Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30030 and BPHYT_RS30035 are separated by 116 nucleotides BPHYT_RS30035 and BPHYT_RS30040 are separated by 27 nucleotides BPHYT_RS30040 and BPHYT_RS30045 are separated by 382 nucleotides
BPHYT_RS30030: BPHYT_RS30030 - metallophosphoesterase, at 2,293,160 to 2,294,260
_RS30030
BPHYT_RS30035: BPHYT_RS30035 - hypothetical protein, at 2,294,377 to 2,294,943
_RS30035
BPHYT_RS30040: BPHYT_RS30040 - hypothetical protein, at 2,294,971 to 2,295,534
_RS30040
BPHYT_RS30045: BPHYT_RS30045 - ATPase AAA, at 2,295,917 to 2,297,293
_RS30045
Position (kb)
2294
2295
2296 Strain fitness (log2 ratio)
-2
-1
0
1 at 2294.120 kb on - strand, within BPHYT_RS30030 at 2294.264 kb on + strand at 2294.265 kb on - strand at 2294.378 kb on + strand at 2294.958 kb on - strand at 2294.977 kb on - strand at 2295.418 kb on - strand, within BPHYT_RS30040 at 2295.618 kb on + strand at 2295.918 kb on + strand at 2295.918 kb on + strand at 2295.919 kb on - strand at 2296.222 kb on - strand, within BPHYT_RS30045 at 2296.479 kb on - strand, within BPHYT_RS30045
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,294,120 - BPHYT_RS30030 0.87 -1.9 2,294,264 + +0.2 2,294,265 - +0.7 2,294,378 + -1.2 2,294,958 - -1.9 2,294,977 - +0.4 2,295,418 - BPHYT_RS30040 0.79 -1.4 2,295,618 + +0.7 2,295,918 + -0.0 2,295,918 + -0.4 2,295,919 - +0.3 2,296,222 - BPHYT_RS30045 0.22 -2.3 2,296,479 - BPHYT_RS30045 0.41 +0.5
Or see this region's nucleotide sequence