Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0344 and Psyr_0345 overlap by 4 nucleotides Psyr_0345 and Psyr_0346 overlap by 4 nucleotides
Psyr_0344: Psyr_0344 - Secretion protein HlyD, at 366,781 to 367,899
_0344
Psyr_0345: Psyr_0345 - Secretion protein HlyD, at 367,896 to 368,981
_0345
Psyr_0346: Psyr_0346 - Acriflavin resistance protein, at 368,978 to 372,079
_0346
Position (kb)
367
368
369 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 367.177 kb on + strand, within Psyr_0344 at 367.177 kb on + strand, within Psyr_0344 at 367.178 kb on - strand, within Psyr_0344 at 367.179 kb on + strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.180 kb on - strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.195 kb on + strand, within Psyr_0344 at 367.196 kb on - strand, within Psyr_0344 at 367.417 kb on + strand, within Psyr_0344 at 367.418 kb on - strand, within Psyr_0344 at 367.418 kb on - strand, within Psyr_0344 at 367.515 kb on - strand, within Psyr_0344 at 367.542 kb on + strand, within Psyr_0344 at 367.700 kb on + strand, within Psyr_0344 at 367.832 kb on + strand at 367.832 kb on + strand at 367.832 kb on + strand at 367.833 kb on - strand at 367.833 kb on - strand at 367.948 kb on + strand at 367.949 kb on - strand at 367.949 kb on - strand at 368.128 kb on + strand, within Psyr_0345 at 368.129 kb on - strand, within Psyr_0345 at 368.534 kb on + strand, within Psyr_0345 at 368.566 kb on + strand, within Psyr_0345 at 368.566 kb on + strand, within Psyr_0345 at 368.567 kb on - strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.654 kb on + strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.655 kb on - strand, within Psyr_0345 at 368.723 kb on - strand, within Psyr_0345 at 368.850 kb on - strand, within Psyr_0345 at 369.035 kb on + strand at 369.036 kb on - strand at 369.074 kb on + strand at 369.224 kb on + strand at 369.225 kb on - strand at 369.225 kb on - strand at 369.225 kb on - strand at 369.225 kb on - strand at 369.236 kb on + strand at 369.236 kb on + strand at 369.236 kb on + strand at 369.236 kb on + strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.237 kb on - strand at 369.239 kb on - strand at 369.269 kb on + strand at 369.269 kb on + strand at 369.269 kb on + strand at 369.270 kb on - strand at 369.270 kb on - strand at 369.315 kb on - strand, within Psyr_0346 at 369.405 kb on - strand, within Psyr_0346 at 369.416 kb on + strand, within Psyr_0346 at 369.416 kb on + strand, within Psyr_0346 at 369.416 kb on + strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.417 kb on - strand, within Psyr_0346 at 369.461 kb on + strand, within Psyr_0346 at 369.461 kb on + strand, within Psyr_0346 at 369.461 kb on + strand, within Psyr_0346 at 369.462 kb on - strand, within Psyr_0346 at 369.462 kb on - strand, within Psyr_0346 at 369.462 kb on - strand, within Psyr_0346 at 369.545 kb on + strand, within Psyr_0346 at 369.545 kb on + strand, within Psyr_0346 at 369.546 kb on - strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.898 kb on + strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.899 kb on - strand, within Psyr_0346 at 369.907 kb on + strand, within Psyr_0346 at 369.908 kb on - strand, within Psyr_0346
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 367,177 + Psyr_0344 0.35 -2.0 367,177 + Psyr_0344 0.35 +1.1 367,178 - Psyr_0344 0.35 -1.6 367,179 + Psyr_0344 0.36 -1.3 367,180 - Psyr_0344 0.36 +0.3 367,180 - Psyr_0344 0.36 +1.7 367,180 - Psyr_0344 0.36 +0.0 367,180 - Psyr_0344 0.36 +3.3 367,195 + Psyr_0344 0.37 +1.7 367,195 + Psyr_0344 0.37 -0.9 367,195 + Psyr_0344 0.37 -0.7 367,195 + Psyr_0344 0.37 +0.4 367,196 - Psyr_0344 0.37 -0.9 367,417 + Psyr_0344 0.57 -3.3 367,418 - Psyr_0344 0.57 -1.8 367,418 - Psyr_0344 0.57 -1.2 367,515 - Psyr_0344 0.66 +0.6 367,542 + Psyr_0344 0.68 +3.7 367,700 + Psyr_0344 0.82 -1.1 367,832 + +3.7 367,832 + +0.1 367,832 + -2.4 367,833 - -0.9 367,833 - -0.9 367,948 + -1.0 367,949 - -4.0 367,949 - -3.9 368,128 + Psyr_0345 0.21 +3.7 368,129 - Psyr_0345 0.21 -1.1 368,534 + Psyr_0345 0.59 +2.7 368,566 + Psyr_0345 0.62 +2.5 368,566 + Psyr_0345 0.62 +1.1 368,567 - Psyr_0345 0.62 +2.5 368,654 + Psyr_0345 0.70 -1.0 368,654 + Psyr_0345 0.70 -1.4 368,654 + Psyr_0345 0.70 -2.0 368,655 - Psyr_0345 0.70 -1.1 368,655 - Psyr_0345 0.70 -1.3 368,655 - Psyr_0345 0.70 +0.6 368,723 - Psyr_0345 0.76 +2.5 368,850 - Psyr_0345 0.88 +1.6 369,035 + +1.0 369,036 - +3.1 369,074 + -2.4 369,224 + +2.1 369,225 - +0.3 369,225 - +0.9 369,225 - +0.1 369,225 - -3.3 369,236 + -0.1 369,236 + -0.9 369,236 + -0.1 369,236 + -2.4 369,237 - -0.1 369,237 - +0.8 369,237 - +3.5 369,237 - -1.0 369,237 - +0.9 369,237 - +0.5 369,237 - -0.5 369,239 - -0.9 369,269 + +4.0 369,269 + +0.1 369,269 + +1.7 369,270 - +3.1 369,270 - -1.5 369,315 - Psyr_0346 0.11 -1.9 369,405 - Psyr_0346 0.14 +2.5 369,416 + Psyr_0346 0.14 -0.9 369,416 + Psyr_0346 0.14 +3.8 369,416 + Psyr_0346 0.14 -1.6 369,417 - Psyr_0346 0.14 -0.1 369,417 - Psyr_0346 0.14 -0.2 369,417 - Psyr_0346 0.14 -0.2 369,417 - Psyr_0346 0.14 -1.7 369,461 + Psyr_0346 0.16 +0.1 369,461 + Psyr_0346 0.16 +0.9 369,461 + Psyr_0346 0.16 +0.1 369,462 - Psyr_0346 0.16 -2.1 369,462 - Psyr_0346 0.16 +0.1 369,462 - Psyr_0346 0.16 -0.9 369,545 + Psyr_0346 0.18 -0.1 369,545 + Psyr_0346 0.18 -0.9 369,546 - Psyr_0346 0.18 -1.0 369,898 + Psyr_0346 0.30 +3.3 369,898 + Psyr_0346 0.30 -0.5 369,898 + Psyr_0346 0.30 +1.3 369,898 + Psyr_0346 0.30 -2.1 369,899 - Psyr_0346 0.30 -0.8 369,899 - Psyr_0346 0.30 +0.3 369,899 - Psyr_0346 0.30 +0.3 369,899 - Psyr_0346 0.30 +1.0 369,899 - Psyr_0346 0.30 +3.7 369,907 + Psyr_0346 0.30 +4.7 369,908 - Psyr_0346 0.30 +2.9
Or see this region's nucleotide sequence