Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04555 and BWI76_RS04560 are separated by 168 nucleotides BWI76_RS04560 and BWI76_RS04565 are separated by 81 nucleotides BWI76_RS04565 and BWI76_RS04570 are separated by 12 nucleotides
BWI76_RS04555: BWI76_RS04555 - citrate/acetate antiporter, at 943,966 to 945,321
_RS04555
BWI76_RS04560: BWI76_RS04560 - holo-ACP synthase CitX, at 945,490 to 946,029
_RS04560
BWI76_RS04565: BWI76_RS04565 - bifunctional tRNA pseudouridine(32) synthase/ribosomal large subunit pseudouridine synthase RluA, at 946,111 to 946,770
_RS04565
BWI76_RS04570: BWI76_RS04570 - RNA polymerase-associated protein RapA, at 946,783 to 949,689
_RS04570
Position (kb) 946
947 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 945.187 kb on - strand at 945.525 kb on - strand at 945.626 kb on - strand, within BWI76_RS04560 at 945.644 kb on + strand, within BWI76_RS04560 at 945.773 kb on - strand, within BWI76_RS04560 at 945.809 kb on - strand, within BWI76_RS04560 at 946.053 kb on - strand at 946.098 kb on - strand at 946.108 kb on - strand at 946.342 kb on + strand, within BWI76_RS04565 at 946.398 kb on + strand, within BWI76_RS04565 at 946.407 kb on + strand, within BWI76_RS04565 at 946.474 kb on - strand, within BWI76_RS04565 at 946.543 kb on + strand, within BWI76_RS04565 at 946.554 kb on + strand, within BWI76_RS04565 at 947.054 kb on + strand at 947.054 kb on + strand at 947.129 kb on - strand, within BWI76_RS04570 at 947.349 kb on + strand, within BWI76_RS04570 at 947.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 945,187 - +1.0 945,525 - +0.9 945,626 - BWI76_RS04560 0.25 -0.6 945,644 + BWI76_RS04560 0.29 +0.6 945,773 - BWI76_RS04560 0.52 +0.5 945,809 - BWI76_RS04560 0.59 +0.1 946,053 - +0.7 946,098 - -1.1 946,108 - -2.0 946,342 + BWI76_RS04565 0.35 -0.8 946,398 + BWI76_RS04565 0.43 +0.5 946,407 + BWI76_RS04565 0.45 -0.6 946,474 - BWI76_RS04565 0.55 +1.0 946,543 + BWI76_RS04565 0.65 -1.4 946,554 + BWI76_RS04565 0.67 +0.0 947,054 + +2.9 947,054 + +1.0 947,129 - BWI76_RS04570 0.12 +0.3 947,349 + BWI76_RS04570 0.19 -1.8 947,595 - +1.0
Or see this region's nucleotide sequence