Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1366

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1364 and Psyr_1365 are separated by 13 nucleotidesPsyr_1365 and Psyr_1366 are separated by 7 nucleotidesPsyr_1366 and Psyr_1367 are separated by 104 nucleotides Psyr_1364: Psyr_1364 - cell division protein FtsB, at 1,548,383 to 1,548,739 _1364 Psyr_1365: Psyr_1365 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,548,753 to 1,549,463 _1365 Psyr_1366: Psyr_1366 - transcriptional regulator, LysR family, at 1,549,471 to 1,550,367 _1366 Psyr_1367: Psyr_1367 - Zinc-containing alcohol dehydrogenase superfamily, at 1,550,472 to 1,551,584 _1367 Position (kb) 1549 1550 1551Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1549.432 kb on + strandat 1549.462 kb on - strandat 1549.462 kb on - strandat 1549.509 kb on + strandat 1549.509 kb on + strandat 1549.515 kb on - strandat 1549.515 kb on - strandat 1549.515 kb on - strandat 1549.563 kb on - strand, within Psyr_1366at 1549.646 kb on + strand, within Psyr_1366at 1549.646 kb on + strand, within Psyr_1366at 1549.646 kb on + strand, within Psyr_1366at 1549.647 kb on - strand, within Psyr_1366at 1549.647 kb on - strand, within Psyr_1366at 1549.647 kb on - strand, within Psyr_1366at 1549.649 kb on + strand, within Psyr_1366at 1549.649 kb on + strand, within Psyr_1366at 1549.649 kb on + strand, within Psyr_1366at 1549.650 kb on - strand, within Psyr_1366at 1549.650 kb on - strand, within Psyr_1366at 1549.713 kb on + strand, within Psyr_1366at 1549.713 kb on + strand, within Psyr_1366at 1549.713 kb on + strand, within Psyr_1366at 1549.714 kb on - strand, within Psyr_1366at 1549.850 kb on + strand, within Psyr_1366at 1549.850 kb on + strand, within Psyr_1366at 1549.850 kb on + strand, within Psyr_1366at 1549.851 kb on - strand, within Psyr_1366at 1549.851 kb on - strand, within Psyr_1366at 1549.851 kb on - strand, within Psyr_1366at 1549.851 kb on - strand, within Psyr_1366at 1549.851 kb on - strand, within Psyr_1366at 1549.871 kb on + strand, within Psyr_1366at 1549.871 kb on + strand, within Psyr_1366at 1549.871 kb on + strand, within Psyr_1366at 1549.871 kb on + strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1549.872 kb on - strand, within Psyr_1366at 1550.009 kb on + strand, within Psyr_1366at 1550.010 kb on - strand, within Psyr_1366at 1550.363 kb on + strandat 1550.363 kb on + strandat 1550.363 kb on + strandat 1550.364 kb on - strandat 1550.364 kb on - strandat 1550.380 kb on + strandat 1550.430 kb on + strandat 1550.430 kb on + strandat 1550.430 kb on + strandat 1550.431 kb on - strandat 1550.434 kb on - strandat 1550.434 kb on - strandat 1550.575 kb on + strandat 1550.576 kb on - strandat 1550.576 kb on - strandat 1550.578 kb on + strandat 1550.579 kb on - strandat 1550.607 kb on + strand, within Psyr_1367at 1550.607 kb on + strand, within Psyr_1367at 1550.607 kb on + strand, within Psyr_1367at 1550.607 kb on + strand, within Psyr_1367at 1550.608 kb on - strand, within Psyr_1367at 1550.608 kb on - strand, within Psyr_1367at 1550.608 kb on - strand, within Psyr_1367at 1550.736 kb on + strand, within Psyr_1367at 1550.736 kb on + strand, within Psyr_1367at 1550.736 kb on + strand, within Psyr_1367at 1550.737 kb on - strand, within Psyr_1367at 1550.737 kb on - strand, within Psyr_1367at 1550.737 kb on - strand, within Psyr_1367at 1550.816 kb on + strand, within Psyr_1367at 1550.853 kb on + strand, within Psyr_1367at 1550.877 kb on + strand, within Psyr_1367at 1550.877 kb on + strand, within Psyr_1367at 1550.877 kb on + strand, within Psyr_1367at 1550.877 kb on + strand, within Psyr_1367at 1550.878 kb on - strand, within Psyr_1367at 1550.878 kb on - strand, within Psyr_1367at 1550.878 kb on - strand, within Psyr_1367at 1550.904 kb on + strand, within Psyr_1367at 1550.910 kb on + strand, within Psyr_1367at 1550.912 kb on - strand, within Psyr_1367

