Experiment: 24hr PHYTO4 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27635 and BPHYT_RS27640 are separated by 431 nucleotides BPHYT_RS27640 and BPHYT_RS27645 are separated by 467 nucleotides BPHYT_RS27645 and BPHYT_RS27650 are separated by 58 nucleotides
BPHYT_RS27635: BPHYT_RS27635 - alcohol dehydrogenase, at 1,767,832 to 1,768,872
_RS27635
BPHYT_RS27640: BPHYT_RS27640 - acetoacetyl-CoA reductase, at 1,769,304 to 1,770,050
_RS27640
BPHYT_RS27645: BPHYT_RS27645 - universal stress protein UspA, at 1,770,518 to 1,770,976
_RS27645
BPHYT_RS27650: BPHYT_RS27650 - hypothetical protein, at 1,771,035 to 1,771,382
_RS27650
Position (kb)
1769
1770
1771 Strain fitness (log2 ratio)
-1
0
1
2 at 1768.509 kb on - strand, within BPHYT_RS27635 at 1768.509 kb on - strand, within BPHYT_RS27635 at 1768.705 kb on - strand, within BPHYT_RS27635 at 1768.856 kb on + strand at 1769.230 kb on + strand at 1769.422 kb on - strand, within BPHYT_RS27640 at 1769.591 kb on - strand, within BPHYT_RS27640 at 1769.961 kb on + strand, within BPHYT_RS27640 at 1770.050 kb on + strand at 1770.051 kb on - strand at 1770.451 kb on - strand at 1770.505 kb on - strand at 1770.981 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO4 MOI 10 remove 1,768,509 - BPHYT_RS27635 0.65 +0.6 1,768,509 - BPHYT_RS27635 0.65 +1.9 1,768,705 - BPHYT_RS27635 0.84 +2.1 1,768,856 + -0.1 1,769,230 + +0.8 1,769,422 - BPHYT_RS27640 0.16 +0.2 1,769,591 - BPHYT_RS27640 0.38 +0.1 1,769,961 + BPHYT_RS27640 0.88 -0.4 1,770,050 + +0.5 1,770,051 - -0.0 1,770,451 - -0.8 1,770,505 - +0.7 1,770,981 - +0.7
Or see this region's nucleotide sequence