Experiment: Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0497 and Dsui_0498 are separated by 16 nucleotides Dsui_0498 and Dsui_0499 are separated by 27 nucleotides Dsui_0499 and Dsui_0500 are separated by 31 nucleotides
Dsui_0497: Dsui_0497 - TIGR00255 family protein, at 499,496 to 500,362
_0497
Dsui_0498: Dsui_0498 - guanylate kinase, at 500,379 to 500,987
_0498
Dsui_0499: Dsui_0499 - DNA-directed RNA polymerase, omega subunit, at 501,015 to 501,221
_0499
Dsui_0500: Dsui_0500 - (p)ppGpp synthetase, RelA/SpoT family, at 501,253 to 503,484
_0500
Position (kb) 501
502 Strain fitness (log2 ratio) -1
0
1 at 500.026 kb on + strand, within Dsui_0497 at 500.049 kb on + strand, within Dsui_0497 at 500.077 kb on + strand, within Dsui_0497 at 500.147 kb on + strand, within Dsui_0497 at 500.164 kb on + strand, within Dsui_0497 at 500.287 kb on - strand at 500.293 kb on + strand at 501.006 kb on + strand at 501.035 kb on + strand at 501.040 kb on + strand, within Dsui_0499 at 501.134 kb on - strand, within Dsui_0499 at 501.215 kb on + strand at 501.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Anaerobic growth in rich media with perchlorate electron acceptor, 1000 uM Stressor (H2S) spiked in when cells reach OD 0.5 remove 500,026 + Dsui_0497 0.61 -0.2 500,049 + Dsui_0497 0.64 -0.3 500,077 + Dsui_0497 0.67 +0.2 500,147 + Dsui_0497 0.75 +0.3 500,164 + Dsui_0497 0.77 +1.0 500,287 - -0.5 500,293 + -0.1 501,006 + -0.5 501,035 + -0.3 501,040 + Dsui_0499 0.12 -0.5 501,134 - Dsui_0499 0.57 -0.5 501,215 + +0.7 501,230 - -0.7
Or see this region's nucleotide sequence