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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1,549,432 + +0.5
1,549,462 - +0.0
1,549,462 - +0.7
1,549,509 + +4.0
1,549,509 + -0.8
1,549,515 - -3.0
1,549,515 - +2.6
1,549,515 - +1.6
1,549,563 - Psyr_1366 0.10 +2.0
1,549,646 + Psyr_1366 0.20 +2.4
1,549,646 + Psyr_1366 0.20 -3.4
1,549,646 + Psyr_1366 0.20 +2.6
1,549,647 - Psyr_1366 0.20 -0.6
1,549,647 - Psyr_1366 0.20 +3.2
1,549,647 - Psyr_1366 0.20 -2.3
1,549,649 + Psyr_1366 0.20 -0.8
1,549,649 + Psyr_1366 0.20 +2.0
1,549,649 + Psyr_1366 0.20 -0.3
1,549,650 - Psyr_1366 0.20 +2.4
1,549,650 - Psyr_1366 0.20 -1.6
1,549,713 + Psyr_1366 0.27 +3.0
1,549,713 + Psyr_1366 0.27 +3.5
1,549,713 + Psyr_1366 0.27 -1.7
1,549,714 - Psyr_1366 0.27 -1.0
1,549,850 + Psyr_1366 0.42 -0.2
1,549,850 + Psyr_1366 0.42 -0.3
1,549,850 + Psyr_1366 0.42 +1.0
1,549,851 - Psyr_1366 0.42 -3.1
1,549,851 - Psyr_1366 0.42 +3.7
1,549,851 - Psyr_1366 0.42 +3.5
1,549,851 - Psyr_1366 0.42 +2.6
1,549,851 - Psyr_1366 0.42 -1.4
1,549,871 + Psyr_1366 0.45 +2.4
1,549,871 + Psyr_1366 0.45 +2.9
1,549,871 + Psyr_1366 0.45 +1.1
1,549,871 + Psyr_1366 0.45 +3.2
1,549,872 - Psyr_1366 0.45 +1.6
1,549,872 - Psyr_1366 0.45 +3.5
1,549,872 - Psyr_1366 0.45 +0.7
1,549,872 - Psyr_1366 0.45 +0.7
1,549,872 - Psyr_1366 0.45 -0.7
1,549,872 - Psyr_1366 0.45 -0.7
1,549,872 - Psyr_1366 0.45 +3.5
1,549,872 - Psyr_1366 0.45 -1.1
1,549,872 - Psyr_1366 0.45 -2.0
1,549,872 - Psyr_1366 0.45 -0.7
1,549,872 - Psyr_1366 0.45 -0.8
1,549,872 - Psyr_1366 0.45 -0.9
1,550,009 + Psyr_1366 0.60 +2.4
1,550,010 - Psyr_1366 0.60 +3.7
1,550,363 + +0.6
1,550,363 + -1.1
1,550,363 + +2.2
1,550,364 - -1.2
1,550,364 - -1.7
1,550,380 + +1.6
1,550,430 + +1.2
1,550,430 + -2.0
1,550,430 + +3.5
1,550,431 - -1.2
1,550,434 - -1.3
1,550,434 - +2.9
1,550,575 + +4.0
1,550,576 - +0.6
1,550,576 - +1.6
1,550,578 + -2.6
1,550,579 - +3.5
1,550,607 + Psyr_1367 0.12 +4.1
1,550,607 + Psyr_1367 0.12 +2.9
1,550,607 + Psyr_1367 0.12 -2.6
1,550,607 + Psyr_1367 0.12 -0.5
1,550,608 - Psyr_1367 0.12 -1.3
1,550,608 - Psyr_1367 0.12 +1.6
1,550,608 - Psyr_1367 0.12 -2.4
1,550,736 + Psyr_1367 0.24 -1.7
1,550,736 + Psyr_1367 0.24 +2.9
1,550,736 + Psyr_1367 0.24 +2.1
1,550,737 - Psyr_1367 0.24 -1.7
1,550,737 - Psyr_1367 0.24 +1.1
1,550,737 - Psyr_1367 0.24 +2.6
1,550,816 + Psyr_1367 0.31 -0.4
1,550,853 + Psyr_1367 0.34 -2.3
1,550,877 + Psyr_1367 0.36 -0.4
1,550,877 + Psyr_1367 0.36 +0.1
1,550,877 + Psyr_1367 0.36 -0.1
1,550,877 + Psyr_1367 0.36 +1.6
1,550,878 - Psyr_1367 0.36 +1.6
1,550,878 - Psyr_1367 0.36 -1.1
1,550,878 - Psyr_1367 0.36 -0.3
1,550,904 + Psyr_1367 0.39 +2.4
1,550,910 + Psyr_1367 0.39 -1.3
1,550,912 - Psyr_1367 0.40 +2.9

Or see this region's nucleotide sequence