Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26815 and BPHYT_RS26820 overlap by 4 nucleotides BPHYT_RS26820 and BPHYT_RS26825 are separated by 131 nucleotides BPHYT_RS26825 and BPHYT_RS26830 are separated by 77 nucleotides
BPHYT_RS26815: BPHYT_RS26815 - 1-aminocyclopropane-1-carboxylate deaminase, at 1,588,287 to 1,589,303
_RS26815
BPHYT_RS26820: BPHYT_RS26820 - LysR family transcriptional regulator, at 1,589,300 to 1,590,187
_RS26820
BPHYT_RS26825: BPHYT_RS26825 - quercetin 2,3-dioxygenase, at 1,590,319 to 1,591,044
_RS26825
BPHYT_RS26830: BPHYT_RS26830 - membrane protein, at 1,591,122 to 1,591,535
_RS26830
Position (kb) 1589
1590
1591 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1588.455 kb on + strand, within BPHYT_RS26815 at 1588.753 kb on - strand, within BPHYT_RS26815 at 1588.860 kb on + strand, within BPHYT_RS26815 at 1589.241 kb on + strand at 1589.242 kb on - strand at 1589.277 kb on + strand at 1589.383 kb on - strand at 1589.616 kb on + strand, within BPHYT_RS26820 at 1589.679 kb on + strand, within BPHYT_RS26820 at 1590.139 kb on + strand at 1590.254 kb on + strand at 1590.255 kb on - strand at 1590.628 kb on + strand, within BPHYT_RS26825 at 1590.629 kb on - strand, within BPHYT_RS26825 at 1590.815 kb on - strand, within BPHYT_RS26825 at 1591.063 kb on + strand at 1591.063 kb on + strand at 1591.064 kb on - strand at 1591.130 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,588,455 + BPHYT_RS26815 0.17 -1.3 1,588,753 - BPHYT_RS26815 0.46 +0.7 1,588,860 + BPHYT_RS26815 0.56 -0.4 1,589,241 + -0.8 1,589,242 - -0.3 1,589,277 + +0.6 1,589,383 - +0.1 1,589,616 + BPHYT_RS26820 0.36 -2.9 1,589,679 + BPHYT_RS26820 0.43 -0.7 1,590,139 + -1.0 1,590,254 + -2.4 1,590,255 - -0.7 1,590,628 + BPHYT_RS26825 0.43 -0.7 1,590,629 - BPHYT_RS26825 0.43 +0.8 1,590,815 - BPHYT_RS26825 0.68 -0.3 1,591,063 + +1.0 1,591,063 + -1.3 1,591,064 - -0.0 1,591,130 + +2.2
Or see this region's nucleotide sequence