Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0435 and Psyr_0436 overlap by 4 nucleotides Psyr_0436 and Psyr_0437 are separated by 9 nucleotides Psyr_0437 and Psyr_0438 overlap by 26 nucleotides Psyr_0438 and Psyr_0439 overlap by 25 nucleotides
Psyr_0435: Psyr_0435 - membrane protein, putative, at 474,481 to 475,026
_0435
Psyr_0436: Psyr_0436 - Phosphopantetheine-binding protein, at 475,023 to 475,277
_0436
Psyr_0437: Psyr_0437 - acyl carrier protein, putative, at 475,287 to 475,547
_0437
Psyr_0438: Psyr_0438 - Phospholipid/glycerol acyltransferase, at 475,522 to 476,334
_0438
Psyr_0439: Psyr_0439 - conserved hypothetical protein, at 476,310 to 477,029
_0439
Position (kb)
475
476
477 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 474.661 kb on - strand, within Psyr_0435 at 474.669 kb on - strand, within Psyr_0435 at 475.049 kb on - strand, within Psyr_0436 at 475.049 kb on - strand, within Psyr_0436 at 475.059 kb on + strand, within Psyr_0436 at 475.060 kb on - strand, within Psyr_0436 at 475.460 kb on + strand, within Psyr_0437 at 475.461 kb on - strand, within Psyr_0437 at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.523 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.571 kb on + strand at 475.572 kb on - strand at 475.572 kb on - strand at 475.584 kb on - strand at 475.584 kb on - strand at 475.645 kb on + strand, within Psyr_0438 at 475.645 kb on + strand, within Psyr_0438 at 475.645 kb on + strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.646 kb on - strand, within Psyr_0438 at 475.919 kb on + strand, within Psyr_0438 at 475.920 kb on - strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.093 kb on + strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.094 kb on - strand, within Psyr_0438 at 476.288 kb on + strand at 476.288 kb on + strand at 476.289 kb on - strand at 476.289 kb on - strand at 476.291 kb on + strand at 476.291 kb on + strand at 476.527 kb on + strand, within Psyr_0439 at 476.527 kb on + strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.566 kb on - strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.614 kb on + strand, within Psyr_0439 at 476.615 kb on - strand, within Psyr_0439 at 476.615 kb on - strand, within Psyr_0439 at 477.066 kb on + strand at 477.067 kb on - strand at 477.141 kb on + strand at 477.141 kb on + strand at 477.141 kb on + strand at 477.142 kb on - strand at 477.145 kb on - strand at 477.160 kb on + strand at 477.160 kb on + strand at 477.161 kb on - strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.181 kb on + strand at 477.182 kb on - strand at 477.182 kb on - strand at 477.256 kb on + strand at 477.257 kb on - strand at 477.267 kb on + strand at 477.268 kb on - strand at 477.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 474,661 - Psyr_0435 0.33 -1.9 474,669 - Psyr_0435 0.34 +2.7 475,049 - Psyr_0436 0.10 +5.1 475,049 - Psyr_0436 0.10 +0.1 475,059 + Psyr_0436 0.14 +1.7 475,060 - Psyr_0436 0.15 +3.8 475,460 + Psyr_0437 0.66 -2.1 475,461 - Psyr_0437 0.67 -0.8 475,523 + -1.1 475,523 + -1.7 475,523 + -1.3 475,523 + +4.1 475,523 + +1.1 475,523 + +3.3 475,571 + +1.7 475,571 + +2.5 475,571 + -1.1 475,571 + -1.4 475,571 + -1.2 475,571 + +5.4 475,571 + +2.5 475,571 + +0.3 475,572 - +2.1 475,572 - -0.6 475,584 - -1.4 475,584 - -1.4 475,645 + Psyr_0438 0.15 +0.4 475,645 + Psyr_0438 0.15 +2.1 475,645 + Psyr_0438 0.15 +2.9 475,646 - Psyr_0438 0.15 -1.0 475,646 - Psyr_0438 0.15 +0.1 475,646 - Psyr_0438 0.15 +1.7 475,646 - Psyr_0438 0.15 +2.5 475,646 - Psyr_0438 0.15 +3.1 475,646 - Psyr_0438 0.15 -2.2 475,919 + Psyr_0438 0.49 +3.9 475,920 - Psyr_0438 0.49 -1.9 476,093 + Psyr_0438 0.70 -1.9 476,093 + Psyr_0438 0.70 -2.0 476,093 + Psyr_0438 0.70 +0.3 476,093 + Psyr_0438 0.70 -1.7 476,093 + Psyr_0438 0.70 +1.7 476,093 + Psyr_0438 0.70 -0.6 476,093 + Psyr_0438 0.70 -0.6 476,093 + Psyr_0438 0.70 -2.9 476,093 + Psyr_0438 0.70 -1.1 476,093 + Psyr_0438 0.70 -1.4 476,094 - Psyr_0438 0.70 -2.9 476,094 - Psyr_0438 0.70 +0.2 476,094 - Psyr_0438 0.70 -0.9 476,094 - Psyr_0438 0.70 -0.3 476,288 + -0.8 476,288 + +4.4 476,289 - +0.1 476,289 - +3.3 476,291 + +0.1 476,291 + -2.3 476,527 + Psyr_0439 0.30 -0.5 476,527 + Psyr_0439 0.30 +3.7 476,566 - Psyr_0439 0.36 -0.3 476,566 - Psyr_0439 0.36 -1.4 476,566 - Psyr_0439 0.36 +0.1 476,614 + Psyr_0439 0.42 -2.2 476,614 + Psyr_0439 0.42 +0.1 476,614 + Psyr_0439 0.42 -0.2 476,614 + Psyr_0439 0.42 +2.1 476,614 + Psyr_0439 0.42 -1.1 476,615 - Psyr_0439 0.42 -0.4 476,615 - Psyr_0439 0.42 +4.3 477,066 + +0.5 477,067 - +4.7 477,141 + +0.6 477,141 + +0.1 477,141 + -0.7 477,142 - -0.8 477,145 - -1.2 477,160 + -0.3 477,160 + +0.1 477,161 - +1.8 477,181 + +2.1 477,181 + -2.3 477,181 + -1.9 477,181 + +3.5 477,182 - +1.1 477,182 - +2.9 477,256 + -0.3 477,257 - +1.1 477,267 + -1.9 477,268 - -0.6 477,268 - +2.5
Or see this region's nucleotide sequence