Experiment: 24hr PHYTO7 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24580 and BPHYT_RS24585 are separated by 222 nucleotides BPHYT_RS24585 and BPHYT_RS24590 are separated by 116 nucleotides BPHYT_RS24590 and BPHYT_RS24595 are separated by 483 nucleotides
BPHYT_RS24580: BPHYT_RS24580 - hypothetical protein, at 1,084,272 to 1,084,601
_RS24580
BPHYT_RS24585: BPHYT_RS24585 - NUDIX hydrolase, at 1,084,824 to 1,085,411
_RS24585
BPHYT_RS24590: BPHYT_RS24590 - DeoR faimly transcriptional regulator, at 1,085,528 to 1,086,289
_RS24590
BPHYT_RS24595: BPHYT_RS24595 - aldehyde oxidase, at 1,086,773 to 1,089,601
_RS24595
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1084.737 kb on - strand at 1084.742 kb on + strand at 1084.999 kb on + strand, within BPHYT_RS24585 at 1086.098 kb on + strand, within BPHYT_RS24590 at 1086.236 kb on - strand at 1086.333 kb on + strand at 1086.333 kb on + strand at 1086.675 kb on + strand at 1087.070 kb on + strand, within BPHYT_RS24595 at 1087.070 kb on + strand, within BPHYT_RS24595 at 1087.070 kb on + strand, within BPHYT_RS24595 at 1087.071 kb on - strand, within BPHYT_RS24595 at 1087.172 kb on + strand, within BPHYT_RS24595 at 1087.214 kb on + strand, within BPHYT_RS24595
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO7 MOI 1 remove 1,084,737 - +0.2 1,084,742 + -1.1 1,084,999 + BPHYT_RS24585 0.30 +0.8 1,086,098 + BPHYT_RS24590 0.75 -2.9 1,086,236 - -2.0 1,086,333 + -0.1 1,086,333 + +0.3 1,086,675 + +2.4 1,087,070 + BPHYT_RS24595 0.10 +1.8 1,087,070 + BPHYT_RS24595 0.10 -0.3 1,087,070 + BPHYT_RS24595 0.10 +0.7 1,087,071 - BPHYT_RS24595 0.11 +0.6 1,087,172 + BPHYT_RS24595 0.14 -0.7 1,087,214 + BPHYT_RS24595 0.16 +0.0
Or see this region's nucleotide sequence