Experiment: 36hr PHYTO11 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02405 and BPHYT_RS02410 are separated by 8 nucleotides BPHYT_RS02410 and BPHYT_RS02415 are separated by 320 nucleotides BPHYT_RS02415 and BPHYT_RS02420 are separated by 13 nucleotides BPHYT_RS02420 and BPHYT_RS02425 overlap by 29 nucleotides
BPHYT_RS02405: BPHYT_RS02405 - ferredoxin, at 548,841 to 549,164
_RS02405
BPHYT_RS02410: BPHYT_RS02410 - alpha/beta hydrolase, at 549,173 to 549,817
_RS02410
BPHYT_RS02415: BPHYT_RS02415 - cytochrome C550, at 550,138 to 551,367
_RS02415
BPHYT_RS02420: BPHYT_RS02420 - cytochrome C550, at 551,381 to 552,346
_RS02420
BPHYT_RS02425: BPHYT_RS02425 - hypothetical protein, at 552,318 to 552,587
_RS02425
Position (kb) 550
551
552 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 549.162 kb on + strand at 549.162 kb on + strand at 549.163 kb on - strand at 549.163 kb on - strand at 549.163 kb on - strand at 549.163 kb on - strand at 549.180 kb on + strand at 549.180 kb on + strand at 549.180 kb on + strand at 549.180 kb on + strand at 549.180 kb on + strand at 549.181 kb on - strand at 549.181 kb on - strand at 549.181 kb on - strand at 549.375 kb on - strand, within BPHYT_RS02410 at 549.375 kb on - strand, within BPHYT_RS02410 at 549.375 kb on - strand at 549.383 kb on + strand, within BPHYT_RS02410 at 549.383 kb on + strand, within BPHYT_RS02410 at 549.816 kb on - strand at 549.851 kb on + strand at 549.868 kb on + strand at 549.869 kb on - strand at 549.891 kb on + strand at 549.891 kb on + strand at 549.911 kb on + strand at 549.911 kb on + strand at 549.912 kb on - strand at 550.138 kb on - strand at 550.138 kb on - strand at 550.397 kb on - strand, within BPHYT_RS02415 at 550.540 kb on + strand, within BPHYT_RS02415 at 550.596 kb on - strand, within BPHYT_RS02415 at 550.687 kb on - strand, within BPHYT_RS02415 at 550.706 kb on - strand, within BPHYT_RS02415 at 550.768 kb on + strand, within BPHYT_RS02415 at 550.769 kb on - strand, within BPHYT_RS02415 at 550.798 kb on + strand, within BPHYT_RS02415 at 550.798 kb on + strand, within BPHYT_RS02415 at 550.798 kb on + strand, within BPHYT_RS02415 at 551.054 kb on - strand, within BPHYT_RS02415 at 551.054 kb on - strand, within BPHYT_RS02415 at 551.137 kb on + strand, within BPHYT_RS02415 at 551.137 kb on + strand, within BPHYT_RS02415 at 551.137 kb on + strand, within BPHYT_RS02415 at 551.138 kb on - strand, within BPHYT_RS02415 at 551.365 kb on + strand at 551.365 kb on + strand at 551.366 kb on - strand at 551.366 kb on - strand at 551.420 kb on + strand at 551.421 kb on - strand at 551.502 kb on - strand, within BPHYT_RS02420 at 551.520 kb on - strand, within BPHYT_RS02420 at 551.520 kb on - strand, within BPHYT_RS02420 at 551.714 kb on + strand, within BPHYT_RS02420 at 551.716 kb on + strand, within BPHYT_RS02420 at 551.716 kb on + strand, within BPHYT_RS02420 at 551.781 kb on + strand, within BPHYT_RS02420 at 551.782 kb on - strand, within BPHYT_RS02420 at 551.782 kb on - strand, within BPHYT_RS02420 at 551.924 kb on + strand, within BPHYT_RS02420 at 551.925 kb on - strand, within BPHYT_RS02420 at 551.925 kb on - strand, within BPHYT_RS02420 at 552.257 kb on + strand at 552.266 kb on + strand at 552.266 kb on + strand at 552.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO11 MOI 1 remove 549,162 + +1.1 549,162 + -0.3 549,163 - -0.1 549,163 - -0.5 549,163 - +0.2 549,163 - -0.0 549,180 + +0.4 549,180 + -0.3 549,180 + -0.9 549,180 + -0.1 549,180 + +0.0 549,181 - -1.3 549,181 - -0.4 549,181 - -1.0 549,375 - BPHYT_RS02410 0.31 +1.2 549,375 - BPHYT_RS02410 0.31 -0.2 549,375 - +2.4 549,383 + BPHYT_RS02410 0.33 -1.5 549,383 + BPHYT_RS02410 0.33 +0.7 549,816 - -0.2 549,851 + +0.0 549,868 + +0.6 549,869 - +1.9 549,891 + -0.2 549,891 + -0.9 549,911 + -0.6 549,911 + -0.1 549,912 - -0.1 550,138 - +1.4 550,138 - -2.1 550,397 - BPHYT_RS02415 0.21 -1.1 550,540 + BPHYT_RS02415 0.33 -1.1 550,596 - BPHYT_RS02415 0.37 +0.2 550,687 - BPHYT_RS02415 0.45 -1.8 550,706 - BPHYT_RS02415 0.46 +0.4 550,768 + BPHYT_RS02415 0.51 -1.3 550,769 - BPHYT_RS02415 0.51 +4.0 550,798 + BPHYT_RS02415 0.54 -0.4 550,798 + BPHYT_RS02415 0.54 +1.4 550,798 + BPHYT_RS02415 0.54 +1.2 551,054 - BPHYT_RS02415 0.74 -0.9 551,054 - BPHYT_RS02415 0.74 +0.4 551,137 + BPHYT_RS02415 0.81 -0.7 551,137 + BPHYT_RS02415 0.81 +0.4 551,137 + BPHYT_RS02415 0.81 -0.1 551,138 - BPHYT_RS02415 0.81 +1.4 551,365 + -0.0 551,365 + -0.4 551,366 - +1.0 551,366 - -0.3 551,420 + -0.4 551,421 - -2.1 551,502 - BPHYT_RS02420 0.13 +0.2 551,520 - BPHYT_RS02420 0.14 +1.4 551,520 - BPHYT_RS02420 0.14 -1.1 551,714 + BPHYT_RS02420 0.34 -0.8 551,716 + BPHYT_RS02420 0.35 +0.1 551,716 + BPHYT_RS02420 0.35 -0.3 551,781 + BPHYT_RS02420 0.41 -1.7 551,782 - BPHYT_RS02420 0.42 +0.1 551,782 - BPHYT_RS02420 0.42 -0.0 551,924 + BPHYT_RS02420 0.56 +0.5 551,925 - BPHYT_RS02420 0.56 +0.1 551,925 - BPHYT_RS02420 0.56 -1.3 552,257 + +2.4 552,266 + +0.2 552,266 + -0.4 552,313 - -0.5
Or see this region's nucleotide sequence