Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14250 and BWI76_RS14255 overlap by 8 nucleotides BWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotides BWI76_RS14260 and BWI76_RS14265 are separated by 158 nucleotides
BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781
_RS14250
BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778
_RS14255
BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930
_RS14260
BWI76_RS14265: BWI76_RS14265 - LysR family transcriptional regulator, at 2,983,089 to 2,984,027
_RS14265
Position (kb)
2981
2982
2983 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2980.778 kb on + strand, within BWI76_RS14250 at 2980.851 kb on - strand, within BWI76_RS14250 at 2980.870 kb on - strand, within BWI76_RS14250 at 2980.872 kb on - strand, within BWI76_RS14250 at 2980.875 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.926 kb on - strand, within BWI76_RS14250 at 2980.935 kb on - strand, within BWI76_RS14250 at 2981.037 kb on - strand, within BWI76_RS14250 at 2981.158 kb on - strand, within BWI76_RS14250 at 2981.255 kb on + strand, within BWI76_RS14250 at 2981.255 kb on + strand, within BWI76_RS14250 at 2981.263 kb on - strand at 2981.331 kb on - strand, within BWI76_RS14250 at 2981.362 kb on - strand, within BWI76_RS14250 at 2981.362 kb on - strand, within BWI76_RS14250 at 2981.383 kb on + strand, within BWI76_RS14250 at 2981.565 kb on - strand, within BWI76_RS14250 at 2981.581 kb on - strand, within BWI76_RS14250 at 2981.625 kb on + strand, within BWI76_RS14250 at 2981.635 kb on + strand, within BWI76_RS14250 at 2981.643 kb on - strand at 2981.697 kb on + strand at 2981.726 kb on + strand at 2981.736 kb on + strand at 2981.744 kb on - strand at 2981.824 kb on + strand at 2982.006 kb on + strand, within BWI76_RS14255 at 2982.162 kb on + strand, within BWI76_RS14255 at 2982.170 kb on - strand, within BWI76_RS14255 at 2982.227 kb on - strand, within BWI76_RS14255 at 2982.232 kb on - strand, within BWI76_RS14255 at 2982.253 kb on + strand, within BWI76_RS14255 at 2982.261 kb on - strand, within BWI76_RS14255 at 2982.271 kb on + strand, within BWI76_RS14255 at 2982.297 kb on + strand, within BWI76_RS14255 at 2982.366 kb on - strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.854 kb on - strand, within BWI76_RS14260 at 2982.957 kb on + strand at 2982.974 kb on - strand at 2983.006 kb on - strand at 2983.024 kb on + strand at 2983.032 kb on + strand at 2983.039 kb on - strand at 2983.039 kb on - strand at 2983.055 kb on - strand at 2983.171 kb on + strand at 2983.225 kb on + strand, within BWI76_RS14265 at 2983.372 kb on - strand, within BWI76_RS14265 at 2983.406 kb on - strand, within BWI76_RS14265 at 2983.534 kb on + strand, within BWI76_RS14265 at 2983.636 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.765 kb on - strand, within BWI76_RS14265
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 2,980,778 + BWI76_RS14250 0.29 -0.1 2,980,851 - BWI76_RS14250 0.34 +0.4 2,980,870 - BWI76_RS14250 0.36 +2.7 2,980,872 - BWI76_RS14250 0.36 -0.1 2,980,875 + BWI76_RS14250 0.36 +0.1 2,980,918 + BWI76_RS14250 0.39 -0.0 2,980,918 + BWI76_RS14250 0.39 -1.7 2,980,926 - BWI76_RS14250 0.40 -0.8 2,980,935 - BWI76_RS14250 0.40 +0.3 2,981,037 - BWI76_RS14250 0.47 +2.1 2,981,158 - BWI76_RS14250 0.56 -0.5 2,981,255 + BWI76_RS14250 0.63 -0.3 2,981,255 + BWI76_RS14250 0.63 +0.2 2,981,263 - -0.1 2,981,331 - BWI76_RS14250 0.68 -0.3 2,981,362 - BWI76_RS14250 0.70 +0.0 2,981,362 - BWI76_RS14250 0.70 -0.8 2,981,383 + BWI76_RS14250 0.72 +1.0 2,981,565 - BWI76_RS14250 0.85 +0.9 2,981,581 - BWI76_RS14250 0.86 -0.4 2,981,625 + BWI76_RS14250 0.89 -0.6 2,981,635 + BWI76_RS14250 0.90 -0.3 2,981,643 - -0.1 2,981,697 + -0.0 2,981,726 + -0.7 2,981,736 + +2.5 2,981,744 - +2.2 2,981,824 + +2.2 2,982,006 + BWI76_RS14255 0.23 -0.6 2,982,162 + BWI76_RS14255 0.39 +0.1 2,982,170 - BWI76_RS14255 0.39 +0.5 2,982,227 - BWI76_RS14255 0.45 -0.0 2,982,232 - BWI76_RS14255 0.46 +0.2 2,982,253 + BWI76_RS14255 0.48 +0.6 2,982,261 - BWI76_RS14255 0.48 -0.0 2,982,271 + BWI76_RS14255 0.49 -0.0 2,982,297 + BWI76_RS14255 0.52 -0.2 2,982,366 - BWI76_RS14255 0.59 -1.7 2,982,488 + BWI76_RS14255 0.71 -0.4 2,982,488 + BWI76_RS14255 0.71 +2.1 2,982,854 - BWI76_RS14260 0.51 +0.1 2,982,957 + -0.2 2,982,974 - +0.4 2,983,006 - +0.6 2,983,024 + -2.3 2,983,032 + -0.4 2,983,039 - -0.4 2,983,039 - -0.6 2,983,055 - +0.3 2,983,171 + +3.5 2,983,225 + BWI76_RS14265 0.14 +0.6 2,983,372 - BWI76_RS14265 0.30 +0.9 2,983,406 - BWI76_RS14265 0.34 -1.7 2,983,534 + BWI76_RS14265 0.47 -0.7 2,983,636 + BWI76_RS14265 0.58 -0.0 2,983,757 + BWI76_RS14265 0.71 +0.9 2,983,757 + BWI76_RS14265 0.71 -0.7 2,983,757 + BWI76_RS14265 0.71 +0.9 2,983,765 - BWI76_RS14265 0.72 -0.1
Or see this region's nucleotide sequence