Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0814

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0812 and Psyr_0813 are separated by 37 nucleotidesPsyr_0813 and Psyr_0814 are separated by 209 nucleotidesPsyr_0814 and Psyr_0815 are separated by 248 nucleotides Psyr_0812: Psyr_0812 - stress protein, at 917,011 to 917,586 _0812 Psyr_0813: Psyr_0813 - stress protein, at 917,624 to 918,202 _0813 Psyr_0814: Psyr_0814 - Protein of unknown function DUF124, at 918,412 to 919,287 _0814 Psyr_0815: Psyr_0815 - nicotinate-nucleotide pyrophosphorylase (carboxylating), at 919,536 to 920,384 _0815 Position (kb) 918 919 920Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 917.492 kb on - strand, within Psyr_0812at 917.549 kb on + strandat 917.550 kb on - strandat 917.631 kb on + strandat 917.631 kb on + strandat 917.632 kb on - strandat 917.632 kb on - strandat 917.641 kb on + strandat 917.697 kb on + strand, within Psyr_0813at 917.698 kb on - strand, within Psyr_0813at 917.810 kb on + strand, within Psyr_0813at 917.810 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.005 kb on + strand, within Psyr_0813at 918.006 kb on - strand, within Psyr_0813at 918.006 kb on - strand, within Psyr_0813at 918.006 kb on - strand, within Psyr_0813at 918.006 kb on - strand, within Psyr_0813at 918.006 kb on - strand, within Psyr_0813at 918.006 kb on - strand, within Psyr_0813at 918.104 kb on + strand, within Psyr_0813at 918.104 kb on + strand, within Psyr_0813at 918.253 kb on + strandat 918.393 kb on + strandat 918.393 kb on + strandat 918.393 kb on + strandat 918.393 kb on + strandat 918.394 kb on - strandat 918.394 kb on - strandat 918.394 kb on - strandat 918.401 kb on + strandat 918.401 kb on + strandat 918.401 kb on + strandat 918.402 kb on - strandat 918.402 kb on - strandat 918.402 kb on - strandat 918.431 kb on - strandat 918.587 kb on + strand, within Psyr_0814at 918.588 kb on - strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.052 kb on + strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.053 kb on - strand, within Psyr_0814at 919.130 kb on + strand, within Psyr_0814at 919.131 kb on - strand, within Psyr_0814at 919.173 kb on - strand, within Psyr_0814at 919.200 kb on + strandat 919.356 kb on - strandat 919.356 kb on - strandat 919.356 kb on - strandat 919.471 kb on + strandat 919.472 kb on - strandat 919.495 kb on + strandat 919.509 kb on + strandat 919.509 kb on + strandat 919.510 kb on - strandat 919.604 kb on - strandat 919.604 kb on - strandat 919.604 kb on - strandat 919.604 kb on - strandat 919.894 kb on + strand, within Psyr_0815at 919.939 kb on + strand, within Psyr_0815at 919.939 kb on + strand, within Psyr_0815at 919.939 kb on + strand, within Psyr_0815at 919.940 kb on - strand, within Psyr_0815at 919.940 kb on - strand, within Psyr_0815at 919.940 kb on - strand, within Psyr_0815at 919.940 kb on - strand, within Psyr_0815at 920.023 kb on + strand, within Psyr_0815at 920.023 kb on + strand, within Psyr_0815at 920.023 kb on + strand, within Psyr_0815at 920.023 kb on + strand, within Psyr_0815at 920.023 kb on + strand, within Psyr_0815at 920.024 kb on - strand, within Psyr_0815at 920.024 kb on - strand, within Psyr_0815at 920.238 kb on - strand, within Psyr_0815

