Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1254 and Psyr_1255 are separated by 110 nucleotides Psyr_1255 and Psyr_1256 are separated by 69 nucleotides Psyr_1256 and Psyr_1257 are separated by 357 nucleotides
Psyr_1254: Psyr_1254 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase, at 1,410,450 to 1,411,241
_1254
Psyr_1255: Psyr_1255 - conserved hypothetical protein, at 1,411,352 to 1,411,789
_1255
Psyr_1256: Psyr_1256 - carboxyphosphonoenolpyruvate phosphonomutase, at 1,411,859 to 1,412,728
_1256
Psyr_1257: Psyr_1257 - 2-isopropylmalate synthase, at 1,413,086 to 1,414,756
_1257
Position (kb) 1411
1412
1413 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 1410.897 kb on + strand, within Psyr_1254 at 1410.898 kb on - strand, within Psyr_1254 at 1410.898 kb on - strand, within Psyr_1254 at 1410.954 kb on + strand, within Psyr_1254 at 1410.954 kb on + strand, within Psyr_1254 at 1410.955 kb on - strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.032 kb on + strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.033 kb on - strand, within Psyr_1254 at 1411.080 kb on + strand, within Psyr_1254 at 1411.080 kb on + strand, within Psyr_1254 at 1411.081 kb on - strand, within Psyr_1254 at 1411.229 kb on + strand at 1411.229 kb on + strand at 1411.229 kb on + strand at 1411.230 kb on - strand at 1411.230 kb on - strand at 1411.230 kb on - strand at 1411.233 kb on - strand at 1411.233 kb on - strand at 1411.233 kb on - strand at 1411.239 kb on + strand at 1411.273 kb on - strand at 1411.306 kb on + strand at 1411.306 kb on + strand at 1411.307 kb on - strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.315 kb on + strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.316 kb on - strand at 1411.335 kb on - strand at 1411.335 kb on - strand at 1411.335 kb on - strand at 1411.361 kb on - strand at 1411.536 kb on + strand, within Psyr_1255 at 1411.536 kb on + strand, within Psyr_1255 at 1411.536 kb on + strand, within Psyr_1255 at 1411.536 kb on + strand, within Psyr_1255 at 1411.537 kb on - strand, within Psyr_1255 at 1411.537 kb on - strand, within Psyr_1255 at 1411.537 kb on - strand, within Psyr_1255 at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.895 kb on + strand at 1411.896 kb on - strand at 1411.896 kb on - strand at 1411.896 kb on - strand at 1411.896 kb on - strand at 1411.899 kb on + strand at 1411.899 kb on + strand at 1411.899 kb on + strand at 1411.899 kb on + strand at 1411.900 kb on - strand at 1411.900 kb on - strand at 1411.900 kb on - strand at 1411.915 kb on + strand at 1411.915 kb on + strand at 1411.915 kb on + strand at 1411.916 kb on - strand at 1411.916 kb on - strand at 1411.917 kb on + strand at 1411.917 kb on + strand at 1411.917 kb on + strand at 1411.917 kb on + strand at 1411.917 kb on + strand at 1411.917 kb on + strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.918 kb on - strand at 1411.992 kb on + strand, within Psyr_1256 at 1411.992 kb on + strand, within Psyr_1256 at 1411.992 kb on + strand, within Psyr_1256 at 1411.992 kb on + strand, within Psyr_1256 at 1411.992 kb on + strand, within Psyr_1256 at 1411.993 kb on - strand, within Psyr_1256 at 1411.993 kb on - strand, within Psyr_1256 at 1411.993 kb on - strand, within Psyr_1256 at 1411.993 kb on - strand, within Psyr_1256 at 1412.016 kb on + strand, within Psyr_1256 at 1412.016 kb on + strand, within Psyr_1256 at 1412.017 kb on - strand, within Psyr_1256 at 1412.033 kb on + strand, within Psyr_1256 at 1412.033 kb on + strand, within Psyr_1256 at 1412.033 kb on + strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.034 kb on - strand, within Psyr_1256 at 1412.094 kb on + strand, within Psyr_1256 at 1412.095 kb on - strand, within Psyr_1256 at 1412.174 kb on - strand, within Psyr_1256 at 1412.199 kb on + strand, within Psyr_1256 at 1412.200 kb on - strand, within Psyr_1256 at 1412.374 kb on + strand, within Psyr_1256 at 1412.399 kb on - strand, within Psyr_1256 at 1412.597 kb on + strand, within Psyr_1256 at 1412.597 kb on + strand, within Psyr_1256 at 1412.597 kb on + strand, within Psyr_1256 at 1412.598 kb on - strand, within Psyr_1256 at 1412.598 kb on - strand, within Psyr_1256 at 1412.777 kb on + strand at 1412.778 kb on - strand at 1412.835 kb on + strand at 1412.835 kb on + strand at 1412.875 kb on - strand at 1412.875 kb on - strand at 1412.875 kb on - strand at 1412.875 kb on - strand at 1412.899 kb on + strand at 1412.899 kb on + strand at 1412.899 kb on + strand at 1412.899 kb on + strand at 1412.899 kb on + strand at 1412.900 kb on - strand at 1412.934 kb on - strand at 1412.934 kb on - strand at 1412.947 kb on + strand at 1412.947 kb on + strand at 1412.957 kb on + strand at 1412.957 kb on + strand at 1412.957 kb on + strand at 1412.957 kb on + strand at 1412.957 kb on + strand at 1412.957 kb on + strand at 1412.957 kb on + strand at 1412.958 kb on - strand at 1412.958 kb on - strand at 1412.958 kb on - strand at 1412.958 kb on - strand at 1412.958 kb on - strand at 1412.971 kb on + strand at 1413.222 kb on + strand at 1413.222 kb on + strand at 1413.222 kb on + strand at 1413.222 kb on + strand at 1413.222 kb on + strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.223 kb on - strand at 1413.270 kb on + strand, within Psyr_1257 at 1413.270 kb on + strand, within Psyr_1257 at 1413.270 kb on + strand, within Psyr_1257 at 1413.270 kb on + strand, within Psyr_1257 at 1413.271 kb on - strand, within Psyr_1257 at 1413.271 kb on - strand, within Psyr_1257 at 1413.381 kb on - strand, within Psyr_1257 at 1413.412 kb on + strand, within Psyr_1257 at 1413.412 kb on + strand, within Psyr_1257 at 1413.412 kb on + strand, within Psyr_1257 at 1413.412 kb on + strand, within Psyr_1257 at 1413.412 kb on + strand, within Psyr_1257 at 1413.412 kb on + strand, within Psyr_1257 at 1413.413 kb on - strand, within Psyr_1257 at 1413.413 kb on - strand, within Psyr_1257 at 1413.413 kb on - strand, within Psyr_1257 at 1413.413 kb on - strand, within Psyr_1257 at 1413.413 kb on - strand, within Psyr_1257 at 1413.429 kb on + strand, within Psyr_1257 at 1413.451 kb on - strand, within Psyr_1257 at 1413.451 kb on - strand, within Psyr_1257 at 1413.451 kb on - strand, within Psyr_1257 at 1413.571 kb on - strand, within Psyr_1257 at 1413.588 kb on + strand, within Psyr_1257 at 1413.588 kb on + strand, within Psyr_1257 at 1413.639 kb on + strand, within Psyr_1257 at 1413.639 kb on + strand, within Psyr_1257 at 1413.640 kb on - strand, within Psyr_1257 at 1413.675 kb on + strand, within Psyr_1257 at 1413.676 kb on - strand, within Psyr_1257
