Experiment: KLEB31 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00920 and BWI76_RS00925 are separated by 54 nucleotides BWI76_RS00925 and BWI76_RS00930 are separated by 55 nucleotides BWI76_RS00930 and BWI76_RS00935 are separated by 54 nucleotides BWI76_RS00935 and BWI76_RS00940 are separated by 82 nucleotides BWI76_RS00940 and BWI76_RS00945 are separated by 118 nucleotides BWI76_RS00945 and BWI76_RS00950 are separated by 23 nucleotides
BWI76_RS00920: BWI76_RS00920 - 23S ribosomal RNA, at 188,536 to 191,462
_RS00920
BWI76_RS00925: BWI76_RS00925 - 5S ribosomal RNA, at 191,517 to 191,632
_RS00925
BWI76_RS00930: BWI76_RS00930 - tRNA-Asp, at 191,688 to 191,764
_RS00930
BWI76_RS00935: BWI76_RS00935 - tRNA-Trp, at 191,819 to 191,894
_RS00935
BWI76_RS00940: BWI76_RS00940 - transcriptional regulator HdfR, at 191,977 to 192,798
_RS00940
BWI76_RS00945: BWI76_RS00945 - hypothetical protein, at 192,917 to 193,255
_RS00945
BWI76_RS00950: BWI76_RS00950 - ATP-dependent protease, at 193,279 to 194,799
_RS00950
Position (kb)
191
192
193 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 192.066 kb on + strand, within BWI76_RS00940 at 192.123 kb on - strand, within BWI76_RS00940 at 192.126 kb on + strand, within BWI76_RS00940 at 192.126 kb on + strand, within BWI76_RS00940 at 192.131 kb on + strand, within BWI76_RS00940 at 192.136 kb on + strand, within BWI76_RS00940 at 192.138 kb on + strand, within BWI76_RS00940 at 192.263 kb on - strand, within BWI76_RS00940 at 192.413 kb on + strand, within BWI76_RS00940 at 192.461 kb on + strand, within BWI76_RS00940 at 192.461 kb on + strand, within BWI76_RS00940 at 192.620 kb on + strand, within BWI76_RS00940 at 192.773 kb on - strand at 192.853 kb on + strand at 192.863 kb on - strand at 192.866 kb on + strand at 192.928 kb on - strand at 193.018 kb on + strand, within BWI76_RS00945 at 193.602 kb on - strand, within BWI76_RS00950 at 193.745 kb on - strand, within BWI76_RS00950
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB31 MOI 1 remove 192,066 + BWI76_RS00940 0.11 +0.4 192,123 - BWI76_RS00940 0.18 +1.2 192,126 + BWI76_RS00940 0.18 +3.0 192,126 + BWI76_RS00940 0.18 +1.4 192,131 + BWI76_RS00940 0.19 +0.5 192,136 + BWI76_RS00940 0.19 +1.1 192,138 + BWI76_RS00940 0.20 -0.7 192,263 - BWI76_RS00940 0.35 +2.2 192,413 + BWI76_RS00940 0.53 -0.9 192,461 + BWI76_RS00940 0.59 +1.8 192,461 + BWI76_RS00940 0.59 +1.1 192,620 + BWI76_RS00940 0.78 +0.6 192,773 - +0.9 192,853 + +0.9 192,863 - +1.8 192,866 + -2.3 192,928 - +4.4 193,018 + BWI76_RS00945 0.30 +0.6 193,602 - BWI76_RS00950 0.21 +0.7 193,745 - BWI76_RS00950 0.31 +0.1
Or see this region's nucleotide sequence