Experiment: 36hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05545 and BPHYT_RS05550 are separated by 99 nucleotides BPHYT_RS05550 and BPHYT_RS05555 overlap by 4 nucleotides BPHYT_RS05555 and BPHYT_RS05560 are separated by 362 nucleotides
BPHYT_RS05545: BPHYT_RS05545 - nitrate ABC transporter ATP-binding protein, at 1,274,077 to 1,275,423
_RS05545
BPHYT_RS05550: BPHYT_RS05550 - hypothetical protein, at 1,275,523 to 1,276,092
_RS05550
BPHYT_RS05555: BPHYT_RS05555 - hypothetical protein, at 1,276,089 to 1,276,667
_RS05555
BPHYT_RS05560: BPHYT_RS05560 - Fis family transcriptional regulator, at 1,277,030 to 1,278,088
_RS05560
Position (kb) 1275
1276
1277 Strain fitness (log2 ratio) -1
0
1 at 1275.376 kb on + strand at 1275.833 kb on + strand, within BPHYT_RS05550 at 1275.833 kb on + strand, within BPHYT_RS05550 at 1276.386 kb on - strand, within BPHYT_RS05555 at 1276.750 kb on - strand at 1276.750 kb on - strand at 1276.781 kb on + strand at 1276.781 kb on + strand at 1276.781 kb on + strand at 1276.788 kb on - strand at 1276.937 kb on - strand at 1276.969 kb on - strand at 1277.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO7 MOI 1 remove 1,275,376 + -0.3 1,275,833 + BPHYT_RS05550 0.54 -1.2 1,275,833 + BPHYT_RS05550 0.54 -1.2 1,276,386 - BPHYT_RS05555 0.51 +0.5 1,276,750 - -0.0 1,276,750 - +1.6 1,276,781 + +0.5 1,276,781 + +0.1 1,276,781 + +1.1 1,276,788 - +0.2 1,276,937 - -0.7 1,276,969 - -1.0 1,277,085 + +1.7
Or see this region's nucleotide sequence