Experiment: K. M5a1 KLEB10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotides BWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotides BWI76_RS12835 and BWI76_RS12840 are separated by 27 nucleotides
BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619
_RS12825
BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454
_RS12830
BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350
_RS12835
BWI76_RS12840: BWI76_RS12840 - amino acid ABC transporter permease/ATP-binding protein, at 2,675,378 to 2,676,898
_RS12840
Position (kb)
2674
2675
2676 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2674.325 kb on + strand, within BWI76_RS12830 at 2674.345 kb on - strand, within BWI76_RS12830 at 2674.382 kb on - strand at 2674.421 kb on + strand at 2674.667 kb on - strand, within BWI76_RS12835 at 2674.678 kb on - strand, within BWI76_RS12835 at 2674.855 kb on - strand, within BWI76_RS12835 at 2674.992 kb on - strand, within BWI76_RS12835 at 2675.088 kb on - strand, within BWI76_RS12835 at 2675.189 kb on + strand, within BWI76_RS12835 at 2675.572 kb on - strand, within BWI76_RS12840 at 2675.688 kb on + strand, within BWI76_RS12840 at 2675.688 kb on + strand, within BWI76_RS12840 at 2675.816 kb on + strand, within BWI76_RS12840 at 2675.977 kb on + strand, within BWI76_RS12840 at 2675.977 kb on + strand, within BWI76_RS12840 at 2676.009 kb on + strand, within BWI76_RS12840 at 2676.312 kb on + strand, within BWI76_RS12840 at 2676.320 kb on - strand, within BWI76_RS12840
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB10 MOI 1 remove 2,674,325 + BWI76_RS12830 0.84 +1.2 2,674,345 - BWI76_RS12830 0.87 +1.4 2,674,382 - -1.0 2,674,421 + +1.7 2,674,667 - BWI76_RS12835 0.22 +0.2 2,674,678 - BWI76_RS12835 0.23 +0.0 2,674,855 - BWI76_RS12835 0.43 +2.3 2,674,992 - BWI76_RS12835 0.59 +1.4 2,675,088 - BWI76_RS12835 0.70 -0.4 2,675,189 + BWI76_RS12835 0.81 +1.0 2,675,572 - BWI76_RS12840 0.13 +0.6 2,675,688 + BWI76_RS12840 0.20 -2.0 2,675,688 + BWI76_RS12840 0.20 -2.3 2,675,816 + BWI76_RS12840 0.29 -1.0 2,675,977 + BWI76_RS12840 0.39 -0.4 2,675,977 + BWI76_RS12840 0.39 +0.4 2,676,009 + BWI76_RS12840 0.41 -0.5 2,676,312 + BWI76_RS12840 0.61 -1.1 2,676,320 - BWI76_RS12840 0.62 -0.7
Or see this region's nucleotide sequence