Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1389 and SO1390 are separated by 60 nucleotides SO1390 and SO1391 are separated by 221 nucleotides SO1391 and SO1392 are separated by 80 nucleotides SO1392 and SO1393 are separated by 274 nucleotides
SO1389: SO1389 - ROK family protein (NCBI ptt file), at 1,446,733 to 1,447,527
SO1389
SO1390: SO1390 - peptidyl-prolyl cis-trans isomerase, FKBP-type (NCBI ptt file), at 1,447,588 to 1,448,058
SO1390
SO1391: SO1391 - hypothetical Short chain fatty acid transporter (NCBI ptt file), at 1,448,280 to 1,449,611
SO1391
SO1392: SO1392 - conserved hypothetical protein (NCBI ptt file), at 1,449,692 to 1,450,171
SO1392
SO1393: SO1393 - transcriptional regulator, TetR family (NCBI ptt file), at 1,450,446 to 1,451,063
SO1393
Position (kb)
1448
1449
1450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1447.297 kb on + strand, within SO1389 at 1447.343 kb on - strand, within SO1389 at 1447.373 kb on + strand, within SO1389 at 1447.445 kb on - strand, within SO1389 at 1447.605 kb on + strand at 1447.626 kb on - strand at 1447.744 kb on - strand, within SO1390 at 1447.763 kb on + strand, within SO1390 at 1447.825 kb on - strand, within SO1390 at 1447.841 kb on - strand, within SO1390 at 1447.918 kb on - strand, within SO1390 at 1448.186 kb on + strand at 1448.228 kb on - strand at 1448.275 kb on + strand at 1448.280 kb on + strand at 1448.283 kb on - strand at 1448.283 kb on - strand at 1448.283 kb on - strand at 1448.283 kb on - strand at 1448.283 kb on - strand at 1448.288 kb on - strand at 1448.406 kb on + strand at 1448.481 kb on - strand, within SO1391 at 1448.655 kb on + strand, within SO1391 at 1448.655 kb on + strand, within SO1391 at 1448.663 kb on - strand, within SO1391 at 1448.741 kb on - strand, within SO1391 at 1448.759 kb on - strand, within SO1391 at 1448.777 kb on - strand, within SO1391 at 1448.795 kb on - strand, within SO1391 at 1448.923 kb on + strand, within SO1391 at 1448.923 kb on + strand, within SO1391 at 1448.948 kb on - strand, within SO1391 at 1448.952 kb on + strand, within SO1391 at 1448.957 kb on + strand, within SO1391 at 1448.965 kb on - strand, within SO1391 at 1449.039 kb on - strand, within SO1391 at 1449.051 kb on - strand, within SO1391 at 1449.051 kb on - strand, within SO1391 at 1449.056 kb on + strand, within SO1391 at 1449.086 kb on - strand, within SO1391 at 1449.099 kb on - strand, within SO1391 at 1449.160 kb on + strand, within SO1391 at 1449.168 kb on - strand, within SO1391 at 1449.291 kb on + strand, within SO1391 at 1449.299 kb on - strand, within SO1391 at 1449.505 kb on - strand at 1449.531 kb on - strand at 1449.553 kb on + strand at 1449.589 kb on + strand at 1449.589 kb on - strand at 1449.798 kb on + strand, within SO1392 at 1449.856 kb on + strand, within SO1392 at 1449.901 kb on + strand, within SO1392 at 1449.901 kb on + strand, within SO1392 at 1449.901 kb on + strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1449.909 kb on - strand, within SO1392 at 1450.116 kb on + strand, within SO1392 at 1450.124 kb on - strand at 1450.124 kb on - strand at 1450.236 kb on - strand at 1450.289 kb on - strand at 1450.463 kb on - strand at 1450.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 1,447,297 + SO1389 0.71 -2.5 1,447,343 - SO1389 0.77 -1.9 1,447,373 + SO1389 0.81 -2.0 1,447,445 - SO1389 0.90 +3.2 1,447,605 + -1.5 1,447,626 - -1.5 1,447,744 - SO1390 0.33 +0.2 1,447,763 + SO1390 0.37 -0.8 1,447,825 - SO1390 0.50 +0.7 1,447,841 - SO1390 0.54 -0.6 1,447,918 - SO1390 0.70 -1.2 1,448,186 + +0.9 1,448,228 - -1.0 1,448,275 + -0.9 1,448,280 + -1.3 1,448,283 - +0.4 1,448,283 - +0.4 1,448,283 - -0.6 1,448,283 - +0.1 1,448,283 - -0.3 1,448,288 - +1.1 1,448,406 + +0.4 1,448,481 - SO1391 0.15 +0.0 1,448,655 + SO1391 0.28 +1.5 1,448,655 + SO1391 0.28 +0.7 1,448,663 - SO1391 0.29 -1.2 1,448,741 - SO1391 0.35 -0.2 1,448,759 - SO1391 0.36 -0.6 1,448,777 - SO1391 0.37 +0.1 1,448,795 - SO1391 0.39 +0.2 1,448,923 + SO1391 0.48 -0.7 1,448,923 + SO1391 0.48 -0.9 1,448,948 - SO1391 0.50 +0.3 1,448,952 + SO1391 0.50 +0.4 1,448,957 + SO1391 0.51 +1.1 1,448,965 - SO1391 0.51 +0.7 1,449,039 - SO1391 0.57 +1.2 1,449,051 - SO1391 0.58 +1.4 1,449,051 - SO1391 0.58 +1.0 1,449,056 + SO1391 0.58 -0.2 1,449,086 - SO1391 0.61 -0.3 1,449,099 - SO1391 0.61 +1.4 1,449,160 + SO1391 0.66 -0.4 1,449,168 - SO1391 0.67 -0.9 1,449,291 + SO1391 0.76 -0.7 1,449,299 - SO1391 0.77 +0.3 1,449,505 - -0.1 1,449,531 - +0.9 1,449,553 + -0.3 1,449,589 + +0.5 1,449,589 - -2.0 1,449,798 + SO1392 0.22 +0.0 1,449,856 + SO1392 0.34 +1.0 1,449,901 + SO1392 0.44 +0.8 1,449,901 + SO1392 0.44 +0.0 1,449,901 + SO1392 0.44 +0.6 1,449,909 - SO1392 0.45 +0.0 1,449,909 - SO1392 0.45 +0.0 1,449,909 - SO1392 0.45 +0.8 1,449,909 - SO1392 0.45 -0.6 1,449,909 - SO1392 0.45 -0.0 1,449,909 - SO1392 0.45 -3.2 1,449,909 - SO1392 0.45 +3.5 1,449,909 - SO1392 0.45 +0.9 1,450,116 + SO1392 0.88 -0.5 1,450,124 - -1.0 1,450,124 - +0.8 1,450,236 - -0.5 1,450,289 - -0.3 1,450,463 - +0.1 1,450,471 - +0.7
Or see this region's nucleotide sequence