Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18180

Experiment: B3 MOi 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18170 and BWI76_RS18175 are separated by 268 nucleotidesBWI76_RS18175 and BWI76_RS18180 overlap by 4 nucleotidesBWI76_RS18180 and BWI76_RS18185 are separated by 53 nucleotidesBWI76_RS18185 and BWI76_RS18190 are separated by 39 nucleotides BWI76_RS18170: BWI76_RS18170 - aspartate--tRNA ligase, at 3,774,895 to 3,776,682 _RS18170 BWI76_RS18175: BWI76_RS18175 - hydrolase, at 3,776,951 to 3,777,517 _RS18175 BWI76_RS18180: BWI76_RS18180 - hypothetical protein, at 3,777,514 to 3,778,332 _RS18180 BWI76_RS18185: BWI76_RS18185 - membrane protein, at 3,778,386 to 3,778,781 _RS18185 BWI76_RS18190: BWI76_RS18190 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 3,778,821 to 3,779,564 _RS18190 Position (kb) 3777 3778 3779Strain fitness (log2 ratio) -2 -1 0 1at 3776.813 kb on + strandat 3776.909 kb on + strandat 3776.914 kb on + strandat 3776.943 kb on + strandat 3777.093 kb on + strand, within BWI76_RS18175at 3777.177 kb on + strand, within BWI76_RS18175at 3777.284 kb on - strand, within BWI76_RS18175at 3777.367 kb on + strand, within BWI76_RS18175at 3777.471 kb on + strandat 3777.489 kb on + strandat 3777.489 kb on + strandat 3777.583 kb on - strandat 3777.748 kb on + strand, within BWI76_RS18180at 3777.799 kb on + strand, within BWI76_RS18180at 3777.930 kb on + strand, within BWI76_RS18180at 3777.977 kb on - strand, within BWI76_RS18180at 3778.454 kb on + strand, within BWI76_RS18185at 3778.478 kb on - strand, within BWI76_RS18185at 3778.484 kb on - strand, within BWI76_RS18185at 3778.603 kb on - strand, within BWI76_RS18185at 3778.605 kb on + strand, within BWI76_RS18185at 3778.649 kb on + strand, within BWI76_RS18185at 3778.744 kb on + strandat 3778.752 kb on - strandat 3779.110 kb on - strand, within BWI76_RS18190at 3779.150 kb on + strand, within BWI76_RS18190

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Per-strain Table

Position Strand Gene LocusTag Fraction B3 MOi 10
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3,776,813 + +0.2
3,776,909 + +0.4
3,776,914 + -0.8
3,776,943 + -1.0
3,777,093 + BWI76_RS18175 0.25 +0.3
3,777,177 + BWI76_RS18175 0.40 -1.5
3,777,284 - BWI76_RS18175 0.59 -0.5
3,777,367 + BWI76_RS18175 0.73 -0.3
3,777,471 + -0.0
3,777,489 + +0.6
3,777,489 + +1.0
3,777,583 - -1.9
3,777,748 + BWI76_RS18180 0.29 -0.3
3,777,799 + BWI76_RS18180 0.35 -0.4
3,777,930 + BWI76_RS18180 0.51 -1.2
3,777,977 - BWI76_RS18180 0.57 -0.6
3,778,454 + BWI76_RS18185 0.17 -0.0
3,778,478 - BWI76_RS18185 0.23 -0.4
3,778,484 - BWI76_RS18185 0.25 -0.8
3,778,603 - BWI76_RS18185 0.55 -0.3
3,778,605 + BWI76_RS18185 0.55 -0.3
3,778,649 + BWI76_RS18185 0.66 +0.5
3,778,744 + +1.3
3,778,752 - +0.6
3,779,110 - BWI76_RS18190 0.39 +0.6
3,779,150 + BWI76_RS18190 0.44 -0.8

Or see this region's nucleotide sequence