Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07085 and BPHYT_RS07090 are separated by 127 nucleotides BPHYT_RS07090 and BPHYT_RS07095 overlap by 31 nucleotides BPHYT_RS07095 and BPHYT_RS07100 are separated by 132 nucleotides
BPHYT_RS07085: BPHYT_RS07085 - LysR family transcriptional regulator, at 1,601,573 to 1,602,496
_RS07085
BPHYT_RS07090: BPHYT_RS07090 - hypothetical protein, at 1,602,624 to 1,602,941
_RS07090
BPHYT_RS07095: BPHYT_RS07095 - LysR family transcriptional regulator, at 1,602,911 to 1,603,837
_RS07095
BPHYT_RS07100: BPHYT_RS07100 - isovaleryl-CoA dehydrogenase, at 1,603,970 to 1,605,736
_RS07100
Position (kb)
1602
1603
1604 Strain fitness (log2 ratio)
-1
0
1
2 at 1602.200 kb on + strand, within BPHYT_RS07085 at 1602.377 kb on + strand at 1602.565 kb on + strand at 1602.565 kb on + strand at 1602.901 kb on + strand, within BPHYT_RS07090 at 1602.913 kb on - strand at 1602.978 kb on + strand at 1603.530 kb on + strand, within BPHYT_RS07095 at 1603.887 kb on + strand at 1603.888 kb on - strand at 1603.920 kb on + strand at 1603.997 kb on + strand at 1604.193 kb on + strand, within BPHYT_RS07100 at 1604.194 kb on - strand, within BPHYT_RS07100
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 1,602,200 + BPHYT_RS07085 0.68 +0.4 1,602,377 + -0.0 1,602,565 + +0.5 1,602,565 + -0.0 1,602,901 + BPHYT_RS07090 0.87 +0.1 1,602,913 - +0.7 1,602,978 + +0.9 1,603,530 + BPHYT_RS07095 0.67 -1.1 1,603,887 + +0.2 1,603,888 - +1.7 1,603,920 + +1.1 1,603,997 + -0.6 1,604,193 + BPHYT_RS07100 0.13 -0.9 1,604,194 - BPHYT_RS07100 0.13 +2.3
Or see this region's nucleotide sequence