Experiment: 36hr PHYTO4 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00935 and BPHYT_RS00940 are separated by 205 nucleotides BPHYT_RS00940 and BPHYT_RS00945 are separated by 197 nucleotides
BPHYT_RS00935: BPHYT_RS00935 - integrase, at 213,540 to 215,057
_RS00935
BPHYT_RS00940: BPHYT_RS00940 - transposase, at 215,263 to 215,730
_RS00940
BPHYT_RS00945: BPHYT_RS00945 - hypothetical protein, at 215,928 to 216,449
_RS00945
Position (kb) 215
216 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 215.263 kb on + strand at 215.550 kb on - strand, within BPHYT_RS00940 at 215.550 kb on - strand, within BPHYT_RS00940 at 215.595 kb on + strand, within BPHYT_RS00940 at 215.621 kb on + strand, within BPHYT_RS00940 at 215.711 kb on + strand at 215.721 kb on - strand at 215.754 kb on + strand at 215.766 kb on + strand at 215.767 kb on - strand at 215.798 kb on + strand at 215.825 kb on - strand at 215.836 kb on - strand at 215.941 kb on - strand at 216.160 kb on - strand, within BPHYT_RS00945 at 216.160 kb on - strand, within BPHYT_RS00945 at 216.163 kb on - strand, within BPHYT_RS00945 at 216.163 kb on - strand, within BPHYT_RS00945 at 216.193 kb on - strand, within BPHYT_RS00945 at 216.193 kb on - strand, within BPHYT_RS00945 at 216.428 kb on + strand at 216.429 kb on - strand at 216.465 kb on + strand at 216.466 kb on - strand at 216.492 kb on + strand at 216.492 kb on + strand at 216.620 kb on + strand at 216.620 kb on + strand at 216.620 kb on + strand at 216.620 kb on + strand at 216.621 kb on - strand at 216.695 kb on + strand at 216.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO4 MOI 1 remove 215,263 + -0.3 215,550 - BPHYT_RS00940 0.61 -1.8 215,550 - BPHYT_RS00940 0.61 -1.9 215,595 + BPHYT_RS00940 0.71 -3.1 215,621 + BPHYT_RS00940 0.76 -1.4 215,711 + -1.4 215,721 - +0.6 215,754 + +0.5 215,766 + +1.3 215,767 - -1.1 215,798 + -0.7 215,825 - +0.2 215,836 - -0.3 215,941 - +0.3 216,160 - BPHYT_RS00945 0.44 +0.2 216,160 - BPHYT_RS00945 0.44 -0.6 216,163 - BPHYT_RS00945 0.45 -0.6 216,163 - BPHYT_RS00945 0.45 -0.6 216,193 - BPHYT_RS00945 0.51 +0.8 216,193 - BPHYT_RS00945 0.51 -0.1 216,428 + +1.2 216,429 - -1.2 216,465 + -0.5 216,466 - -0.3 216,492 + +0.0 216,492 + +0.4 216,620 + -2.5 216,620 + +1.5 216,620 + +0.3 216,620 + +1.5 216,621 - -1.5 216,695 + +0.5 216,711 - -0.2
Or see this region's nucleotide sequence