Experiment: 36hr PHYTO10 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33355 and BPHYT_RS33360 are separated by 24 nucleotides BPHYT_RS33360 and BPHYT_RS33365 are separated by 105 nucleotides BPHYT_RS33365 and BPHYT_RS33370 are separated by 80 nucleotides
BPHYT_RS33355: BPHYT_RS33355 - oxalyl-CoA decarboxylase, at 3,066,064 to 3,067,806
_RS33355
BPHYT_RS33360: BPHYT_RS33360 - formyl-CoA transferase, at 3,067,831 to 3,069,081
_RS33360
BPHYT_RS33365: BPHYT_RS33365 - PAS sensor protein, at 3,069,187 to 3,069,618
_RS33365
BPHYT_RS33370: BPHYT_RS33370 - LysR family transcriptional regulator, at 3,069,699 to 3,070,625
_RS33370
Position (kb) 3067
3068
3069
3070 Strain fitness (log2 ratio) -2
-1
0
1 at 3067.538 kb on + strand, within BPHYT_RS33355 at 3067.539 kb on - strand, within BPHYT_RS33355 at 3068.228 kb on - strand, within BPHYT_RS33360 at 3068.519 kb on - strand, within BPHYT_RS33360 at 3068.630 kb on - strand, within BPHYT_RS33360 at 3069.089 kb on + strand at 3069.327 kb on + strand, within BPHYT_RS33365 at 3069.450 kb on + strand, within BPHYT_RS33365 at 3069.892 kb on - strand, within BPHYT_RS33370
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO10 MOI 10 remove 3,067,538 + BPHYT_RS33355 0.85 -0.8 3,067,539 - BPHYT_RS33355 0.85 -0.8 3,068,228 - BPHYT_RS33360 0.32 +0.2 3,068,519 - BPHYT_RS33360 0.55 -0.8 3,068,630 - BPHYT_RS33360 0.64 -2.0 3,069,089 + -1.0 3,069,327 + BPHYT_RS33365 0.32 -1.0 3,069,450 + BPHYT_RS33365 0.61 -2.0 3,069,892 - BPHYT_RS33370 0.21 +0.3
Or see this region's nucleotide sequence