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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917,492 - Psyr_0812 0.84 -0.7
917,549 + +1.2
917,550 - -2.7
917,631 + +4.6
917,631 + +2.7
917,632 - -0.5
917,632 - -1.8
917,641 + +0.2
917,697 + Psyr_0813 0.13 +0.6
917,698 - Psyr_0813 0.13 +0.2
917,810 + Psyr_0813 0.32 -0.4
917,810 + Psyr_0813 0.32 -1.9
918,005 + Psyr_0813 0.66 +2.7
918,005 + Psyr_0813 0.66 +0.4
918,005 + Psyr_0813 0.66 -0.9
918,005 + Psyr_0813 0.66 -0.6
918,005 + Psyr_0813 0.66 -1.3
918,005 + Psyr_0813 0.66 +1.1
918,005 + Psyr_0813 0.66 -1.4
918,005 + Psyr_0813 0.66 -1.0
918,006 - Psyr_0813 0.66 -0.6
918,006 - Psyr_0813 0.66 +2.5
918,006 - Psyr_0813 0.66 +4.3
918,006 - Psyr_0813 0.66 -1.3
918,006 - Psyr_0813 0.66 +2.5
918,006 - Psyr_0813 0.66 +0.8
918,104 + Psyr_0813 0.83 -1.3
918,104 + Psyr_0813 0.83 -0.9
918,253 + +2.7
918,393 + -0.6
918,393 + -1.5
918,393 + -2.0
918,393 + -2.1
918,394 - -2.3
918,394 - +0.6
918,394 - -3.3
918,401 + +1.7
918,401 + +0.1
918,401 + +2.5
918,402 - +0.5
918,402 - +0.5
918,402 - +0.1
918,431 - +4.5
918,587 + Psyr_0814 0.20 +2.9
918,588 - Psyr_0814 0.20 +0.6
919,052 + Psyr_0814 0.73 -1.8
919,052 + Psyr_0814 0.73 +4.5
919,052 + Psyr_0814 0.73 +1.1
919,052 + Psyr_0814 0.73 +0.9
919,052 + Psyr_0814 0.73 +0.1
919,052 + Psyr_0814 0.73 -1.9
919,052 + Psyr_0814 0.73 -1.6
919,052 + Psyr_0814 0.73 +4.0
919,052 + Psyr_0814 0.73 +2.1
919,052 + Psyr_0814 0.73 +4.4
919,053 - Psyr_0814 0.73 +3.8
919,053 - Psyr_0814 0.73 -1.1
919,053 - Psyr_0814 0.73 -0.4
919,053 - Psyr_0814 0.73 +0.1
919,053 - Psyr_0814 0.73 +1.7
919,053 - Psyr_0814 0.73 -1.6
919,053 - Psyr_0814 0.73 +2.5
919,053 - Psyr_0814 0.73 -1.4
919,130 + Psyr_0814 0.82 +3.3
919,131 - Psyr_0814 0.82 +3.3
919,173 - Psyr_0814 0.87 -2.7
919,200 + -0.2
919,356 - +1.0
919,356 - -1.0
919,356 - +1.1
919,471 + -0.6
919,472 - -1.1
919,495 + +2.7
919,509 + -0.1
919,509 + -0.5
919,510 - -0.9
919,604 - -1.8
919,604 - -0.4
919,604 - -1.7
919,604 - +0.1
919,894 + Psyr_0815 0.42 -1.5
919,939 + Psyr_0815 0.47 -0.6
919,939 + Psyr_0815 0.47 -1.6
919,939 + Psyr_0815 0.47 -0.4
919,940 - Psyr_0815 0.48 -1.3
919,940 - Psyr_0815 0.48 -0.3
919,940 - Psyr_0815 0.48 -2.5
919,940 - Psyr_0815 0.48 +1.2
920,023 + Psyr_0815 0.57 +1.1
920,023 + Psyr_0815 0.57 -0.8
920,023 + Psyr_0815 0.57 -0.7
920,023 + Psyr_0815 0.57 -0.9
920,023 + Psyr_0815 0.57 +0.9
920,024 - Psyr_0815 0.57 +3.5
920,024 - Psyr_0815 0.57 +1.7
920,238 - Psyr_0815 0.83 +3.7

Or see this region's nucleotide sequence