand at 4491.934 kb on - strand at 4491.934 kb on - strand at 4492.187 kb on + strand, within Psyr_3773 at 4492.187 kb on + strand, within Psyr_3773 at 4492.187 kb on + strand, within Psyr_3773 at 4492.187 kb on + strand, within Psyr_3773 at 4492.188 kb on - strand, within Psyr_3773
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,489,181 - +2.0 4,489,182 + -0.5 4,489,402 + +3.0 4,489,402 + +0.8 4,489,402 + -2.3 4,489,402 + -0.3 4,489,403 - +1.5 4,489,403 - -3.1 4,489,403 - +2.3 4,489,403 - -1.8 4,489,431 + +3.8 4,489,431 + -0.5 4,489,431 + -1.5 4,489,450 - +0.1 4,489,478 - -1.8 4,489,478 - +0.5 4,489,478 - -0.6 4,489,526 + -1.8 4,489,527 - -2.0 4,489,527 - -0.8 4,489,527 - -0.6 4,489,530 + -1.3 4,489,541 + -1.9 4,489,542 - +1.5 4,489,546 - +0.2 4,489,732 + Psyr_3770 0.31 +2.5 4,489,732 + Psyr_3770 0.31 +0.2 4,489,733 - Psyr_3770 0.31 +0.9 4,489,733 - Psyr_3770 0.31 +2.3 4,489,849 + Psyr_3770 0.69 -0.8 4,489,849 + Psyr_3770 0.69 +0.5 4,489,849 + Psyr_3770 0.69 +3.8 4,489,850 - Psyr_3770 0.69 +3.3 4,489,891 + Psyr_3770 0.83 +3.3 4,489,891 + Psyr_3770 0.83 +0.6 4,489,891 + Psyr_3770 0.83 -0.3 4,489,892 - Psyr_3770 0.83 +3.4 4,489,892 - Psyr_3770 0.83 +2.5 4,489,922 + -2.5 4,489,922 + +4.4 4,489,922 + +4.0 4,489,923 - -2.5 4,489,923 - -0.8 4,489,923 - -2.6 4,489,999 + -2.3 4,490,000 - -0.7 4,490,000 - -0.3 4,490,000 - +2.8 4,490,053 + -0.0 4,490,093 + +3.1 4,490,093 + -1.0 4,490,093 + +3.1 4,490,093 + -1.9 4,490,094 - +0.4 4,490,094 - +4.0 4,490,100 - +2.8 4,490,128 + -0.8 4,490,129 - +0.3 4,490,129 - -0.1 4,490,129 - -2.5 4,490,230 + Psyr_3771 0.16 -1.9 4,490,230 + Psyr_3771 0.16 -1.2 4,490,230 + Psyr_3771 0.16 -0.7 4,490,230 + Psyr_3771 0.16 -1.8 4,490,230 + Psyr_3771 0.16 -1.6 4,490,231 - Psyr_3771 0.17 -1.3 4,490,231 - Psyr_3771 0.17 +2.8 4,490,231 - Psyr_3771 0.17 -2.7 4,490,231 - Psyr_3771 0.17 +0.0 4,490,231 - Psyr_3771 0.17 -0.5 4,490,267 + Psyr_3771 0.20 -2.2 4,490,267 + Psyr_3771 0.20 -0.7 4,490,267 + Psyr_3771 0.20 +0.4 4,490,267 + Psyr_3771 0.20 -2.4 4,490,267 + Psyr_3771 0.20 -0.0 4,490,268 - Psyr_3771 0.20 +3.3 4,490,268 - Psyr_3771 0.20 -1.7 4,490,268 - Psyr_3771 0.20 -3.3 4,490,268 - Psyr_3771 0.20 -1.4 4,490,268 - Psyr_3771 0.20 -0.4 4,490,372 + Psyr_3771 0.29 +0.9 4,490,405 + Psyr_3771 0.32 +0.6 4,490,428 - Psyr_3771 0.34 +0.8 4,490,523 - Psyr_3771 0.42 -0.9 4,490,523 - -0.0 4,490,523 - Psyr_3771 0.42 +2.0 4,490,530 + Psyr_3771 0.42 +1.5 4,490,530 + Psyr_3771 0.42 -0.8 4,490,530 + Psyr_3771 0.42 +3.7 4,490,530 + Psyr_3771 0.42 -2.8 4,490,530 + Psyr_3771 0.42 -0.9 4,490,530 + Psyr_3771 0.42 -0.9 4,490,531 - Psyr_3771 0.42 -0.5 4,490,531 - Psyr_3771 0.42 -1.9 4,490,859 - Psyr_3771 0.71 +0.2 4,490,859 - Psyr_3771 0.71 -1.6 4,490,868 - Psyr_3771 0.71 -0.7 4,490,868 - Psyr_3771 0.71 -0.2 4,490,868 - Psyr_3771 0.71 +2.0 4,490,868 - Psyr_3771 0.71 +3.9 4,490,868 - Psyr_3771 0.71 -1.2 4,491,116 + +3.1 4,491,168 + +3.5 4,491,169 - -1.4 4,491,169 - +4.0 4,491,169 - -1.3 4,491,196 - -1.7 4,491,213 + -2.5 4,491,214 - -1.0 4,491,289 + Psyr_3772 0.10 +3.5 4,491,289 + Psyr_3772 0.10 +1.5 4,491,289 + Psyr_3772 0.10 +3.1 4,491,289 + Psyr_3772 0.10 -2.2 4,491,290 - Psyr_3772 0.10 +1.0 4,491,290 - Psyr_3772 0.10 +0.1 4,491,290 - Psyr_3772 0.10 -1.7 4,491,317 - Psyr_3772 0.19 +0.6 4,491,317 - Psyr_3772 0.19 -2.7 4,491,454 + Psyr_3772 0.61 +3.3 4,491,455 - Psyr_3772 0.62 +0.3 4,491,455 - Psyr_3772 0.62 +3.5 4,491,456 + Psyr_3772 0.62 -2.3 4,491,457 - Psyr_3772 0.62 +3.0 4,491,457 - Psyr_3772 0.62 -3.1 4,491,559 + -2.2 4,491,559 + +4.1 4,491,581 + -2.3 4,491,828 + +2.3 4,491,828 + -1.1 4,491,847 + -3.6 4,491,847 + -0.0 4,491,856 + -2.2 4,491,857 - +1.5 4,491,898 + -1.9 4,491,898 + +2.8 4,491,899 - -3.2 4,491,933 + +3.8 4,491,934 - +0.5 4,491,934 - -0.8 4,491,934 - -1.2 4,491,934 - +0.1 4,491,934 - +2.5 4,491,934 - -0.3 4,491,934 - +4.1 4,492,187 + Psyr_3773 0.30 +2.0 4,492,187 + Psyr_3773 0.30 +0.4 4,492,187 + Psyr_3773 0.30 +3.3 4,492,187 + Psyr_3773 0.30 +0.9 4,492,188 - Psyr_3773 0.30 -3.9
Or see this region's nucleotide sequence