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,410,897 + Psyr_1254 0.56 +0.9 1,410,898 - Psyr_1254 0.57 -0.0 1,410,898 - Psyr_1254 0.57 -0.0 1,410,954 + Psyr_1254 0.64 +0.3 1,410,954 + Psyr_1254 0.64 -0.7 1,410,955 - Psyr_1254 0.64 +0.3 1,411,032 + Psyr_1254 0.73 +2.3 1,411,032 + Psyr_1254 0.73 -0.0 1,411,032 + Psyr_1254 0.73 +2.8 1,411,032 + Psyr_1254 0.73 -0.0 1,411,032 + Psyr_1254 0.73 +2.6 1,411,032 + Psyr_1254 0.73 -1.3 1,411,032 + Psyr_1254 0.73 +4.4 1,411,032 + Psyr_1254 0.73 +0.6 1,411,032 + Psyr_1254 0.73 -0.4 1,411,033 - Psyr_1254 0.74 -0.0 1,411,033 - Psyr_1254 0.74 +0.4 1,411,033 - Psyr_1254 0.74 +4.4 1,411,033 - Psyr_1254 0.74 +0.2 1,411,033 - Psyr_1254 0.74 +4.0 1,411,033 - Psyr_1254 0.74 -1.1 1,411,033 - Psyr_1254 0.74 +3.0 1,411,033 - Psyr_1254 0.74 -0.8 1,411,033 - Psyr_1254 0.74 +0.5 1,411,033 - Psyr_1254 0.74 -0.0 1,411,033 - Psyr_1254 0.74 -1.4 1,411,033 - Psyr_1254 0.74 -1.3 1,411,033 - Psyr_1254 0.74 +2.8 1,411,033 - Psyr_1254 0.74 -2.5 1,411,080 + Psyr_1254 0.80 +3.7 1,411,080 + Psyr_1254 0.80 +0.2 1,411,081 - Psyr_1254 0.80 -1.3 1,411,229 + +2.8 1,411,229 + -0.6 1,411,229 + +0.8 1,411,230 - -0.6 1,411,230 - -4.9 1,411,230 - -2.7 1,411,233 - +0.3 1,411,233 - +1.6 1,411,233 - +1.6 1,411,239 + -0.0 1,411,273 - -1.2 1,411,306 + -0.8 1,411,306 + -2.2 1,411,307 - +0.3 1,411,315 + +2.3 1,411,315 + +0.6 1,411,315 + -3.3 1,411,315 + -0.7 1,411,315 + +3.2 1,411,315 + +4.8 1,411,315 + +0.8 1,411,315 + +3.0 1,411,316 - +2.0 1,411,316 - -1.4 1,411,316 - +1.6 1,411,316 - +1.6 1,411,316 - -1.7 1,411,316 - +2.0 1,411,316 - +1.6 1,411,316 - +2.8 1,411,316 - -0.0 1,411,316 - +2.8 1,411,316 - +2.0 1,411,316 - +2.8 1,411,316 - -1.1 1,411,316 - +1.0 1,411,316 - +2.0 1,411,316 - +2.0 1,411,335 - +1.6 1,411,335 - -1.3 1,411,335 - +3.2 1,411,361 - +0.2 1,411,536 + Psyr_1255 0.42 -0.9 1,411,536 + Psyr_1255 0.42 -1.4 1,411,536 + Psyr_1255 0.42 -0.7 1,411,536 + Psyr_1255 0.42 -0.6 1,411,537 - Psyr_1255 0.42 -0.6 1,411,537 - Psyr_1255 0.42 +3.2 1,411,537 - Psyr_1255 0.42 -0.0 1,411,895 + -2.7 1,411,895 + +3.6 1,411,895 + -2.6 1,411,895 + -1.7 1,411,895 + -1.9 1,411,895 + -1.9 1,411,895 + -1.3 1,411,895 + +2.6 1,411,895 + +3.2 1,411,895 + -0.9 1,411,896 - -0.6 1,411,896 - -0.1 1,411,896 - -4.1 1,411,896 - -1.1 1,411,899 + -1.4 1,411,899 + +3.7 1,411,899 + -1.8 1,411,899 + -0.8 1,411,900 - -0.9 1,411,900 - +3.9 1,411,900 - -1.2 1,411,915 + -1.6 1,411,915 + -0.2 1,411,915 + +3.2 1,411,916 - -3.9 1,411,916 - -1.4 1,411,917 + +1.0 1,411,917 + -1.3 1,411,917 + +3.3 1,411,917 + +1.0 1,411,917 + -0.0 1,411,917 + +3.2 1,411,918 - +3.4 1,411,918 - -2.0 1,411,918 - +0.6 1,411,918 - +2.0 1,411,918 - -2.0 1,411,918 - +1.0 1,411,918 - -1.2 1,411,918 - -1.4 1,411,918 - +0.3 1,411,918 - -0.1 1,411,992 + Psyr_1256 0.15 -0.0 1,411,992 + Psyr_1256 0.15 +3.0 1,411,992 + Psyr_1256 0.15 -1.8 1,411,992 + Psyr_1256 0.15 -4.2 1,411,992 + Psyr_1256 0.15 +1.7 1,411,993 - Psyr_1256 0.15 -2.2 1,411,993 - Psyr_1256 0.15 -0.0 1,411,993 - Psyr_1256 0.15 +3.4 1,411,993 - Psyr_1256 0.15 -2.4 1,412,016 + Psyr_1256 0.18 -1.1 1,412,016 + Psyr_1256 0.18 -0.0 1,412,017 - Psyr_1256 0.18 +1.0 1,412,033 + Psyr_1256 0.20 +2.3 1,412,033 + Psyr_1256 0.20 -0.5 1,412,033 + Psyr_1256 0.20 +0.1 1,412,034 - Psyr_1256 0.20 -1.3 1,412,034 - Psyr_1256 0.20 -0.7 1,412,034 - Psyr_1256 0.20 +0.2 1,412,034 - Psyr_1256 0.20 -2.1 1,412,034 - Psyr_1256 0.20 -1.6 1,412,034 - Psyr_1256 0.20 -1.6 1,412,034 - Psyr_1256 0.20 +1.0 1,412,034 - Psyr_1256 0.20 +2.3 1,412,034 - Psyr_1256 0.20 -0.7 1,412,034 - Psyr_1256 0.20 -0.5 1,412,094 + Psyr_1256 0.27 +1.6 1,412,095 - Psyr_1256 0.27 -4.7 1,412,174 - Psyr_1256 0.36 -1.0 1,412,199 + Psyr_1256 0.39 +1.6 1,412,200 - Psyr_1256 0.39 +4.6 1,412,374 + Psyr_1256 0.59 -1.0 1,412,399 - Psyr_1256 0.62 -0.8 1,412,597 + Psyr_1256 0.85 -3.7 1,412,597 + Psyr_1256 0.85 -2.3 1,412,597 + Psyr_1256 0.85 +2.6 1,412,598 - Psyr_1256 0.85 +3.6 1,412,598 - Psyr_1256 0.85 -1.5 1,412,777 + -0.9 1,412,778 - -1.9 1,412,835 + +1.0 1,412,835 + +1.9 1,412,875 - -3.7 1,412,875 - +1.0 1,412,875 - -2.2 1,412,875 - -2.6 1,412,899 + -1.7 1,412,899 + -0.0 1,412,899 + +0.1 1,412,899 + +0.1 1,412,899 + +0.7 1,412,900 - -1.4 1,412,934 - +1.3 1,412,934 - +1.0 1,412,947 + +2.8 1,412,947 + -0.6 1,412,957 + -0.6 1,412,957 + +1.1 1,412,957 + +3.8 1,412,957 + +2.0 1,412,957 + +2.0 1,412,957 + -0.2 1,412,957 + -1.1 1,412,958 - +1.6 1,412,958 - -0.0 1,412,958 - -0.8 1,412,958 - +2.3 1,412,958 - -3.5 1,412,971 + +2.3 1,413,222 + -2.6 1,413,222 + -1.8 1,413,222 + -1.8 1,413,222 + -1.9 1,413,222 + +2.3 1,413,223 - -2.2 1,413,223 - -1.4 1,413,223 - -0.0 1,413,223 - +2.8 1,413,223 - -1.7 1,413,223 - +2.6 1,413,223 - -2.0 1,413,223 - -0.0 1,413,270 + Psyr_1257 0.11 -4.3 1,413,270 + Psyr_1257 0.11 -1.6 1,413,270 + Psyr_1257 0.11 +2.3 1,413,270 + Psyr_1257 0.11 +1.6 1,413,271 - Psyr_1257 0.11 +0.7 1,413,271 - Psyr_1257 0.11 -0.1 1,413,381 - Psyr_1257 0.18 -0.2 1,413,412 + Psyr_1257 0.20 +0.2 1,413,412 + Psyr_1257 0.20 -3.6 1,413,412 + Psyr_1257 0.20 +2.0 1,413,412 + Psyr_1257 0.20 -0.3 1,413,412 + Psyr_1257 0.20 -2.0 1,413,412 + Psyr_1257 0.20 +3.0 1,413,413 - Psyr_1257 0.20 +2.3 1,413,413 - Psyr_1257 0.20 +1.0 1,413,413 - Psyr_1257 0.20 +3.9 1,413,413 - Psyr_1257 0.20 -1.8 1,413,413 - Psyr_1257 0.20 -0.8 1,413,429 + Psyr_1257 0.21 +2.8 1,413,451 - Psyr_1257 0.22 +3.0 1,413,451 - Psyr_1257 0.22 -0.7 1,413,451 - Psyr_1257 0.22 -3.0 1,413,571 - Psyr_1257 0.29 +0.3 1,413,588 + Psyr_1257 0.30 -2.8 1,413,588 + Psyr_1257 0.30 -3.7 1,413,639 + Psyr_1257 0.33 -1.3 1,413,639 + Psyr_1257 0.33 +1.0 1,413,640 - Psyr_1257 0.33 +2.8 1,413,675 + Psyr_1257 0.35 +3.6 1,413,676 - Psyr_1257 0.35 +2.6
Or see this region's nucleotide sequence